Anti-CDK5RAP3抗体(ab70776)
Key features and details
- Rabbit polyclonal to CDK5RAP3
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-CDK5RAP3抗体
参阅全部 CDK5RAP3 一抗 -
描述
兔多克隆抗体to CDK5RAP3 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Chimpanzee, Rhesus monkey, Chinese hamster, Orangutan, Platypus -
免疫原
Synthetic peptide corresponding to a region between residue 450 and the C-terminus (residue 506) of human CDK5RAP3 (NP_788276.1)
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阳性对照
- HeLa and 293T whole cell lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab70776 was affinity purified using an epitope specific to CDK5RAP3 immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab70776于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/2000 - 1/10000. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
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IP |
Use at 2-5 µg/mg of lysate.
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说明 |
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WB
1/2000 - 1/10000. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
靶标
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功能
Potential regulator of CDK5 activity. May be involved in cell proliferation. Regulates CDK5 activity via its interaction with CDK5R1. -
组织特异性
Ubiquitous. Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform 3 is expressed in kidney, liver, skeletal muscle and placenta. -
序列相似性
Belongs to the CDK5RAP3 family. -
翻译后修饰
Phosphorylated in vitro by CDK5. - Information by UniProt
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数据库链接
- Entrez Gene: 80279 Human
- Entrez Gene: 80280 Mouse
- Entrez Gene: 695189 Rhesus monkey
- Omim: 608202 Human
- SwissProt: Q96JB5 Human
- SwissProt: Q99LM2 Mouse
- Unigene: 20157 Human
- Unigene: 28297 Mouse
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别名
- C53 antibody
- CDK5 activator binding protein C53 antibody
- CDK5 activator-binding protein C53 antibody
see all
图片
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All lanes : Anti-CDK5RAP3 antibody (ab70776) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 57 kDa
Observed band size: 57 kDa
Additional bands at: 220 kDa, 28 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes -
Detection of CDK5RAP3 by Western Blot of Immunprecipitate. ab70776 at 1µg/ml staining CDK5RAP3 in HeLa whole cell lysate immunoprecipitated using ab70776 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Chemiluminescence with exposure time of 30 seconds.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab70776 被引用在 2 文献中.
- Egusquiaguirre SP et al. CDK5RAP3 is a co-factor for the oncogenic transcription factor STAT3. Neoplasia 22:47-59 (2020). PubMed: 31765941
- Horejší B et al. Nuclear ?-tubulin associates with nucleoli and interacts with tumor suppressor protein C53. J Cell Physiol 227:367-82 (2012). PubMed: 21465471