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Immunology Adaptive Immunity T Cells CD

Anti-CD90 / Thy1抗体[MRC OX-7] - Hematopoietic Stem Cell Marker (ab225)

  • Datasheet
  • SDS
Reviews (11)Q&A (5)References (72)

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Flow Cytometry - Anti-CD90 / Thy1 antibody [MRC OX-7] - Hematopoietic Stem Cell Marker (ab225)
  • Immunocytochemistry - Anti-CD90 / Thy1 antibody [MRC OX-7] - Hematopoietic Stem Cell Marker (ab225)
  • Immunocytochemistry - Anti-CD90 / Thy1 antibody [MRC OX-7] - Hematopoietic Stem Cell Marker (ab225)
  • Western blot - Anti-CD90 / Thy1 antibody [MRC OX-7] - Hematopoietic Stem Cell Marker (ab225)
  • Immunocytochemistry - Anti-CD90 / Thy1 antibody [MRC OX-7] - Hematopoietic Stem Cell Marker (ab225)
  • Immunocytochemistry - Anti-CD90 / Thy1 antibody [MRC OX-7] - Hematopoietic Stem Cell Marker (ab225)

Key features and details

  • Mouse monoclonal [MRC OX-7] to CD90 / Thy1 - Hematopoietic Stem Cell Marker
  • Suitable for: ICC, Flow Cyt, WB
  • Reacts with: Rat
  • Isotype: IgG1

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概述

  • 产品名称

    Anti-CD90 / Thy1抗体[MRC OX-7] - Hematopoietic Stem Cell Marker
    参阅全部 CD90 / Thy1 一抗
  • 描述

    小鼠单克隆抗体[MRC OX-7] to CD90 / Thy1 - Hematopoietic Stem Cell Marker
  • 宿主

    Mouse
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Rat
    ICC
    Rat
    WB
    Rat
    See all applications and species data
  • 免疫原

    Full length protein corresponding to Rat CD90/ Thy1.

  • 阳性对照

    • WB: rat brain tissue lysate and PC12 whole cell lysate. ICC: PC12 cells.
  • 常规说明

    This antibody clone is manufactured by Abcam.

    The affinity of the Fab' of MRC OX-7 for rat Thy-1 is 3 x 109m-1 and for mouse Thy-1.1 is 3 x 108m-1.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Some batches contain 6.97% L-Arginine as a stabilizing agent. For lot-specific buffer information, please contact our Scientific Support team.
  • Concentration information loading...
  • 纯度

    Protein G purified
  • 克隆

    单克隆
  • 克隆编号

    MRC OX-7
  • 骨髓瘤

    NS1
  • 同种型

    IgG1
  • 轻链类型

    kappa
  • 研究领域

    • Immunology
    • Adaptive Immunity
    • T Cells
    • CD
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Synapse marker
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • B Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • T Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Monocytic Lineage

相关产品

  • Alternative Versions

    • HRP Anti-CD90 / Thy1 antibody [MRC OX-7] (ab199219)
    • PerCP Anti-CD90 / Thy1 antibody [MRC OX-7] (ab216354)
    • Anti-CD90 / Thy1 antibody [MRC OX-7] - BSA and Azide free (ab222781)
    • FITC Anti-CD90 / Thy1 antibody [MRC OX-7] (ab226)
    • PE Anti-CD90 / Thy1 antibody [MRC OX-7] (ab33694)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant Human CD90 / Thy1 protein (Fc Chimera) (ab157072)

应用

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab225 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

应用 Species
Flow Cyt
Rat
ICC
Rat
WB
Rat
All applications
Mouse
Rabbit
Horse
Guinea pig
应用 Ab评论 说明
ICC (1)
Use at an assay dependent concentration.
Flow Cyt (1)
Use 0.1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

WB (2)
Use a concentration of 5 µg/ml. Detects a band of approximately 35-37 kDa (predicted molecular weight: 17 kDa).
说明
ICC
Use at an assay dependent concentration.
Flow Cyt
Use 0.1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

WB
Use a concentration of 5 µg/ml. Detects a band of approximately 35-37 kDa (predicted molecular weight: 17 kDa).

