Anti-CD9抗体[MEM-61] (ab2215)
Key features and details
- Mouse monoclonal [MEM-61] to CD9
- Suitable for: IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-CD9抗体[MEM-61]
参阅全部 CD9 一抗 -
描述
小鼠单克隆抗体[MEM-61] to CD9 -
宿主
Mouse -
特异性
This antibody reacts with human CD9 antigen. -
Tested Applications & Species
Application Species Flow Cyt HumanIHC-P Human -
免疫原
Tissue/ cell preparation (Human). (Pre-B line Nalm-6).
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阳性对照
- Prostate IHC-P: Human lung FFPE tissue sections.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein A purified -
纯化说明
This product has been purified from tissue culture supernatant. Purity >95% by SDS-PAGE. -
克隆
单克隆 -
克隆编号
MEM-61 -
骨髓瘤
unknown -
同种型
IgG1 -
轻链类型
unknown -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab2215 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
应用 | Species |
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Flow Cyt |
Human
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IHC-P |
Human
|
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P | (1) |
Use a concentration of 20 µg/ml.
|
Flow Cyt |
Use a concentration of 1 - 4 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
说明 |
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IHC-P
Use a concentration of 20 µg/ml. |
Flow Cyt
Use a concentration of 1 - 4 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion. -
组织特异性
Expressed by a variety of hematopoietic and epithelial cells. -
序列相似性
Belongs to the tetraspanin (TM4SF) family. -
翻译后修饰
Protein exists in three forms with molecular masses between 22 and 27 kDa, and is known to carry covalently linked fatty acids. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 928 Human
- Omim: 143030 Human
- SwissProt: P21926 Human
- Unigene: 114286 Human
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别名
- Tetraspanin 29 antibody
- 5H9 antibody
- 5H9 antigen antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody [MEM-61] (ab2215)
IHC image of ab2215 staining in human lung formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2215, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Overlay histogram showing Jurkat cells stained with ab2215 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2215, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (26)
ab2215 被引用在 26 文献中.
- Chen HX et al. Exosomes derived from mesenchymal stem cells repair a Parkinson's disease model by inducing autophagy. Cell Death Dis 11:288 (2020). PubMed: 32341347
- Federici C et al. Natural-Killer-Derived Extracellular Vesicles: Immune Sensors and Interactors. Front Immunol 11:262 (2020). PubMed: 32231660
- Kim SE et al. Decursinol from Angelica gigas Nakai enhances endometrial receptivity during implantation. BMC Complement Med Ther 20:36 (2020). PubMed: 32024510
- Larios J et al. ALIX- and ESCRT-III-dependent sorting of tetraspanins to exosomes. J Cell Biol 219:N/A (2020). PubMed: 32049272
- Han F et al. Exosomal microRNA-26b-5p down-regulates ATF2 to enhance radiosensitivity of lung adenocarcinoma cells. J Cell Mol Med 24:7730-7742 (2020). PubMed: 32476275