重组

重组Anti-CD79b抗体[CB3-1] (ab130422)

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Flow Cytometry - Anti-CD79b antibody [CB3-1] (ab130422)
  • Flow Cytometry - Anti-CD79b antibody [CB3-1] (ab130422)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [CB3-1] to CD79b
  • Suitable for: Flow Cyt
  • Reacts with: Human

概述

  • 产品名称

    Anti-CD79b抗体[CB3-1]
    参阅全部 CD79b 一抗

  • 描述

    小鼠单克隆抗体[CB3-1] to CD79b

  • 宿主

    Mouse

  • 经测试应用

    适用于: Flow Cytmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Fraction of Ig-associated molecules isolated from Ramos B cells.

  • 阳性对照

    • Flow Cyt: Human peripheral blood; Ramos cells.

  • 常规说明

    This product has switched from a hybridoma to recombinant production method on 27th October 2020.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

  • 存储溶液

    pH: 7.40
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 克隆

    单克隆

  • 克隆编号

    CB3-1

  • 同种型

    IgG1

  • 研究领域

应用

Our Abpromise guarantee covers the use of ab130422 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt 1/50.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能

    Required in cooperation with CD79A for initiation of the signal transduction cascade activated by the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. Enhances phosphorylation of CD79A, possibly by recruiting kinases which phosphorylate CD79A or by recruiting proteins which bind to CD79A and protect it from dephosphorylation.

  • 组织特异性

    B-cells.

  • 疾病相关

    Defects in CD79B are the cause of agammaglobulinemia type 6 (AGM6) [MIM:612692]. It is a primary immunodeficiency characterized by profoundly low or absent serum antibodies and low or absent circulating B cells due to an early block of B-cell development. Affected individuals develop severe infections in the first years of life.

  • 序列相似性

    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    Contains 1 ITAM domain.

  • 翻译后修饰

    Phosphorylated on tyrosine upon B-cell activation.

  • 细胞定位

    Cell membrane. Following antigen binding, the BCR has been shown to translocate from detergent-soluble regions of the cell membrane to lipid rafts although signal transduction through the complex can also occur outside lipid rafts.
  • Information by UniProt

  • 数据库链接

    • 别名

      • AGM6 antibody
      • B cell antigen receptor complex associated protein beta chain antibody
      • B cell specific glycoprotein B29 antibody
      • B-cell antigen receptor complex-associated protein beta chain antibody
      • B-cell-specific glycoprotein B29 antibody
      • B29 antibody
      • B29/Ig-beta/CD79b antibody
      • CD 79b antibody
      • CD79b antibody
      • CD79b antigen (immunoglobulin associated beta) antibody
      • CD79b antigen antibody
      • CD79b molecule antibody
      • CD79b molecule immunoglobulin associated beta antibody
      • CD79b protein antibody
      • CD79B_HUMAN antibody
      • Ig beta antibody
      • Ig-beta antibody
      • IGB antibody
      • Igbeta antibody
      • Immunoglobulin associated B29 antibody
      • Immunoglobulin associated B29 protein antibody
      • Immunoglobulin associated beta antibody
      • Immunoglobulin associated protein antibody
      • Immunoglobulin-associated B29 protein antibody
      • MGC108607 antibody
      see all

    图片

    • Flow Cytometry - Anti-CD79b antibody [CB3-1] (ab130422)
      Flow Cytometry - Anti-CD79b antibody [CB3-1] (ab130422)

      Flow cytometric analysis of Ramos (human Burkitt's lymphoma cell line) cells labeling CD79b with ab130422 at 1/50 (10 μg/mL) dilution (Red) compared with an Isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Fc receptors were blocked with 20 μg/mL Human IgG for 15 minutes on ice. Primary antibody staining for 30 minutes on ice. Secondary antibody staining for 30 minutes on ice.

    • Flow Cytometry - Anti-CD79b antibody [CB3-1] (ab130422)
      Flow Cytometry - Anti-CD79b antibody [CB3-1] (ab130422)

      Flow cytometry staining of human PBMC for CD79b with ab130422 at 1/50 dilution (10 μg/mL) (right) or an isotype control (left). Fc receptors were blocked with 20 μg/mL Human IgG for 15 minutes on ice. Primary antibody staining for 30 minutes on ice. Secondary antibody staining for 30 minutes on ice. 

    文献 (2)

    发表研究结果有使用 ab130422？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab130422 被引用在 2 文献中.

    • Matutes E New additions to antibody panels in the characterisation of chronic lymphoproliferative disorders. J Clin Pathol 55:180-3 (2002). PubMed: 11896067
    • Nakamura T  et al. Heterogeneity of immunoglobulin-associated molecules on human B cells identified by monoclonal antibodies. Proc Natl Acad Sci U S A 89:8522-6 (1992). PubMed: 1382292

    客户评价及客户问答

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    Question

    We are interested in the anti-CD79b [CB3-1] (code: ab130422) antibody, but were hoping you would have a picture of either a western blot or a flow stain for us to look at before we purchase anything? This would be a great help.

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    Answer

    on the left axis, there is side scatter signal (linear scale), on the bottom axis there is the fluorescence intensity (log scale). Human unstimulated blood was used, primary antibody concentration 1.6 ug/ml, secondary antibody Goat anti-mouse PE conjugate, Jackson Immunoresearch, red cell lysing solution was from An der Grub. The positive population are B lymphocytes, the negative populations are mainly T lymphocytes, monocytes and granulocytes. Following protocol was used:


    - Antibody titration: Dilute antibody in PBS+0,2% BSA to recommended concentration

    - Add 50μl of heparin-treated blood under carefull mixing

    - Incubate 20-30 min., dark, RT

    - Add 100µl of lysing solution and incubate 10min., RT (or according to the lysing solution manufacturers instructions).

    - Add 4 ml of deionized water to each tube, incubate 10 min., RT.

    - Centrifugate (1250 RPM at Hettich Universal 320R) 5 min, RT.

    - Suspend the pellet in 4ml PBS+0,2% BSA.

    - Centrifugate (1250 RPM at Hettich Universal 320R) 5 min, RT.

    - Suspend the pellet in 50 µl of secondary antibody (for dilultion follow manufacturers instruction)

    - Incubate 20-30 min., dark, RT

    - Add 4 ml of PBS+0,2% BSA

    - Centrifugate (1250 RPM at Hettich Universal 320R) 5 min, RT.

    - Suspend the pellet (almost unvisible) in 0,5 ml PBS+0,2% BSA

    - Store in cooler, measure within 24 hours on FACS







    Currently we do not have any WB image for this product.

    I hope this is helpful. Please contact me again if you have any further questions.

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    Question

    We are interested in the anti-CD79b [CB3-1] (code: ab130422) antibody, but were hoping you would have a picture of either a western blot or a flow stain for us to look at before we purchase anything? This would be a great help.

    Read More
    Answer

    I am sending flow cytometry data done on human peripheral blood cells. Unfortunately I was not able to find western blotting data.

    I hope this is helpful. Please contact me again if you have any further questions.

    Read More

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