Key features and details
- Mouse monoclonal [IMD-26] to Cdk7
- Suitable for: WB, IHC-P, ICC/IF, IHC-Fr
- Reacts with: Human
- Isotype: IgG2b
存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
存储溶液Preservative: 0.001% Sodium azide
Constituents: 98% PBS, 0.2% BSA, 1.2% Sodium acetate
Concentration information loading...
纯度Protein G purified
Our Abpromise guarantee covers the use of ab130001 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.25 µg/ml. Predicted molecular weight: 39 kDa.|
|IHC-P||Use a concentration of 0.5 µg/ml.|
|ICC/IF||Use a concentration of 1 µg/ml.|
|IHC-Fr||Use a concentration of 0.5 µg/ml.|
功能Cyclin-dependent kinases (CDKs) are activated by the binding to a cyclin and mediate the progression through the cell cycle. Each different complex controls a specific transition between two subsequent phases in the cell cycle. CDK7 is the catalytic subunit of the CDK-activating kinase (CAK) complex, a serine-threonine kinase. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminus domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle.
序列相似性Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain.
翻译后修饰Phosphorylation of Ser-164 during mitosis inactivates the enzyme.
Phosphorylation of Thr-170 is required for activity.
- Information by UniProt
- 39 kDa protein kinase antibody
- CAK antibody
- CAK1 antibody