靶标

  • 功能

    May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.
  • 序列相似性

    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • 细胞定位

    Cell membrane.
  • Target information above from: UniProt accession P04216 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 21838 Mouse
    • Entrez Gene: 24832 Rat
    • SwissProt: P01831 Mouse
    • SwissProt: P01830 Rat
    • Unigene: 3951 Mouse
    • Unigene: 108198 Rat
    • 别名

      • CD7 antibody
      • CD90 antibody
      • CD90 antigen antibody
      • CDw90 antibody
      • FLJ33325 antibody
      • MGC128895 antibody
      • T25 antibody
      • Theta antigen antibody
      • Thy 1 antibody
      • Thy 1 cell surface antigen antibody
      • Thy 1 membrane glycoprotein antibody
      • Thy 1 T cell antigen antibody
      • Thy 1.2 antibody
      • Thy-1 antigen antibody
      • Thy-1 membrane glycoprotein antibody
      • Thy1 antibody
      • Thy1 antigen antibody
      • Thy1 T cell antigen antibody
      • Thy1.1 antibody
      • Thy1.2 antibody
      • THY1_HUMAN antibody
      • Thymus cell antigen 1, theta antibody
      see all

    图片

    • Flow Cytometry - Anti-CD90 / Thy1 antibody [MRC OX-7] - Hematopoietic Stem Cell Marker (ab225)
      Flow Cytometry - Anti-CD90 / Thy1 antibody [MRC OX-7] - Hematopoietic Stem Cell Marker (ab225)

      Overlay histogram showing PC12 cells stained with ab225 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab225, 0.1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [B11/6](ab91353, 1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive result in 80% methanol (5 min) fixed PC12 cells used under the same conditions.

    • Immunocytochemistry - Anti-CD90 / Thy1 antibody [MRC OX-7] - Hematopoietic Stem Cell Marker (ab225)
      Immunocytochemistry - Anti-CD90 / Thy1 antibody [MRC OX-7] - Hematopoietic Stem Cell Marker (ab225)This image is courtesy of an abreview submitted by Vega Villar, University of Leon.

      Immunocytochemistry/ Immunofluorescence analysis of horse adipose and bone marrow stem cells labeling CD90 / Thy1 with ab225 at 1/1000 dilution. Cells were fixed in paraformaldehyde and permeabilized with Triton x100 0.1%. The cells were blocked with 10% BSA for 30 minutes at 37°C, followed by staining with ab225 at 1/1000 for 12 hours in PBS+IGEPAL+BSA+10%NGS at 4°C. Goat F(ab')2 Anti-Mouse IgG - (Fab')2 (Biotin), pre-adsorbed (ab5886) was used as the secondary antibody at 1/400 dilution.

      See Abreview

    • Immunocytochemistry - Anti-CD90 / Thy1 antibody [MRC OX-7] - Hematopoietic Stem Cell Marker (ab225)
      Immunocytochemistry - Anti-CD90 / Thy1 antibody [MRC OX-7] - Hematopoietic Stem Cell Marker (ab225)This image is courtesy of an anonymous abreview.

      Immunocytochemistry/ Immunofluorescence analysis of rat sciatic nerve schwann cells and fibroblasts labeling CD90 / Thy1 with ab225 at 1/400 dilution. Cells were fixed in paraformaldehyde and permeabilized with 0.5% Triton X-100. The cells were blocked with 5% serum for 1 hour at 21°C, followed by staining with ab225 at 1/400 in 0.2% BSA for 1 hour at 37°C. A polyclonal goat anti-mouse Alexa Fluor® 546 secondary antibody was used at 1/500 dilution.

