Anti-CD73抗体[NT5E/2545] (ab257311)
Key features and details
- Mouse monoclonal [NT5E/2545] to CD73
- Suitable for: WB, Flow Cyt, ICC/IF, IHC-P, Protein Array
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-CD73抗体[NT5E/2545]
参阅全部 CD73 一抗 -
描述
小鼠单克隆抗体[NT5E/2545] to CD73 -
宿主
Mouse -
经测试应用
适用于: WB, Flow Cyt, ICC/IF, IHC-P, Protein Arraymore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein corresponding to Human CD73.
Database link: P21589 -
阳性对照
- IHC-P: Human tonsil, prostate carcinoma, pancreatic carcinoma and colon carcinoma tissue. Flow Cyt: U-87 MG cells. ICC/IF: U-87 MG cells. WB: A431 and PC3 cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading...
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纯度
Protein A/G purified -
纯化说明
Purified from bioreactor concentrate. -
克隆
单克隆 -
克隆编号
NT5E/2545 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab257311于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 71 kDa (predicted molecular weight: 63 kDa).
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Flow Cyt |
Use 1-2µg for 106 cells.
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ICC/IF |
Use a concentration of 1 - 2 µg/ml.
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IHC-P |
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Protein Array |
Use at an assay dependent concentration.
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说明 |
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WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 71 kDa (predicted molecular weight: 63 kDa). |
Flow Cyt
Use 1-2µg for 106 cells. |
ICC/IF
Use a concentration of 1 - 2 µg/ml. |
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Protein Array
Use at an assay dependent concentration. |
靶标
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功能
Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. -
疾病相关
Defects in NT5E are the cause of calcification of joints and arteries (CAJA) [MIM:211800]. A condition characterized by adult-onset calcification of the lower extremity arteries, including the iliac, femoral and tibial arteries, and hand and foot capsule joints. Age of onset has been reported as early as the second decade of life, usually involving intense joint pain or calcification in the hands. -
序列相似性
Belongs to the 5'-nucleotidase family. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 4907 Human
- Omim: 129190 Human
- SwissProt: P21589 Human
- Unigene: 153952 Human
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别名
- 5' NT antibody
- 5' nucleotidase (CD73) antibody
- 5' nucleotidase precursor antibody
see all
图片
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Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD73 using ab257311 at 2 μg/ml in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD73 using ab257311 at 2 μg/ml in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human prostate tissue stained for CD73 using ab257311 at 2 μg/ml in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human pancreatic carcinoma tissue stained for CD73 using ab257311 at 2 μg/ml in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for CD73 using ab257311 at 2 μg/ml in immunohistochemical analysis.
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Flow cytometric analysis of U-87 MG (human glioblastoma-astrocytoma epithelial cell line) cells labeling CD73 with ab257311 at 2 μg/ml followed by a goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
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Immunofluorescence staining of U-87 MG (human glioblastoma-astrocytoma epithelial cell line) cells using ab257311 at 2µg/ml followed by a goat anti-Mouse IgG conjugated to CF488 (green). Membrane stained with Phalloidin (Red).
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All lanes : Anti-CD73 antibody [NT5E/2545] (ab257311) at 2 µg/ml
Lane 1 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 2 : PC-3 (human prostate adenocarcinoma cell line) whole cell lysate
Predicted band size: 63 kDa -
Analysis of Protein Array containing more than 19,000 full-length human proteins using ab257311.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
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