Anti-CD8抗体[MEM-31] (Allophycocyanin) (ab26004)

概述

  • 产品名称
    Anti-CD8抗体[MEM-31] (Allophycocyanin)
    参阅全部 CD8 一抗
  • 描述
    小鼠单克隆抗体[MEM-31] to CD8 (Allophycocyanin)
  • 宿主
    Mouse
  • 偶联物
    Allophycocyanin. Ex: 645nm, Em: 660nm
  • 经测试应用
    适用于: Flow Cytmore details
    不适用于: WB
  • 种属反应性
    与反应: Human
  • 免疫原

    Tissue/ cell preparation: Crude thymus membrane fraction. (Human).

  • 表位
    This antibody recognizes a conformationally dependent epitope of CD8 antigen.
  • 常规说明

    The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions.

性能

应用

Our Abpromise guarantee covers the use of ab26004 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use at an assay dependent concentration.

Use 5-20 µl per 100 µl of whole blood.

 

 

 

ab91364 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

  • 应用说明
    Is unsuitable for WB.
  • 靶标

    • 功能
      Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
    • 疾病相关
      Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
    • 序列相似性
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • 翻译后修饰
      All of the five most C-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not.
    • 细胞定位
      Secreted and Cell membrane.
    • Information by UniProt
    • 数据库链接
    • 形式
      CD8 beta tissue specificity: Isoform 1, isoform 3, isoform 5, isoform 6, isoform 7 and isoform 8 are expressed in both thymus and peripheral CD8+ T-cells. Expression of isoform 1 is higher in thymus CD8+ T-cells than in peripheral CD8+ T-cells. Expression of isoform 6 is higher in peripheral CD8+ T-cells than in thymus CD8+ T-cells. CD8 beta PTM: Phosphorylated as a consequence of T-cell activation.
    • 别名
      • CD8 antibody
      • CD8 antigen, alpha polypeptide (p32) antibody
      • CD8a antibody
      • CD8a antigen antibody
      • CD8a molecule antibody
      • CD8A_HUMAN antibody
      • Leu2 antibody
      • Leu2 T lymphocyte antigen antibody
      • MAL antibody
      • OKT8 T cell antigen antibody
      • p32 antibody
      • T cell antigen Leu2 antibody
      • T cell co receptor antibody
      • T-cell surface glycoprotein CD8 alpha chain antibody
      • T-lymphocyte differentiation antigen T8/Leu-2 antibody
      • T8 T cell antigen antibody
      see all

    文献

    This product has been referenced in:
    • Dieckmann NM  et al. Munc18-2 is required for Syntaxin 11 Localization on the Plasma Membrane in Cytotoxic T-Lymphocytes. Traffic 16:1330-41 (2015). Read more (PubMed: 26771955) »
    • Horejsí V  et al. Monoclonal antibodies against human leucocyte antigens. I. Antibodies against beta-2-microglobulin, immunoglobulin kappa light chains, HLA-DR-like antigens, T8 antigen, T1 antigen, a monocyte antigen, and a pan-leucocyte antigen. Folia Biol (Praha) 32:12-25 (1986). Read more (PubMed: 2422063) »
    See all 2 Publications for this product

    客户评价及客户问答

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    1-2 of 2 Abreviews

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Thymus)
    Specification
    Thymus
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate buffer PH6.0
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C

    Dr. R. Teng

    Verified customer

    提交于 May 03 2012

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Thymus)
    Specification
    Thymus
    Fixative
    Acetone
    Permeabilization
    Yes - Triton X-100
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C

    Dr. R. Teng

    Verified customer

    提交于 May 02 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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