Anti-CD81抗体[1D6] (ab23505)
Key features and details
- Mouse monoclonal [1D6] to CD81
- Suitable for: Flow Cyt
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
Related conjugates and formulations
概述
-
产品名称
Anti-CD81抗体[1D6]
参阅全部 CD81 一抗 -
描述
小鼠单克隆抗体[1D6] to CD81 -
宿主
Mouse -
特异性
ab23505 recognises human CD81, a 26kD cell surface antigen also known as TAPA-1, and a member of the tetraspanin family. -
经测试应用
适用于: Flow Cytmore details
不适用于: WB -
种属反应性
与反应: Human
预测可用于: Sheep, Goat, Chimpanzee -
免疫原
Tissue, cells or virus corresponding to Human CD81. Aggregated OCI-LY8 cells (Human).
-
阳性对照
- Flow Cyt: HAP1-WT cells.
-
常规说明
This antibody induces homotypic adhesion and has powerful anti proliferative effects.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.40
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
-
纯度
Protein G purified -
Primary antibody说明
This antibody induces homotypic adhesion and has powerful anti proliferative effects. -
克隆
单克隆 -
克隆编号
1D6 -
骨髓瘤
P3x63-Ag8.653 -
同种型
IgG1 -
研究领域
相关产品
-
Alternative Versions
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
-
Recombinant Protein
-
Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab23505于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt |
1/5 - 1/10.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
说明 |
---|
Flow Cyt
1/5 - 1/10. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
-
功能
May play an important role in the regulation of lymphoma cell growth. Interacts with a 16-kDa Leu-13 protein to form a complex possibly involved in signal transduction. May acts a the viral receptor for HCV. -
组织特异性
Hematolymphoid, neuroectodermal and mesenchymal tumor cell lines. -
疾病相关
Defects in CD81 are the cause of immunodeficiency common variable type 6 (CVID6) [MIM:613496]; also called antibody deficiency due to CD81 defect. CVID6 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low. -
序列相似性
Belongs to the tetraspanin (TM4SF) family. -
翻译后修饰
Not glycosylated. -
细胞定位
Membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 449635 Chimpanzee
- Entrez Gene: 975 Human
- Omim: 186845 Human
- SwissProt: P60034 Chimpanzee
- SwissProt: P60033 Human
- Unigene: 54457 Human
-
别名
- 26 kDa cell surface protein TAPA 1 antibody
- 26 kDa cell surface protein TAPA-1 antibody
- 26 kDa cell surface protein TAPA1 antibody
see all
图片
-
Overlay histogram showing HAP1 wildtype (green line) and HAP1-CD81 knockout cells (red line) stained with ab23505. The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab23505, 0.1µg/ml) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) presorbed (ab150117) at 1/2000 dilution for 30 min at 22°C.
A mouse IgG1 isotype control antibody (ab170190) was used at the same concentration and conditions as the primary antibody (HAP1 wildtype - black line, HAP1-CD81 knockout - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).
Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.
This antibody can also be used in HAP1 cells fixed with 80% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 15 min under the same conditions.
实验方案
数据表及文件
-
Datasheet download
文献 (13)
ab23505 被引用在 13 文献中.
- Krishn SR et al. Small extracellular vesicle-mediated ITGB6 siRNA delivery downregulates the αVβ6 integrin and inhibits adhesion and migration of recipient prostate cancer cells. Cancer Biol Ther 23:173-185 (2022). PubMed: 35188070
- Naranjo NM et al. IFIT3 (interferon induced protein with tetratricopeptide repeats 3) modulates STAT1 expression in small extracellular vesicles. Biochem J 478:3905-3921 (2021). PubMed: 34622927
- Sayeed A et al. Profiling the circulating mRNA transcriptome in human liver disease. Oncotarget 11:2216-2232 (2020). PubMed: 32577166
- Krishn SR et al. The avß6 integrin in cancer cell-derived small extracellular vesicles enhances angiogenesis. J Extracell Vesicles 9:1763594 (2020). PubMed: 32595914
- Quaglia F et al. Small extracellular vesicles modulated by aVß3 integrin induce neuroendocrine differentiation in recipient cancer cells. J Extracell Vesicles 9:1761072 (2020). PubMed: 32922691