Anti-CD90 / Thy1抗体[MRC OX-7] (FITC) (ab226)

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概述

  • 产品名称

    Anti-CD90 / Thy1抗体[MRC OX-7] (FITC)
    参阅全部 CD90 / Thy1 一抗

  • 描述

    小鼠单克隆抗体[MRC OX-7] to CD90 / Thy1 (FITC)

  • 宿主

    Mouse

  • 偶联物

    FITC. Ex: 493nm, Em: 528nm

  • 特异性

    Recognises the Thy-1.1 antigenic determinant which is a monomorphic determinant within rat strains but polymorphic in mice. Thus MRC OX-7 reacts with Thy-1.1 mice eg. AKR strain, but not Thy-1.2 mice eg. CBA, BALB/c.

  • 经测试应用

    适用于: Flow Cyt, ICC/IFmore details

  • 种属反应性

    与反应: Mouse, Rat, Rabbit, Guinea pig

  • 免疫原

    Full length protein (Rat).

  • 阳性对照

    • FC: PC12 cells. ICC/IF: PC12 cells

  • 常规说明

    The affinity of the Fab' of MRC OX-7 for rat Thy-1 is 3 x 109m-1 and for mouse Thy-1.1 is 3 x 108m-1.

    Conjugated to Fluorescein Isothiocyanite Isomer 1 (FITC). FITC : Protein (molar ratio): 5.0 : 1.0.

性能

应用

Our Abpromise guarantee covers the use of ab226 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt 1/500.

ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 
ICC/IF 1/100.

This product gave a positive signal in PC12 cells fixed with 100% methanol (5 min)

靶标

  • 功能

    May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.

  • 序列相似性

    Contains 1 Ig-like V-type (immunoglobulin-like) domain.

  • 细胞定位

    Cell membrane.
  • Information by UniProt

  • 数据库链接

    • 别名

      • CD7 antibody
      • CD90 antibody
      • CD90 antigen antibody
      • CDw90 antibody
      • FLJ33325 antibody
      • MGC128895 antibody
      • T25 antibody
      • Theta antigen antibody
      • Thy 1 antibody
      • Thy 1 cell surface antigen antibody
      • Thy 1 membrane glycoprotein antibody
      • Thy 1 T cell antigen antibody
      • Thy 1.2 antibody
      • Thy-1 antigen antibody
      • Thy-1 membrane glycoprotein antibody
      • Thy1 antibody
      • Thy1 antigen antibody
      • Thy1 T cell antigen antibody
      • Thy1.1 antibody
      • Thy1.2 antibody
      • THY1_HUMAN antibody
      • Thymus cell antigen 1, theta antibody
      see all

    图片

    • Immunocytochemistry/ Immunofluorescence - Anti-CD90 / Thy1 antibody [MRC OX-7] (FITC) (ab226)
      Immunocytochemistry/ Immunofluorescence - Anti-CD90 / Thy1 antibody [MRC OX-7] (FITC) (ab226)

      ab226 staining CD90 / Thy1 in PC12 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab226 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • Flow Cytometry - Anti-CD90 / Thy1 antibody [MRC OX-7] (FITC) (ab226)
      Flow Cytometry - Anti-CD90 / Thy1 antibody [MRC OX-7] (FITC) (ab226)

      Overlay histogram showing PC12 cells stained with ab226 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab226, 1/500 dilution) for 30 min at 22°C.

      Isotype control antibody (black line) was Mouse IgG1 (monoclonal) FITC (ab18435) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

      Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.

