Anti-Cdk2抗体(ab235941)
Key features and details
- Rabbit polyclonal to Cdk2
- Suitable for: WB, IP, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Cdk2抗体
参阅全部 CDK2 一抗 -
描述
兔多克隆抗体to Cdk2 -
宿主
Rabbit -
经测试应用
适用于: WB, IP, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow, Chinese hamster, Syrian hamster -
免疫原
Recombinant full length protein corresponding to Human CDK2 aa 1 to the C-terminus.
Database link: P24941 -
阳性对照
- WB: K562, HEK-293, HeLa, Jurkat and HepG2 whole cell lysates. IHC-P: Human tonsil and colon cancer tissues. ICC/IF: HepG2 cells. IP: HeLa whole cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Protein G purified -
纯化说明
Purity >95%. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab235941于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 34 kDa.
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IP |
1/200 - 1/2000.
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IHC-P |
1/500 - 1/1000.
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ICC/IF |
1/50 - 1/500.
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说明 |
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WB
1/1000 - 1/5000. Predicted molecular weight: 34 kDa. |
IP
1/200 - 1/2000. |
IHC-P
1/500 - 1/1000. |
ICC/IF
1/50 - 1/500. |
靶标
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功能
Involved in the control of the cell cycle. Interacts with cyclins A, B1, B3, D, or E. Activity of CDK2 is maximal during S phase and G2. -
序列相似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain. - Information by UniProt
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数据库链接
- Entrez Gene: 100689039 Chinese hamster
- Entrez Gene: 519217 Cow
- Entrez Gene: 1017 Human
- Entrez Gene: 12566 Mouse
- Entrez Gene: 362817 Rat
- Omim: 116953 Human
- SwissProt: O55076 Chinese hamster
- SwissProt: Q5E9Y0 Cow
see all -
别名
- Cdc2 related protein kinase antibody
- cdc2-related protein kinase antibody
- CDC28 antibody
see all
图片
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All lanes : Anti-Cdk2 antibody (ab235941) at 1/1000 dilution
Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 2 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 5 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 34 kDa -
Paraffin-embedded human colon cancer tissue stained for Cdk2 using ab235941 at 1/800 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at room temperature. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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HepG2 (human liver hepatocellular carcinoma cell line) cells labeling Cdk2 using ab235941 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
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Paraffin-embedded human tonsil tissue stained for Cdk2 using ab235941 at 1/800 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at room temperature. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Cdk2 was immunoprecipitated from 0.5 mg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract with ab235941 at 1/200 dilution.
Lane 1: Control rabbit IgG IP (1 μg) in HeLa whole cell lysate.
Lane 2: ab235941 IP in HeLa whole cell extract.
Lane 3: HeLa whole cell lysate 10 μg (Input).
For western blotting, an HRP-conjugated light chain specific antibody was used as the secondary antibody at 1/50000 dilution.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (8)
ab235941 被引用在 8 文献中.
- Pan S et al. SOX4 induces drug resistance of colorectal cancer cells by downregulating CYLD through transcriptional activation of microRNA-17. J Biochem Mol Toxicol 36:e22910 (2022). PubMed: 34927777
- Yu L et al. Blockage of AMPK-ULK1 pathway mediated autophagy promotes cell apoptosis to increase doxorubicin sensitivity in breast cancer (BC) cells: an in vitro study. BMC Cancer 21:195 (2021). PubMed: 33632157
- Xu Q et al. All-trans retinoic acid controls differentiation, proliferation, and lipolysis in isolated subcutaneous adipocytes from peripartal Holstein cows. J Dairy Sci 104:4999-5008 (2021). PubMed: 33551168
- Zhu X et al. DLX6-AS1 accelerates cell proliferation through regulating miR-497-5p/SNCG pathway in prostate cancer. Environ Toxicol 36:308-319 (2021). PubMed: 33035382
- Huang R et al. The activation of GPER inhibits cells proliferation, invasion and EMT of triple-negative breast cancer via CD151/miR-199a-3p bio-axis. Am J Transl Res 12:32-44 (2020). PubMed: 32051735
- Han JB et al. MiR-214 Mediates Cell Proliferation and Apoptosis of Nasopharyngeal Carcinoma Through Targeting Both WWOX and PTEN. Cancer Biother Radiopharm 35:615-625 (2020). PubMed: 32101017
- Wang F et al. Degradation of CCNB1 mediated by APC11 through UBA52 ubiquitination promotes cell cycle progression and proliferation of non-small cell lung cancer cells. Am J Transl Res 11:7166-7185 (2019). PubMed: 31814919
- Cheng Y et al. Knockdown Of lncRNA NCK-AS1 Regulates Cisplatin Resistance Through Modulating miR-137 In Osteosarcoma Cells. Onco Targets Ther 12:11057-11068 (2019). PubMed: 31908475