Anti-CD9抗体[MZ3] (ab235119)
Key features and details
- Rat monoclonal [MZ3] to CD9
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Isotype: IgG2a
概述
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产品名称
Anti-CD9抗体[MZ3]
参阅全部 CD9 一抗 -
描述
大鼠单克隆抗体[MZ3] to CD9 -
宿主
Rat -
经测试应用
适用于: Flow Cytmore details -
种属反应性
与反应: Mouse -
免疫原
Tissue, cells or virus corresponding to CD9. Mouse B cells.
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阳性对照
- Flow Cyt: C57BL/6 bone marrow cells.
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
MZ3 -
同种型
IgG2a -
轻链类型
kappa -
研究领域
相关产品
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Alternative Versions
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab235119于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt |
Use a concentration of 0.04 µg/ml.
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说明 |
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Flow Cyt
Use a concentration of 0.04 µg/ml. |
靶标
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功能
Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion. -
组织特异性
Expressed by a variety of hematopoietic and epithelial cells. -
序列相似性
Belongs to the tetraspanin (TM4SF) family. -
翻译后修饰
Protein exists in three forms with molecular masses between 22 and 27 kDa, and is known to carry covalently linked fatty acids. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 12527 Mouse
- SwissProt: P40240 Mouse
- Unigene: 210676 Mouse
- Unigene: 404614 Mouse
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别名
- Tetraspanin 29 antibody
- 5H9 antibody
- 5H9 antigen antibody
see all
图片
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C57BL/6 mouse bone marrow cells stained with ab235119 (right) or Rat IgG2aκ (ab18450) isotype (left). C57BL/6 mouse bone marrow cells were incubated for 30 min on ice in PBS 10 % mouse serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab235119) or Rat IgG2aκ (ab18450) (1x106 in 100µl at 0.04 µg/ml) for 30 min on ice.
The secondary antibody Goat anti-rat IgG H&L (Alexa Fluor ® 488, pre-adsorbed) (ab150165) was used at 1/2000 dilution for 30 min at 4°C. The cells were simultaneously stained with CD11b antibody.
Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on viable single cells.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab235119 尚未被引用在任何文献中。