Anti-CD86抗体[BU63] - BSA and Azide free (ab213044)
Key features and details
- Mouse monoclonal [BU63] to CD86 - BSA and Azide free
- Suitable for: Flow Cyt, IHC-P
- Reacts with: Human
- Isotype: IgG1
Related conjugates and formulations
概述
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产品名称
Anti-CD86抗体[BU63] - BSA and Azide free
参阅全部 CD86 一抗 -
描述
小鼠单克隆抗体[BU63] to CD86 - BSA and Azide free -
宿主
Mouse -
经测试应用
适用于: Flow Cyt, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Tissue, cells or virus corresponding to Human CD86. ARH-77 (B-lymphoblastoid cell line).
Database link: P42081 -
阳性对照
- FACS: Human PBMC. IHC-P: Human esophageal tumor tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Constituent: 100% PBS -
无载体
是 -
Concentration information loading...
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纯度
Protein A/G purified -
纯化说明
Purified from bioreactor concentrate. -
克隆
单克隆 -
克隆编号
BU63 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Alternative Versions
- APC Anti-CD86 antibody [BU63], prediluted (ab134385)
- PE/Cy7® Anti-CD86 antibody [BU63] (ab233571)
- Anti-CD86 antibody [BU63] (ab234000)
- PE/Cy5® Anti-CD86 antibody [BU63] (ab234226)
- PerCP Anti-CD86 antibody [BU63] (ab77131)
- PE Anti-CD86 antibody [BU63] (ab77226)
- FITC Anti-CD86 antibody [BU63] (ab77276)
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab213044于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt |
Use 0.5-1µg for 106 cells.
Final volume 100 µL. |
|
IHC-P | (1) |
Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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说明 |
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Flow Cyt
Use 0.5-1µg for 106 cells. Final volume 100 µL. |
IHC-P
Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
靶标
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功能
Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation. -
组织特异性
Expressed by activated B-lymphocytes and monocytes. -
序列相似性
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
翻译后修饰
Polyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 942 Human
- Omim: 601020 Human
- SwissProt: P42081 Human
- Unigene: 171182 Human
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别名
- Activation B7-2 antigen 3 antibody
- Activation B7-2 antigen antibody
- B-lymphocyte activation antigen B7-2 2 antibody
see all
图片
实验方案
数据表及文件
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Datasheet download
文献 (22)
ab213044 被引用在 22 文献中.
- Fernandes PF et al. Immunoregulatory Effect of Preventive Supplementation of Omega-3 Fatty Acid in a Complex Regional Pain Syndrome Type I Model in Mice. Front Integr Neurosci 16:818692 (2022). PubMed: 35391753
- Zhang J et al. LncRNA HLA-F-AS1 promotes colorectal cancer metastasis by inducing PFN1 in colorectal cancer-derived extracellular vesicles and mediating macrophage polarization. Cancer Gene Ther 28:1269-1284 (2021). PubMed: 33531647
- Xu HZ et al. Synergy of nanodiamond-doxorubicin conjugates and PD-L1 blockade effectively turns tumor-associated macrophages against tumor cells. J Nanobiotechnology 19:268 (2021). PubMed: 34488792
- Kouroupis D et al. Single-Cell RNA-Sequencing Identifies Infrapatellar Fat Pad Macrophage Polarization in Acute Synovitis/Fat Pad Fibrosis and Cell Therapy. Bioengineering (Basel) 8:N/A (2021). PubMed: 34821732
- Yuanyuan L et al. Myeloid NEMO deficiency promotes tumor immunosuppression partly via MCP1-CCR2 axis. Exp Cell Res 399:112467 (2021). PubMed: 33428904