存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
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纯度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab66400 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 30 kDa).|
|IHC-P||1/50 - 1/80. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 22679510|
功能Associates with CD4 or CD8 and delivers costimulatory signals for the TCR/CD3 pathway.
序列相似性Belongs to the tetraspanin (TM4SF) family.
- Information by UniProt
- 4F9 antibody
- Antigen detected by monoclonal and antibody IA4 antibody
- C33 antibody
ab66400 (1:80) staining CD82 in paraffin-embedded human tonsil tissue (left panel) using an automated system (Ventana Discovery). Right hand panel shows negative control (no primary antibody).
Using this protocol there is strong membrane staining of follicular dendritic cells, and the FDC network forming the structure of germinal centres is clearly highlighted by this antibody. There is associated weak staining of smaller cells in the interfollicar areas which may be T cells.
Sections were rehydrated and antigen retrieved in CC1 Cell Conditioning Buffer using Ventana Standard Retrieval programme. Slides were blocked in 3% H2O2 / 4 min / 37°C and incubated with ab66400 (1:80 dilution / 1 hour / 37°C). Sections then blocked (4mins / 37°C) and incubated with Dako swine anti-rabbit antibody (1:50, 28 min / 37°C). Staining was amplified and detected by incubation with Ventana Streptavidin ABC system (16 min / 37°
Anti-CD82 antibody (ab66400) at 1 µg/ml + Human brain tissue lysate - total protein (ab29466) at 10 µg
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutes
CD82 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
ab66400 (1/50) staining CD82 in paraffin-embedded Mouse salivary gland tissue. Tissue underwent fixation in formaldehyde, peroxidase blocking, protein blocking and heat mediated antigen retrieval. The secondary antibody was goat anti rabbit/mouse conjugated to HRP. For further experimental details please refer to abreview.
Immunofluorescence analysis of HTR8/SVneo cell lines, staining CD82 (green) with ab66400. A FITC-conjugated anti-rabbit IgG (1/100) was used as the secondary antibody. Nuclei were detected by DAPI staining (blue).
This product has been referenced in:
- Wu J et al. Increased expression of microRNA-503 and reduced expression of kangai-1 in B-cell non-Hodgkin's lymphoma. Exp Ther Med 11:917-922 (2016). Read more (PubMed: 26998012) »
- Termini CM et al. The membrane scaffold CD82 regulates cell adhesion by altering a4 integrin stability and molecular density. Mol Biol Cell 25:1560-73 (2014). Read more (PubMed: 24623721) »