    文献

    This product has been referenced in:

    • Sun AJ  et al. Comparison of mouse brown and white adipose-derived stem cell differentiation into pacemaker-like cells induced by TBX18 transduction. Mol Med Rep 17:7055-7064 (2018). Read more (PubMed: 29568953) »
    • Tarafder S  et al. Engineered Healing of Avascular Meniscus Tears by Stem Cell Recruitment. Sci Rep 8:8150 (2018). Read more (PubMed: 29802356) »
    See all 14 Publications for this product

    发表研究结果有使用 ab226？请让我们知道，以便我们可以引用本数据表中的参考文章。

    客户评价及客户问答

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    Question

    LOT NUMBER GR22543 ORDER NUMBER xxxxx DESCRIPTION OF THE PROBLEM Positive marker CD90 shows negative SAMPLE Rabbit mesenchymal stem cell PRIMARY ANTIBODY Ab226 1:100 dilution in FACS buffer(2%FBS(hear inactivated) in PBS(pH7.2) with 0.05%NaN3)for 30min-1hr DETECTION METHOD Analysis: FACS instrument BD FACS Cailbur FACS instrument is located in a core facility? Cells gated yes/no Yes POSITIVE AND NEGATIVE CONTROLS USED Positive control N/A Negative control IgG1 (Southern biotech, FITC, Cat. 0102-02) ANTIBODY STORAGE CONDITIONS -20C SAMPLE PREPARATION FACS buffer (2%FBS(hear inactivated) in PBS(pH7.2) with 0.05%NaN3) NUMBER OF CELLS USED 5X10[5] PERMEABILIZATION STEP N/A BLOCKING CONDITIONS 1% BSA in PBS 30-1 hr at 4C SECONDARY ANTIBODY N/A HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 3

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    Answer

    Thank you for contacting us and reporting the problems you have experienced in using ab226. The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. Having reviewed this case, I would like to offer some suggestions to help optimize the results from ab226. I would also appreciate if you can confirm some further details: 1. What washing steps were preformed? And with what buffers? 2. What volume of buffer was the cells suspended in for the primary antibody incubation? 3. Was the antibody kept in the dark at all times? 4. Was any analysis performed to detect the dead cells within the sample? I would suggest trying to use a higher concentration of primary antibody. The directions supplied with the datasheet are only guidelines and may need to be optimised for your particular experiment. I would suggest using a 1/10 dilution. It may also be worth trying a different blocking agent such as 3% BSA in PBS. Care should also be taken to keep the sample ice cold when preparing and I would suggest incubating the antibody with the cells (in the dark) for 1 hour at 4°C. Should the suggestions not improve the results, please do let me know. In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement, credit note, or refund. I hope this information is helpful, and I thank you for your cooperation.

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    Question

    Further to my email below, could you also tell me if the following antibody would bind the cell surface of NIH-3T3 cells?   https://www.abcam.com/Sca1-Ly6A-E-antibody-D7-FITC-ab25031.html#ab25031_1.jpg       Good afternoon,   I am looking for FITC and PE-conjugated antibodies to us as positive controls for flow cytometry compensation set-up. I am using NIH-3T3 cells and have found a publication suggesting cell-surface expression of Thy-1. Please could you confirm if the anti-CD90 mAb below would be suitable.   https://www.abcam.com/CD90-Thy1-antibody-FITC-MRC-OX-7-FITC-ab226.html#ab226_1.jpg           Many thanks in advance,        

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    Answer

    Thank you for contacting us. We are happy to help customers find the most suitable products for their research purposes; however we are not specialized in this particular field and do not want to recommend a product that may be unsuitable. We encourage customers to consult the latest literature available through PubMed and other resources in order to find the most up-to-date information about their specific research interests. I found conflicting information about the expression of CD90 on NIH3T3 cells: No expression or expression of Thy1.2 (CD90.2). ab226 only recognizes Thy1.1 (CD90.1). I therefore suggest to use ab25672 (Anti-CD90 / Thy1 antibody [G7] (FITC)) which will bind CD90.1 and CD90.2. I could not find any reliable data on the expression of Ly6 on NIH3T3 cells either, but can confirm that ab25031 will bind to Ly6A.2 and Ly6E. I am sorry that I could not be more helpful, but I hope that the available literature in this area can provide some clarification. Please do not hesitate to contact us again with other needs or with any questions about our products.

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