Anti-Cdc25A抗体[DCS-120 + DCS-121] (ab2357)

Reviews (1)Q&A (3)References (10)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc25A antibody [DCS-120 + DCS-121] (ab2357)

    Key features and details

    • Mouse monoclonal [DCS-120 + DCS-121] to Cdc25A
    • Suitable for: IHC-P
    • Reacts with: Rat, Human
    • Isotype: IgG2a

    概述

    • 产品名称

      Anti-Cdc25A抗体[DCS-120 + DCS-121]
      参阅全部 Cdc25A 一抗

    • 描述

      小鼠单克隆抗体[DCS-120 + DCS-121] to Cdc25A

    • 宿主

      Mouse

    • 经测试应用

      适用于: IHC-Pmore details

    • 种属反应性

      与反应: Rat, Human

    • 免疫原

      Recombinant full length protein (Human).

    • 阳性对照

      • Tonsil.

    • 常规说明

      Please note that this antibody is an oligoclonal antibody. It is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen. 

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    性能

    应用

    Our Abpromise guarantee covers the use of ab2357 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. ABC method.

    靶标

    • 功能

      Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.

    • 序列相似性

      Belongs to the MPI phosphatase family.
      Contains 1 rhodanese domain.

    • 结构域

      The phosphodegron motif mediates interaction with specific F-box proteins when phosphorylated. Putative phosphorylation sites at Ser-79 and Ser-82 appear to be essential for this interaction.

    • 翻译后修饰

      Phosphorylated by CHEK1 on Ser-76, Ser-124, Ser-178, Ser-279, Ser-293 and Thr-507 during checkpoint mediated cell cycle arrest. Also phosphorylated by CHEK2 on Ser-124, Ser-279, and Ser-293 during checkpoint mediated cell cycle arrest. Phosphorylation on Ser-178 and Thr-507 creates binding sites for YWHAE/14-3-3 epsilon which inhibits CDC25A. Phosphorylation on Ser-76, Ser-124, Ser-178, Ser-279 and Ser-293 may also promote ubiquitin-dependent proteolysis of CDC25A by the SCF complex. Phosphorylation of CDC25A at Ser-76 by CHEK1 primes it for subsequent phosphorylation at Ser-79, Ser-82 and Ser-88 by NEK11. Phosphorylation by NEK11 is required for BTRC-mediated polyubiquitination and degradation. Phosphorylation by PIM1 leads to an increase in phosphatase activity. Phosphorylated by PLK3 following DNA damage, leading to promote its ubiquitination and degradation.
      Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) ubiquitin ligase complex that contains FZR1/CDH1 during G1 phase leading to its degradation by the proteasome. Ubiquitinated by a SCF complex containing BTRC and FBXW11 during S phase leading to its degradation by the proteasome. Deubiquitination by USP17L2/DUB3 leads to its stabilization.
    • Information by UniProt

    • 数据库链接

      • 别名

        • Cdc 25a antibody
        • CDC25A antibody
        • CDC25A2 antibody
        • CDC25A2 CAG isoform antibody
        • Cell division cycle 25 homolog A (S. pombe) antibody
        • Cell division cycle 25A antibody
        • Cell division cycle 25A isoform a antibody
        • Cell division cycle 25A isoform b antibody
        • D9Ertd393e antibody
        • Dual specificity phosphatase Cdc25A antibody
        • M phase inducer phosphatase 1 antibody
        • M-phase inducer phosphatase 1 antibody
        • MGC115549 antibody
        • MPIP1_HUMAN antibody
        see all

      图片

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc25A antibody [DCS-120 + DCS-121] (ab2357)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdc25A antibody [DCS-120 + DCS-121] (ab2357)
        ab2357 staining human tonsil by IHC-P.

      文献 (10)

      发表研究结果有使用 ab2357？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab2357 被引用在 10 文献中.