      See Abreview

    • Western blot - Anti-CD90 / Thy1 antibody [MRC OX-7] - Hematopoietic Stem Cell Marker (ab225)
      Western blot - Anti-CD90 / Thy1 antibody [MRC OX-7] - Hematopoietic Stem Cell Marker (ab225)
      All lanes : Anti-CD90 / Thy1 antibody [MRC OX-7] - Hematopoietic Stem Cell Marker (ab225) at 5 µg/ml

      Lane 1 : Brain (Rat) Tissue Lysate
      Lane 2 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/10000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 17 kDa
      Observed band size: 35-37 kDa why is the actual band size different from the predicted?


      Exposure time: 20 minutes


      Rat CD90/Thy1 is N-glycosylated at three sites, giving rise to molecules with a range of molecular masses (25-37 kDa). 

    • Immunocytochemistry - Anti-CD90 / Thy1 antibody [MRC OX-7] - Hematopoietic Stem Cell Marker (ab225)
      Immunocytochemistry - Anti-CD90 / Thy1 antibody [MRC OX-7] - Hematopoietic Stem Cell Marker (ab225)

      ab225 stained PC12 cells. The cells were 100% methanol fixed for 5 minutes at -20°C and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab225 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (ab150117) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

    • Immunocytochemistry - Anti-CD90 / Thy1 antibody [MRC OX-7] - Hematopoietic Stem Cell Marker (ab225)
      Immunocytochemistry - Anti-CD90 / Thy1 antibody [MRC OX-7] - Hematopoietic Stem Cell Marker (ab225)
      This image was kindly supplied as part of the review submitted by Nick Voilley. Rat retinal ganglion cell labelled with ab225 as a primary antibody and an anti-mouse F(ab)' 2 coupled to Alexa488 as a secondary antibody. The cell is approximately 15 micrometers in diameter. Only the cells labelled in green in the culture bear action potentials when stimulated. These three elements (reactivity to ab225, size and elecrophysiological parameters) clearly indicate the cell is a ganglion cell.

    实验方案

    • Flow cytometry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (72)

    发表研究结果有使用 ab225?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab225 被引用在 72 文献中.

    • Hasani Fard AH  et al. Investigation of cholestasis-related changes in characteristics of spermatogonial stem cells in testis tissue of male Wistar rats. Andrologia N/A:e13660 (2020). PubMed: 32478921
    • Li Z  et al. Biomechanically, structurally and functionally meticulously tailored polycaprolactone/silk fibroin scaffold for meniscus regeneration. Theranostics 10:5090-5106 (2020). PubMed: 32308770
    • Yang H  et al. Homeobox C8 inhibited the osteo-/dentinogenic differentiation and migration ability of stem cells of the apical papilla via activating KDM1A. J Cell Physiol N/A:N/A (2020). PubMed: 32246725
    • Gao L  et al. Vps35 Deficiency Impairs Cdk5/p35 Degradation and Promotes the Hyperphosphorylation of Tau Protein in Retinal Ganglion Cells. Invest Ophthalmol Vis Sci 61:1 (2020). PubMed: 31995153
    • Zhang S  et al. RSK-3 promotes cartilage regeneration via interacting with rpS6 in cartilage stem/progenitor cells. Theranostics 10:6915-6927 (2020). PubMed: 32550912
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-10 of 16 Abreviews or Q&A

    Western blot abreview for Anti-CD90 / Thy1 antibody [MRC OX-7] - Hematopoietic Stem Cell Marker

    Good
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (BRAIN)
    Gel Running Conditions
    Reduced Non-Denaturing (Native) (4-20% gradient)
    Loading amount
    30 µg
    Specification
    BRAIN
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More

    Abcam user community

    Verified customer

    提交于 Oct 27 2019

    Immunocytochemistry/ Immunofluorescence abreview for Anti-CD90 / Thy1 antibody [MRC OX-7] - Hematopoietic Stem Cell Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rabbit Cell (Primary bone marrow-derived stromal cells)
    Permeabilization
    No
    Specification
    Primary bone marrow-derived stromal cells
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More

