Anti-CD90 / Thy1抗体[MRC OX-7] (FITC) (ab226)

Thank you for contacting us and reporting the problems you have experienced in using ab226. The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. Having reviewed this case, I would like to offer some suggestions to help optimize the results from ab226. I would also appreciate if you can confirm some further details: 1. What washing steps were preformed? And with what buffers? 2. What volume of buffer was the cells suspended in for the primary antibody incubation? 3. Was the antibody kept in the dark at all times? 4. Was any analysis performed to detect the dead cells within the sample? I would suggest trying to use a higher concentration of primary antibody. The directions supplied with the datasheet are only guidelines and may need to be optimised for your particular experiment. I would suggest using a 1/10 dilution. It may also be worth trying a different blocking agent such as 3% BSA in PBS. Care should also be taken to keep the sample ice cold when preparing and I would suggest incubating the antibody with the cells (in the dark) for 1 hour at 4°C. Should the suggestions not improve the results, please do let me know. In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement, credit note, or refund. I hope this information is helpful, and I thank you for your cooperation.

Thank you for contacting us. We are happy to help customers find the most suitable products for their research purposes; however we are not specialized in this particular field and do not want to recommend a product that may be unsuitable. We encourage customers to consult the latest literature available through PubMed and other resources in order to find the most up-to-date information about their specific research interests. I found conflicting information about the expression of CD90 on NIH3T3 cells: No expression or expression of Thy1.2 (CD90.2). ab226 only recognizes Thy1.1 (CD90.1). I therefore suggest to use ab25672 (Anti-CD90 / Thy1 antibody [G7] (FITC)) which will bind CD90.1 and CD90.2. I could not find any reliable data on the expression of Ly6 on NIH3T3 cells either, but can confirm that ab25031 will bind to Ly6A.2 and Ly6E. I am sorry that I could not be more helpful, but I hope that the available literature in this area can provide some clarification. Please do not hesitate to contact us again with other needs or with any questions about our products.