重组Anti-Cdc23抗体[EPR11828] (ab182003)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11828] to Cdc23
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Cdc23抗体[EPR11828]
参阅全部 Cdc23 一抗 -
描述
兔单克隆抗体[EPR11828] to Cdc23 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- HepG2, HeLa, K562 and Jurkat cell lysates; HeLa cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR11828 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab182003于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 69 kDa).
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ICC/IF |
1/100.
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说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 69 kDa). |
ICC/IF
1/100. |
靶标
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功能
Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. -
通路
Protein modification; protein ubiquitination. -
序列相似性
Belongs to the APC8/CDC23 family.
Contains 9 TPR repeats. -
翻译后修饰
Phosphorylated. Phosphorylation on Thr-562 occurs specifically during mitosis. - Information by UniProt
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数据库链接
- Entrez Gene: 8697 Human
- Entrez Gene: 52563 Mouse
- Entrez Gene: 291689 Rat
- Omim: 603462 Human
- SwissProt: Q9UJX2 Human
- SwissProt: Q8BGZ4 Mouse
- Unigene: 73625 Human
- Unigene: 196638 Mouse
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别名
- ANAPC8 antibody
- Anaphase promoting complex subunit 8 antibody
- Anaphase-promoting complex subunit 8 antibody
see all
图片
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Immunofluorescent analysis of acetone-fixed HeLa cells labeling Cdc23 with ab182003 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 dilution (Left panel). Counter stained with Dapi (Right panel).
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All lanes : Anti-Cdc23 antibody [EPR11828] (ab182003) at 1/10000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : K562 cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 69 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
实验方案
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (3)
ab182003 被引用在 3 文献中.
- Tischer T et al. The APC/C targets the Cep152-Cep63 complex at the centrosome to regulate mitotic spindle assembly. J Cell Sci 135:N/A (2022). PubMed: 34878135
- Yatskevich S et al. Molecular mechanisms of APC/C release from spindle assembly checkpoint inhibition by APC/C SUMOylation. Cell Rep 34:108929 (2021). PubMed: 33789095
- Achari C et al. Expression of miR-34c induces G2/M cell cycle arrest in breast cancer cells. BMC Cancer 14:538 (2014). WB ; Human . PubMed: 25064703