Key features and details
- Mouse monoclonal [MEM-61] to CD9
- Suitable for: WB, IHC-P, Flow Cyt, Functional Studies
- Reacts with: Human
- Isotype: IgG1
参阅全部 CD9 一抗
描述小鼠单克隆抗体[MEM-61] to CD9
特异性This antibody reacts with human CD9 antigen.
经测试应用适用于: WB, IHC-P, Flow Cyt, Functional Studiesmore details
Tissue/ cell preparation (Human). (Pre-B line Nalm-6).
- Prostate IHC-P: Human lung FFPE tissue sections.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.097% Sodium azide
Concentration information loading...
纯度Protein A purified
纯化说明This product has been purified from tissue culture supernatant. Purity >95% by SDS-PAGE.
Our Abpromise guarantee covers the use of ab2215 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 2 - 4 µg/ml. Use under non reducing condition.|
|IHC-P||Use a concentration of 20 µg/ml.|
|Flow Cyt||Use a concentration of 5 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|Functional Studies||Use at an assay dependent concentration. The antibody induces FcgR-dependent platelet activation (aggregation).|
功能Involved in platelet activation and aggregation. Regulates paranodal junction formation. Involved in cell adhesion, cell motility and tumor metastasis. Required for sperm-egg fusion.
组织特异性Expressed by a variety of hematopoietic and epithelial cells.
序列相似性Belongs to the tetraspanin (TM4SF) family.
翻译后修饰Protein exists in three forms with molecular masses between 22 and 27 kDa, and is known to carry covalently linked fatty acids.
- Information by UniProt
- Tetraspanin 29 antibody
- 5H9 antibody
- 5H9 antigen antibody
IHC image of ab2215 staining in human lung formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2215, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Overlay histogram showing Jurkat cells stained with ab2215 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2215, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
ab2215 被引用在 26 文献中.
- Chen HX et al. Exosomes derived from mesenchymal stem cells repair a Parkinson's disease model by inducing autophagy. Cell Death Dis 11:288 (2020). PubMed: 32341347
- Federici C et al. Natural-Killer-Derived Extracellular Vesicles: Immune Sensors and Interactors. Front Immunol 11:262 (2020). PubMed: 32231660
- Kim SE et al. Decursinol from Angelica gigas Nakai enhances endometrial receptivity during implantation. BMC Complement Med Ther 20:36 (2020). PubMed: 32024510
- Larios J et al. ALIX- and ESCRT-III-dependent sorting of tetraspanins to exosomes. J Cell Biol 219:N/A (2020). PubMed: 32049272
- Han F et al. Exosomal microRNA-26b-5p down-regulates ATF2 to enhance radiosensitivity of lung adenocarcinoma cells. J Cell Mol Med 24:7730-7742 (2020). PubMed: 32476275