Anti-Cdk8抗体(ab115155)
Key features and details
- Rabbit polyclonal to Cdk8
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Cdk8抗体
参阅全部 Cdk8 一抗 -
描述
兔多克隆抗体to Cdk8 -
宿主
Rabbit -
经测试应用
适用于: IPmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rabbit, Horse, Chicken, Cow, Dog, Turkey, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Tilapia, Orangutan, Xenopus tropicalis, Medaka fish -
免疫原
Synthetic peptide, corresponding to a region within amino acids 370-420 of Human Cdk8 (P49336).
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阳性对照
- HeLa whole cell lysate (ab150035).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Affinity purified using an epitope specific to CDK8 immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab115155于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IP |
Use at 2-5 µg/mg of lysate.
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说明 |
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IP
Use at 2-5 µg/mg of lysate. |
靶标
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功能
Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Phosphorylates CCNH leading to down-regulation of the TFIIH complex and transcriptional repression. Recruited through interaction with MAML1 to hyperphosphorylate the intracellular domain of NOTCH, leading to its degradation. -
序列相似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 1024 Human
- Entrez Gene: 264064 Mouse
- Entrez Gene: 447409 Xenopus laevis
- Entrez Gene: 394856 Xenopus tropicalis
- Omim: 603184 Human
- SwissProt: P49336 Human
- SwissProt: Q8R3L8 Mouse
- SwissProt: Q66KH9 Xenopus laevis
see all -
别名
- cdk8 antibody
- CDK8 protein kinase antibody
- CDK8_HUMAN antibody
see all
图片
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Immunoprecipitation of Cdk8 (3 µg of antibody per mg of HeLa cells lysate; 20% of the IP loaded). Lane 1- Immunoprecipitation with anti-Cdk8 antibody (ab115155); Lane 2-Immunoprecipitation with an anti-Cdk8 antibody that recognizes an upstream epitope; Lane 3- Immunoprecipitation with IgG control. An anti-Cdk8 antibody used in lane 2 was used at 0.4 µg/ml for western blot. Detection: Chemiluminescence with exposure time of 30 sec.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab115155 被引用在 2 文献中.
- Zhang Q & Chen Z lncRNA UASR1 sponges miR-107 in colorectal cancer to upregulate oncogenic CDK8 and promote cell proliferation. Oncol Lett 20:305 (2020). PubMed: 33093914
- Mediouni S et al. Didehydro-Cortistatin A Inhibits HIV-1 by Specifically Binding to the Unstructured Basic Region of Tat. MBio 10:N/A (2019). PubMed: 30723126