Key features and details
- Rabbit polyclonal to CD79a
- Suitable for: IHC-P, ELISA, WB
- Reacts with: Human
- Isotype: IgG
存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA
Concentration information loading...
纯度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab5691 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ELISA||Use a concentration of 0.1 - 1 µg/ml.|
|WB||Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 25 kDa.|
功能Required in cooperation with CD79B for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. Also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells. Stimulates SYK autophosphorylation and activation. Binds to BLNK, bringing BLNK into proximity with SYK and allowing SYK to phosphorylate BLNK. Also interacts with and increases activity of some Src-family tyrosine kinases. Represses BCR signaling during development of immature B cells.
疾病相关Defects in CD79A are the cause of agammaglobulinemia type 3 (AGM3) [MIM:613501]. It is a primary immunodeficiency characterized by profoundly low or absent serum antibodies and low or absent circulating B cells due to an early block of B-cell development. Affected individuals develop severe infections in the first years of life. Note=Two different mutations, one at the splice donor site of intron 2 and the other at the splice acceptor site for exon 3, have been identified. Both mutations give rise to a truncated protein.
序列相似性Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 ITAM domain.
翻译后修饰Phosphorylated on tyrosine, serine and threonine residues upon B-cell activation. Phosphorylation of tyrosine residues by Src-family kinases is an early and essential feature of the BCR signaling cascade. The phosphorylated tyrosines serve as docking sites for SH2-domain containing kinases, leading to their activation which in turn leads to phosphorylation of downstream targets. Phosphorylation of serine and threonine residues may prevent subsequent tyrosine phosphorylation.
细胞定位Cell membrane. Following antigen binding, the BCR has been shown to translocate from detergent-soluble regions of the cell membrane to lipid rafts although signal transduction through the complex can also occur outside lipid rafts.
- Information by UniProt
- B lymphocyte-specific MB1 protein antibody
- B-cell antigen receptor complex-associated protein alpha chain antibody
- CD 79a antibody
ab5691 被引用在 2 文献中.
- Lopez D et al. Mapping hematopoiesis in a fully regenerative vertebrate: the axolotl. Blood 124:1232-41 (2014). PubMed: 24802774
- Cuttica MJ et al. Perivascular T-cell infiltration leads to sustained pulmonary artery remodeling after endothelial cell damage. Am J Respir Cell Mol Biol 45:62-71 (2011). IHC-P ; Mouse . PubMed: 20813993