Key features and details
- Mouse monoclonal [PG-M1] to CD68, prediluted
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG3
参阅全部 CD68 一抗
描述小鼠单克隆抗体[PG-M1] to CD68，prediluted
特异性This antibody reacts with a glycoprotein of 110 kDa, expressed as intracytoplasmic molecule. It stains macrophages in a wide variety of tissues. Myeloid precursor cells and peripheral granulocytes are negative. This antibody does not react with granulocytes and their precursor cells.
经测试应用适用于: IHC-Pmore details
BALB/C mice were immunized with Gaucher’s cells.
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存放说明Shipped at 4°C. Store at +4°C. Do Not Freeze.
存储溶液Preservative: 0.05% Sodium azide
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Our Abpromise guarantee covers the use of ab844 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
No further titration is required.
We suggest an incubation period of 30-60 minutes at room temperature.
However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user.
Enzymatic predigestion with proteolytic enzymes is recommended for paraffin embedded tissue sections.
This antibody is not recommended for frozen sections or cell smears.
功能Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
组织特异性Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites.
序列相似性Belongs to the LAMP family.
翻译后修饰N- and O-glycosylated.
细胞定位Cell membrane and Endosome membrane. Lysosome membrane.
- Information by UniProt
- CD 68 antibody
- CD68 antibody
- CD68 antigen antibody
ab844 被引用在 1 文献中.
- Tewari A et al. Upregulation of MMP12 and its activity by UVA1 in human skin: potential implications for photoaging. J Invest Dermatol 134:2598-609 (2014). IF . PubMed: 24714202