Anti-Cdc25B (phospho S323)抗体(ab53103)
Key features and details
- Rabbit polyclonal to Cdc25B (phospho S323)
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
概述
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产品名称
Anti-Cdc25B (phospho S323)抗体
参阅全部 Cdc25B 一抗 -
描述
兔多克隆抗体to Cdc25B (phospho S323) -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse -
免疫原
Synthetic peptide corresponding to Human Cdc25B aa 250-350 (phospho S323).
Database link: P30305 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab53103于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/300 - 1/1000. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).
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说明 |
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WB
1/300 - 1/1000. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa). |
靶标
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功能
Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity. -
序列相似性
Belongs to the MPI phosphatase family.
Contains 1 rhodanese domain. -
翻译后修饰
Phosphorylated by BRSK1 in vitro. Phosphorylated by CHEK1, which inhibits the activity of this protein. Phosphorylation at Ser-353 by AURKA might locally participate in the control of the onset of mitosis. Phosphorylation by MELK at Ser-169 promotes localization to the centrosome and the spindle poles during mitosis. Phosphorylation at Ser-323 and Ser-375 by MAPK14 is required for binding to 14-3-3 proteins. -
细胞定位
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. - Information by UniProt
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数据库链接
- Entrez Gene: 12531 Mouse
- SwissProt: P30306 Mouse
- Unigene: 38444 Mouse
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别名
- AI604853 antibody
- Cdc 25B antibody
- Cdc25b antibody
see all
图片
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab53103 被引用在 3 文献中.
- Xu X et al. A signature motif in LIM proteins mediates binding to checkpoint proteins and increases tumour radiosensitivity. Nat Commun 8:14059 (2017). WB ; Human . PubMed: 28094252
- Zhao X et al. Successive recruitment of p-CDC25B-Ser351 and p-cyclin B1-Ser123 to centrosomes contributes to the release of mouse oocytes from prophase I arrest. Dev Dyn 244:110-21 (2015). PubMed: 25349079
- Shen W et al. Female infertility in PDE3A (-/-) mice: Polo-like kinase 1 (Plk1) may be a target of Protein Kinase A (PKA) and involved in meiotic arrest of oocytes from PDE3A (-/- ) mice. Cell Cycle 9:4720-34 (2010). WB ; Mouse . PubMed: 21099356