      • Ma Y  et al. B7-H3 promotes the cell cycle-mediated chemoresistance of colorectal cancer cells by regulating CDC25A. J Cancer 11:2158-2170 (2020). PubMed: 32127943
      • Blancas S  et al. Protein Expression Analysis in Uterine Cervical Cancer for Potential Targets in Treatment. Pathol Oncol Res 25:493-501 (2019). PubMed: 29532409
      • Sun G  et al. miR-19a promotes vascular smooth muscle cell proliferation, migration and invasion through regulation of Ras homolog family member B. Int J Mol Med 44:1991-2002 (2019). PubMed: 31573047
      • Ding FN  et al. miR-122-5p modulates the radiosensitivity of cervical cancer cells by regulating cell division cycle 25A (CDC25A). FEBS Open Bio 9:1869-1879 (2019). PubMed: 31505105
      • Sims JK & Wade PA Mi-2/NuRD complex function is required for normal S phase progression and assembly of pericentric heterochromatin. Mol Biol Cell 22:3094-102 (2011). PubMed: 21737684
      • Liberal V  et al. Cyclin-dependent kinase subunit (Cks) 1 or Cks2 overexpression overrides the DNA damage response barrier triggered by activated oncoproteins. Proc Natl Acad Sci U S A : (2011). WB ; Human . PubMed: 21697511
      • Kasahara K  et al. 14-3-3gamma mediates Cdc25A proteolysis to block premature mitotic entry after DNA damage. EMBO J 29:2802-12 (2010). WB ; Human . PubMed: 20639859
      • Honaker Y & Piwnica-Worms H Casein kinase 1 functions as both penultimate and ultimate kinase in regulating Cdc25A destruction. Oncogene 29:3324-34 (2010). PubMed: 20348946
      • Leisser C  et al. Subcellular localisation of Cdc25A determines cell fate. Cell Death Differ 11:134 (2004). PubMed: 14685169
      • Leisser C  et al. Subcellular localisation of Cdc25A determines cell fate. Cell Death Differ 11:80-9 (2004). PubMed: 12970676

      客户评价及客户问答

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      1-4 of 4 Abreviews or Q&A

      Question

      Customer kindly called to inquire about Ab2357 in IHC-P as they were getting reduced signal and higher background with the newest lot.

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      Answer

      Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

      I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number 1070441.

      To check the status of the order please contact our Customer Service team and reference this number.

      Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

      I wish you the best of luck with your research.

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      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Cdc25A antibody [DCS-120 + DCS-121]

       Below Average
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Human Tissue sections (BT474 Xenografts)
      Specification
      BT474 Xenografts
      Fixative
      Formaldehyde
      Antigen retrieval step
      Heat mediated
      Permeabilization
      No
      Blocking step
      Normal Goat Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 21°C
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      Abcam user community

      Verified customer

      提交于 Mar 17 2010

      Question

      what is the concentration?

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      Answer

      The concentration is 100 ug/ml. If you have any additional questions, please contact us again.

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      Question

      ANTIBODY CODE ab2357 BATCH NUMBER 40683 DESCRIPTION OF THE PROBLEM No signal or weak signal SAMPLE Cell lysates from human tumour cell lines PRIMARY ANTIBODY CDC25a (DCS120 + DCS121) 1:1000 and 1:500 in milk left in frdge overnight, wash for 10 mins before putting in secondary SECONDARY ANTIBODY anti mouse from DAKO 1:1000 incubate for 1hour wash for 1hour DETECTION METHOD ECL from AMersham 5mls of each reagant, leave for approx 1 minute POSITIVE AND NEGATIVE CONTROLS USED none as have none avaliable to me. ANTIBODY STORAGE CONDITIONS 4 degrees C SAMPLE PREPARATION lysate + laemmli buffer to 30ul. Heated to 94 degress for 10minutes before loading AMOUNT OF PROTEIN LOADED up to 50ug ELECTROPHORESIS/GEL CONDITIONS 4-20% gel (invitrogen) TRANSFER AND BLOCKING CONDITIONS transfer for 1.5 hours at 160mAmps Blocking for at least 1 hour in 5% milk + PBS HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 5 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? dilution of Primary antibody ADDITIONAL NOTES I have used the same method, the same secondary and the same ECL on other westersn which have worked. This antibody has also failed to work on IHC of paraffin embedded sections at a dilution of 1:100

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      Answer

      Thank you for your enquiry and providing more details of your protocol. This antibody has been optimized for paraffin tissue sections. We would suggest using at 1:25, 1:50 for 30 min. Pretreatment with Citrate buffer. Use tonsil as positive control along with a standard ABC kit (signal enhancing step). We think you are diluting antibody too much. Start with lower dilution and then move up with higher dilutions. If you need anything further or any help then please let us know.

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