    Dr. Egi Kardia

    Verified customer

    提交于 Sep 21 2018

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD90 / Thy1 antibody [MRC OX-7] - Hematopoietic Stem Cell Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rabbit Tissue sections (Bone and cartilage)
    Antigen retrieval step
    Other
    Permeabilization
    No
    Specification
    Bone and cartilage
    Blocking step
    BSA as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 21°C
    Fixative
    70 % Ethanol
    Read More

    Abcam user community

    Verified customer

    提交于 Aug 26 2014

    Flow Cytometry abreview for Anti-CD90 / Thy1 antibody [MRC OX-7] - Hematopoietic Stem Cell Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Mouse Cell (mMSC)
    Permeabilization
    No
    Specification
    mMSC
    Preparation
    Cell harvesting/tissue preparation method: bone marrow extraction
    Sample buffer: PBS
    Fixation
    Methanol
    Read More

    Abcam user community

    Verified customer

    提交于 Dec 19 2012

    Western blot abreview for Anti-CD90 / Thy1 antibody [MRC OX-7] - Hematopoietic Stem Cell Marker

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (mMSC)
    Gel Running Conditions
    Reduced Denaturing (10)
    Loading amount
    70 µg
    Specification
    mMSC
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    提交于 Dec 18 2012

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD90 / Thy1 antibody [MRC OX-7] - Hematopoietic Stem Cell Marker

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Pancreas)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate pH 6
    Permeabilization
    No
    Specification
    Pancreas
    Blocking step
    FBS / BSA in TBS as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More

    Mr. Raul Peña

    Verified customer

    提交于 Dec 03 2012

    Question

    Do cells need to be permeabilized before using this antibody in flow cytometry?

    Read More

    Abcam community

    Verified customer

    Asked on Aug 08 2012

    Answer

    Thank you very much for your call yesterday and for your patience.
    I have confirmed with the lab that the antibody is tested with non-permeabilized cells in flow cytometry. So, the epitope is extracellular.
    I hope that this information will be useful, but if you have any further questions please let me know and I'll be happy to help.

    Read More

    Abcam Scientific Support

    回复于 Aug 08 2012

    Question

    Dear technical support team:
    This customer has highly interested in ab225 (Anti-CD90 / Thy1 antibody [MRC OX-7]), he would likes to order this antibody and test in IHC-Fr with porcine sample, would you please help this customer to create a discount code, the information of this customer as follow:

    Thanks for your kindly help.
    Best regards

    Read More

    Abcam community

    Verified customer

    Asked on May 17 2012

    Answer

    Thank you for your enquiry and your interest.

    Unfortunately, we currently do not have any data (either empirical or from literature) that would support reactivity to porcine tissue. A quick BLAST comparison shows only a 75% homology between the rodent and porcine CD90.

    Based on this,we would expect only a weak reactivity to porcine tissue.

    Read More

    Abcam Scientific Support

    回复于 May 17 2012

    Question

    flow cytometry with human and rabbit mesenchymal stem cells
    problem: no signal
    fix: commercial solution
    ab11414: Ab dilution not sure
    ab225: 1/50
    no blocking step
    2nd: PE-conj., works well
    used controls - also no signal

    Read More

    Abcam community

    Verified customer

    Asked on May 11 2012

    Answer

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry these products did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx with ab11414andab225(different lots). The order will ship on Monday for delivery on Tuesday.

    To check the status of the order please contact our Customer Service team and reference this number.

    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

    I wish you the best of luck with your research.

    Read More

    Abcam Scientific Support

    回复于 May 11 2012

    Immunocytochemistry/ Immunofluorescence abreview for Anti-CD90 / Thy1 antibody [MRC OX-7] - Hematopoietic Stem Cell Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Horse Cell (Adipose and bone marrow stem cells)
    Permeabilization
    Yes - TRITON x100 0,1%
    Specification
    Adipose and bone marrow stem cells
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 37°C
    Fixative
    Paraformaldehyde
    Read More

    Ms. Vega Villar

    Verified customer

    提交于 Mar 29 2012

    1-10 of 16 Abreviews or Q&A

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