Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [CB3-1] to CD79b
- Suitable for: Flow Cyt
- Reacts with: Human
参阅全部 CD79b 一抗
描述小鼠单克隆抗体[CB3-1] to CD79b
经测试应用适用于: Flow Cytmore details
Fraction of Ig-associated molecules isolated from Ramos B cells.
- Flow Cyt: Human peripheral blood; Ramos cells.
This product has switched from a hybridoma to recombinant production method on 27th October 2020.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
纯度Protein A purified
Our Abpromise guarantee covers the use of ab130422 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
功能Required in cooperation with CD79A for initiation of the signal transduction cascade activated by the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. Enhances phosphorylation of CD79A, possibly by recruiting kinases which phosphorylate CD79A or by recruiting proteins which bind to CD79A and protect it from dephosphorylation.
疾病相关Defects in CD79B are the cause of agammaglobulinemia type 6 (AGM6) [MIM:612692]. It is a primary immunodeficiency characterized by profoundly low or absent serum antibodies and low or absent circulating B cells due to an early block of B-cell development. Affected individuals develop severe infections in the first years of life.
序列相似性Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Contains 1 ITAM domain.
翻译后修饰Phosphorylated on tyrosine upon B-cell activation.
细胞定位Cell membrane. Following antigen binding, the BCR has been shown to translocate from detergent-soluble regions of the cell membrane to lipid rafts although signal transduction through the complex can also occur outside lipid rafts.
- Information by UniProt
- AGM6 antibody
- B cell antigen receptor complex associated protein beta chain antibody
- B cell specific glycoprotein B29 antibody
Flow cytometric analysis of Ramos (human Burkitt's lymphoma cell line) cells labeling CD79b with ab130422 at 1/50 (10 μg/mL) dilution (Red) compared with an Isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Fc receptors were blocked with 20 μg/mL Human IgG for 15 minutes on ice. Primary antibody staining for 30 minutes on ice. Secondary antibody staining for 30 minutes on ice.
Flow cytometry staining of human PBMC for CD79b with ab130422 at 1/50 dilution (10 μg/mL) (right) or an isotype control (left). Fc receptors were blocked with 20 μg/mL Human IgG for 15 minutes on ice. Primary antibody staining for 30 minutes on ice. Secondary antibody staining for 30 minutes on ice.
ab130422 被引用在 2 文献中.
- Matutes E New additions to antibody panels in the characterisation of chronic lymphoproliferative disorders. J Clin Pathol 55:180-3 (2002). PubMed: 11896067
- Nakamura T et al. Heterogeneity of immunoglobulin-associated molecules on human B cells identified by monoclonal antibodies. Proc Natl Acad Sci U S A 89:8522-6 (1992). PubMed: 1382292