Anti-CD6/T12抗体[3F7B5] - BSA and Azide free (ab213007)
Key features and details
- Mouse monoclonal [3F7B5] to CD6/T12 - BSA and Azide free
- Suitable for: Flow Cyt, IHC-P, ICC
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-CD6/T12抗体[3F7B5] - BSA and Azide free
参阅全部 CD6/T12 一抗 -
描述
小鼠单克隆抗体[3F7B5] to CD6/T12 - BSA and Azide free -
宿主
Mouse -
经测试应用
适用于: Flow Cyt, IHC-P, ICCmore details -
种属反应性
与反应: Human -
免疫原
Tissue, cells or virus corresponding to Human CD6/T12. Human rheumatoid synovial T cell line ST-1.
Database link: P30203 -
阳性对照
- IHC-P: Human tonsil tissue; ICC and Flow Cyt: MOLT-4 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Constituent: 100% PBS -
无载体
是 -
Concentration information loading...
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纯度
Protein G purified -
纯化说明
ab213007 was purified from Bioreactor Concentrate by Protein A/G. -
克隆
单克隆 -
克隆编号
3F7B5 -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
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Conjugation kits
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab213007于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt |
Use 1-2µg for 106 cells.
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IHC-P |
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Primary incubation for 30 minutes at room temperature. |
|
ICC |
Use a concentration of 1 - 2 µg/ml.
|
说明 |
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Flow Cyt
Use 1-2µg for 106 cells. |
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Primary incubation for 30 minutes at room temperature. |
ICC
Use a concentration of 1 - 2 µg/ml. |
靶标
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功能
Involved in cell adhesion. Binds to CD166. -
组织特异性
Expressed by thymocytes, mature T-cells, a subset of B-cells known as B-1 cells, and by some cells in the brain. -
序列相似性
Contains 3 SRCR domains. -
翻译后修饰
After T-cell activation, becomes hyperphosphorylated on Ser and Thr residues and phosphorylated on Tyr residues.
Contains intrachain disulfide bond(s). -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 923 Human
- Omim: 186720 Human
- SwissProt: P30203 Human
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别名
- CD_antigen=CD6 antibody
- CD6 antibody
- CD6 antigen Tp120 antibody
see all
图片
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Immunofluorescence staining of MOLT-4 cells labeling CD6/T12 with ab213007 followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot.
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Immunohistochemical analysis of formalix-fixed, paraffin-embedded Human tonsil tissue, labeling CD6/T12 using ab213007 at 1 µg/mL.
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This image was produced using ab193341, the same clone but in a different formulation. Flow Cytometric analysis of MOLT-4 (human lymphoblastic leukemia cell line) cells staining CD6/T12 using ab213007 at 2ug/million cells, followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).
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Immunohistochemical analysis of formalix-fixed, paraffin-embedded Human tonsil tissue, labeling CD6/T12 using ab213007 at 1 µg/mL.
数据表及文件
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Datasheet download
文献 (0)
ab213007 尚未被引用在任何文献中。