APC Anti-CD8抗体[MEM-31] (ab26004)
Key features and details
- APC Mouse monoclonal [MEM-31] to CD8
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: APC. Ex: 645nm, Em: 660nm
- Isotype: IgG2a
概述
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产品名称
APC Anti-CD8抗体[MEM-31]
参阅全部 CD8 一抗 -
描述
APC小鼠单克隆抗体[MEM-31] to CD8 -
宿主
Mouse -
偶联物
APC. Ex: 645nm, Em: 660nm -
经测试应用
适用于: Flow Cytmore details
不适用于: WB -
种属反应性
与反应: Human -
免疫原
Tissue, cells or virus corresponding to Human CD8. Crude thymus membrane fraction
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表位
This antibody recognizes a conformationally dependent epitope of CD8 antigen. -
常规说明
The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.4
Preservative: 0.097% Sodium azide
Constituent: 0.2% BSA -
Concentration information loading...
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纯化说明
The conjugate is purified by size exclusion chromatography and adjusted for direct use. -
克隆
单克隆 -
克隆编号
MEM-31 -
同种型
IgG2a -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab26004于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt |
Use at an assay dependent concentration.
Use 5-20 ul per 100 ul of whole blood. ab91364 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
说明 |
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Flow Cyt
Use at an assay dependent concentration. Use 5-20 ul per 100 ul of whole blood. ab91364 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. -
疾病相关
Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections. -
序列相似性
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
翻译后修饰
All of the five most C-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not. -
细胞定位
Secreted and Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 925 Human
- Omim: 186910 Human
- SwissProt: P01732 Human
- Unigene: 85258 Human
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形式
CD8 beta tissue specificity: Isoform 1, isoform 3, isoform 5, isoform 6, isoform 7 and isoform 8 are expressed in both thymus and peripheral CD8+ T-cells. Expression of isoform 1 is higher in thymus CD8+ T-cells than in peripheral CD8+ T-cells. Expression of isoform 6 is higher in peripheral CD8+ T-cells than in thymus CD8+ T-cells. CD8 beta PTM: Phosphorylated as a consequence of T-cell activation. -
别名
- CD8 antibody
- CD8 antigen, alpha polypeptide (p32) antibody
- CD8a antibody
see all
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab26004 被引用在 3 文献中.
- Cui C et al. Propofol maintains Th17/Treg cell balance in elderly patients undergoing lung cancer surgery through GABAA receptor. BMC Immunol 23:58 (2022). PubMed: 36434505
- Dieckmann NM et al. Munc18-2 is required for Syntaxin 11 Localization on the Plasma Membrane in Cytotoxic T-Lymphocytes. Traffic 16:1330-41 (2015). PubMed: 26771955
- Horejsí V et al. Monoclonal antibodies against human leucocyte antigens. I. Antibodies against beta-2-microglobulin, immunoglobulin kappa light chains, HLA-DR-like antigens, T8 antigen, T1 antigen, a monocyte antigen, and a pan-leucocyte antigen. Folia Biol (Praha) 32:12-25 (1986). PubMed: 2422063