重组Anti-CDK2 (phospho T14)抗体[EP2234Y] (ab68265)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2234Y] to CDK2 (phospho T14)
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human, Chinese hamster
Related conjugates and formulations
概述
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产品名称
Anti-CDK2 (phospho T14)抗体[EP2234Y]
参阅全部 CDK2 一抗 -
描述
兔单克隆抗体[EP2234Y] to CDK2 (phospho T14) -
宿主
Rabbit -
特异性
This antibody detects Cdk2 phosphorylated on Threonine 14.
The Chinese hamster recommendation is based on the WB result. We do not guarantee IHC-P for Chinese hamster.
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经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt -
种属反应性
与反应: Mouse, Rat, Human, Chinese hamster -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HeLa,C2C12 (Mouse myoblasts myoblast) treated with 1mM hydroxyurea for 16 hours, C6 (Rat glial tumor glial cell) treated with 1mM hydroxyurea for 16 hours, CHO-S (Chinese hamster ovary epithelial cell) whole cell lysates. IHC: Human ovarian carcinoma, mouse and rat stomach tissues.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP2234Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab68265于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
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IHC-P |
1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
The Chinese hamster recommendation is based on the WB result. We do not guarantee IHC-P for Chinese hamster. |
说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa). |
IHC-P
1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. The Chinese hamster recommendation is based on the WB result. We do not guarantee IHC-P for Chinese hamster. |
靶标
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功能
Involved in the control of the cell cycle. Interacts with cyclins A, B1, B3, D, or E. Activity of CDK2 is maximal during S phase and G2. -
序列相似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain. - Information by UniProt
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数据库链接
- Entrez Gene: 100689039 Chinese hamster
- Entrez Gene: 1017 Human
- Entrez Gene: 12566 Mouse
- Entrez Gene: 362817 Rat
- Omim: 116953 Human
- SwissProt: O55076 Chinese hamster
- SwissProt: P24941 Human
- SwissProt: P97377 Mouse
see all -
别名
- Cdc2 related protein kinase antibody
- cdc2-related protein kinase antibody
- CDC28 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat stomach tissue sections labeling CDK2 with Purified 68265 at 1:1000 dilution (0.24 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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All lanes : Anti-CDK2 (phospho T14) antibody [EP2234Y] (ab68265) at 1/1000 dilution (Purified)
Lane 1 : Untreated HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) starved overnight, then treated with 3mM hydroxyurea for 24 hours whole cell lysate
Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) starved overnight, then treated with 3mM hydroxyurea for 24 hours whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 34 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse stomach tissue sections labeling CDK2 with Purified 68265 at 1:1000 dilution (0.24 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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All lanes : Anti-CDK2 (phospho T14) antibody [EP2234Y] (ab68265) at 1/1000 dilution (Purified)
Lane 1 : CHO-S (Chinese hamster ovary epithelial cell) whole cell lysate
Lane 2 : CHO-S (Chinese hamster ovary epithelial cell) whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 34 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue sections labeling CDK2 with Purified 68265 at 1:1000 dilution (0.24 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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All lanes : Anti-CDK2 (phospho T14) antibody [EP2234Y] (ab68265) at 1/1000 dilution (Purified)
Lane 1 : Untreated C6 (Rat glial tumor glial cell) whole cell lysate
Lane 2 : C6 (Rat glial tumor glial cell) treated with 1mM hydroxyurea for 16 hours whole cell lysate
Lane 3 : C6 (Rat glial tumor glial cell) treated with 1mM hydroxyurea for 16 hours whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour
Lane 4 : Untreated C2C12 (Mouse myoblasts myoblast) whole cell lysate
Lane 5 : C2C12 (Mouse myoblasts myoblast) treated with 1mM hydroxyurea for 16 hours whole cell lysate
Lane 6 : C2C12 (Mouse myoblasts myoblast) treated with 1mM hydroxyurea for 16 hours whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 34 kDa
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (11)
ab68265 被引用在 11 文献中.
- Hu Y et al. Phosphoproteomics Reveals Key Regulatory Kinases and Modulated Pathways Associated with Ovarian Cancer Tumors. Onco Targets Ther 13:3595-3605 (2020). PubMed: 32425555
- Patel A et al. Adefovir dipivoxil induces DNA replication stress and augments ATR inhibitor-related cytotoxicity. Int J Cancer 147:1474-1484 (2020). PubMed: 32159854
- Yeh HT et al. Flavopereirine induces cell cycle arrest and apoptosis via the AKT/p38 MAPK/ERK1/2 signaling pathway in human breast cancer cells. Eur J Pharmacol 863:172658 (2019). PubMed: 31518562
- Kohama Y et al. Regulation of the stability and activity of CDC25A and CDC25B by protein phosphatase PP2A and 14-3-3 binding. Cell Signal 54:10-16 (2019). PubMed: 30468767
- Lim EG et al. Ethanol extract from Cnidium monnieri (L.) Cusson induces cell cycle arrest and apoptosis via regulation of the p53-independent pathway in HepG2 and Hep3B hepatocellular carcinoma cells. Mol Med Rep 17:2572-2580 (2018). PubMed: 29207130