Anti-CD90 / Thy1抗体[MRC OX-7] (FITC) (ab226)
概述
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产品名称
Anti-CD90 / Thy1抗体[MRC OX-7] (FITC)
参阅全部 CD90 / Thy1 一抗 -
描述
小鼠单克隆抗体[MRC OX-7] to CD90 / Thy1 (FITC) -
宿主
Mouse -
偶联物
FITC. Ex: 493nm, Em: 528nm -
特异性
Recognises the Thy-1.1 antigenic determinant which is a monomorphic determinant within rat strains but polymorphic in mice. Thus MRC OX-7 reacts with Thy-1.1 mice eg. AKR strain, but not Thy-1.2 mice eg. CBA, BALB/c. -
经测试应用
适用于: Flow Cyt, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Rabbit, Guinea pig -
免疫原
corresponding to CD90/ Thy1.
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阳性对照
- FC: PC12 cells. ICC/IF: PC12 cells
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常规说明
The affinity of the Fab' of MRC OX-7 for rat Thy-1 is 3 x 109m-1 and for mouse Thy-1.1 is 3 x 108m-1.
Conjugated to Fluorescein Isothiocyanite Isomer 1 (FITC). FITC : Protein (molar ratio): 5.0 : 1.0.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Store In the Dark. -
存储溶液
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol, 1% BSA -
Concentration information loading...
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纯度
Protein A purified -
纯化说明
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant -
克隆
单克隆 -
克隆编号
MRC OX-7 -
骨髓瘤
NS1 -
同种型
IgG1 -
轻链类型
unknown -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
Our Abpromise guarantee covers the use of ab226 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用 | Ab评论 | 说明 |
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Flow Cyt | 1/500. ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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ICC/IF | 1/100. This product gave a positive signal in PC12 cells fixed with 100% methanol (5 min) |
靶标
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功能
May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain. -
序列相似性
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 21838 Mouse
- Entrez Gene: 24832 Rat
- SwissProt: P01831 Mouse
- SwissProt: P01830 Rat
- Unigene: 3951 Mouse
- Unigene: 108198 Rat
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别名
- CD7 antibody
- CD90 antibody
- CD90 antigen antibody
see all
图片
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ab226 staining CD90 / Thy1 in PC12 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab226 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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Overlay histogram showing PC12 cells stained with ab226 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab226, 1/500 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was Mouse IgG1 (monoclonal) FITC (ab18435) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.
文献
This product has been referenced in:
- Sun AJ et al. Comparison of mouse brown and white adipose-derived stem cell differentiation into pacemaker-like cells induced by TBX18 transduction. Mol Med Rep 17:7055-7064 (2018). Read more (PubMed: 29568953) »
- Tarafder S et al. Engineered Healing of Avascular Meniscus Tears by Stem Cell Recruitment. Sci Rep 8:8150 (2018). Read more (PubMed: 29802356) »