Key features and details
- Mouse monoclonal [MRC OX-7] to CD90 / Thy1 (FITC)
- Suitable for: Flow Cyt, ICC/IF
- Reacts with: Mouse, Rat, Rabbit, Guinea pig
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG1
产品名称Anti-CD90 / Thy1抗体[MRC OX-7] (FITC)
参阅全部 CD90 / Thy1 一抗
描述小鼠单克隆抗体[MRC OX-7] to CD90 / Thy1 (FITC)
偶联物FITC. Ex: 493nm, Em: 528nm
特异性Recognises the Thy-1.1 antigenic determinant which is a monomorphic determinant within rat strains but polymorphic in mice. Thus MRC OX-7 reacts with Thy-1.1 mice eg. AKR strain, but not Thy-1.2 mice eg. CBA, BALB/c.
经测试应用适用于: Flow Cyt, ICC/IFmore details
种属反应性与反应: Mouse, Rat, Rabbit, Guinea pig
corresponding to CD90/ Thy1.
- Flow Cyt: PC12 cells. ICC/IF: PC12 cells
The affinity of the Fab' of MRC OX-7 for rat Thy-1 is 3 x 109m-1 and for mouse Thy-1.1 is 3 x 108m-1.
Conjugated to Fluorescein Isothiocyanite Isomer 1 (FITC). FITC : Protein (molar ratio): 5.0 : 1.0.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
存储溶液Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol, 1% BSA
Concentration information loading...
纯度Immunogen affinity purified
纯化说明Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Anti-CD90 / Thy1 antibody [MRC OX-7] (HRP) (ab199219)
- Anti-CD90 / Thy1 antibody [MRC OX-7] (PerCP) (ab216354)
- Anti-CD90 / Thy1 antibody [MRC OX-7] - BSA and Azide free (ab222781)
- Anti-CD90 / Thy1 antibody [MRC OX-7] - Hematopoietic Stem Cell Marker (ab225)
- Anti-CD90 / Thy1 antibody [MRC OX-7] (PE) (ab33694)
Our Abpromise guarantee covers the use of ab226 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
This product gave a positive signal in PC12 cells fixed with 100% methanol (5 min)
功能May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.
序列相似性Contains 1 Ig-like V-type (immunoglobulin-like) domain.
- Information by UniProt
- CD7 antibody
- CD90 antibody
- CD90 antigen antibody
ab226 staining CD90 / Thy1 in PC12 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab226 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Overlay histogram showing PC12 cells stained with ab226 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab226, 1/500 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was Mouse IgG1 (monoclonal) FITC (ab18435) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.
ab226 被引用在 16 文献中.
- Tarafder S et al. Engineered Healing of Avascular Meniscus Tears by Stem Cell Recruitment. Sci Rep 8:8150 (2018). PubMed: 29802356
- Pak S et al. Endoscopic Transplantation of Mesenchymal Stem Cell Sheets in Experimental Colitis in Rats. Sci Rep 8:11314 (2018). PubMed: 30054522
- Li J et al. Carbon monoxide releasing molecule-3 promotes the osteogenic differentiation of rat bone marrow mesenchymal stem cells by releasing carbon monoxide. Int J Mol Med 41:2297-2305 (2018). PubMed: 29393384
- Chopra H et al. Lapine periodontal ligament stem cells for musculoskeletal research in preclinical animal trials. J Transl Med 16:174 (2018). PubMed: 29929550
- Sun AJ et al. Comparison of mouse brown and white adipose-derived stem cell differentiation into pacemaker-like cells induced by TBX18 transduction. Mol Med Rep 17:7055-7064 (2018). PubMed: 29568953