重组Anti-CD99抗体[EPR3096] (ab108297)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3096] to CD99
- Suitable for: WB, IP, IHC-P, Flow Cyt, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-CD99抗体[EPR3096]
参阅全部 CD99 一抗 -
描述
兔单克隆抗体[EPR3096] to CD99 -
宿主
Rabbit -
经测试应用
适用于: WB, IP, IHC-P, Flow Cyt, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: THP-1, Jurkat, U937, Molt-4, HuT-78, and HUVEC cell lysates. IHC: Human pancreas, Human tonsil and Human uterus tissues IP: HUVEC cells. ICC/IF: Jurkat cells. Flow Cyt: Molt-4 cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.31% Sodium citrate, 0.175% Sodium chloride, 0.0172% EDTA disodium salt, 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR3096 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab108297于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 28, 32 kDa (predicted molecular weight: 19 kDa).
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IP |
1/10 - 1/100.
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Perform antigen retrieval before commencing with IHC staining protocol. |
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Flow Cyt |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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ICC/IF |
1/250 - 1/500.
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说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 28, 32 kDa (predicted molecular weight: 19 kDa). |
IP
1/10 - 1/100. |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Perform antigen retrieval before commencing with IHC staining protocol. |
Flow Cyt
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ICC/IF
1/250 - 1/500. |
靶标
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功能
Involved in T-cell adhesion processes. It is involved in spontaneous rosette formation with erythrocytes. -
序列相似性
Belongs to the CD99 family. -
翻译后修饰
Extensively O-glycosylated. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 4267 Human
- Omim: 313470 Human
- SwissProt: P14209 Human
- Unigene: 653349 Human
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别名
- 12E7 antibody
- Antigen identified by monoclonal 12E7, Y homolog antibody
- Antigen identified by monoclonal antibodies 12E7, F21 and O13 antibody
see all
图片
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All lanes : Anti-CD99 antibody [EPR3096] (ab108297) at 1/1000 dilution
Lane 1 : THP-1 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : U937 cell lysate
Lane 4 : Molt-4 cell lysate
Lane 5 : HuT-78 cell lysate
Lane 6 : HUVEC cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 19 kDa
Observed band size: 28,32 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/Immunofluorescence analysis of Jurkat (Human T cell leukemia cell line from peripheral blood) labelling CD99 with purified ab108297 at 1/500. Cells were fixed with 4% PFA and permeabilized with 0.1% triton X-100. ab150077 Goat anti rabbit IgG (Alexa Fluor® 488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue using ab108297 at a dilution of 1/250.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Flow cytometric analysis of Molt-4 cells using anti-CD99 RabMAb (red) (ab108297) or a rabbit IgG (negative) (green).
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Fluorescent immunohistochemical analysis of paraffin-embedded human normal pancreas tissue using ab108297. Green-CD99 red-PI
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical staining of paraffin-embedded Human tonsil tissue using ab108297 at a dilution of 1/250.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical staining of paraffin-embedded Human uterus tissue using ab108297 at a dilution of 1/250.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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CD99 was immunoprecipitated from 0.35 mg HUVEC (Human umbilical vein endothelial cell) whole cell lysate 10 µg with 108297 at 1/50 dilution (2µg). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: HUVEC (Human umbilical vein endothelial cell) whole cell lysate 10 µg
Lane 2: ab108297 IP in HUVEC whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab108297 in HUVEC whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
CD99 homodimer was reported in literature. (PMID:18160845)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab108297 被引用在 5 文献中.
- Vasileva E et al. Dysregulated heparan sulfate proteoglycan metabolism promotes Ewing sarcoma tumor growth. Elife 11:N/A (2022). PubMed: 35285802
- Mao X et al. Superficial CD34-positive fibroblastic tumor: report of two cases and review of literature. Int J Clin Exp Pathol 13:38-43 (2020). PubMed: 32055270
- Wu J et al. Nrf2 induced cisplatin resistance in ovarian cancer by promoting CD99 expression. Biochem Biophys Res Commun 518:698-705 (2019). PubMed: 31472965
- Rao DD et al. Preclinical Justification of pbi-shRNA EWS/FLI1 Lipoplex (LPX) Treatment for Ewing's Sarcoma. Mol Ther 24:1412-22 (2016). PubMed: 27166877
- Weekes MP et al. Quantitative temporal viromics: an approach to investigate host-pathogen interaction. Cell 157:1460-72 (2014). WB ; Human . PubMed: 24906157