Anti-CD68抗体[PG-M1]，prediluted (ab844)
概述
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产品名称Anti-CD68抗体[PG-M1]，prediluted
参阅全部 CD68 一抗 -
描述小鼠单克隆抗体[PG-M1] to CD68，prediluted
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宿主Mouse
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特异性This antibody reacts with a glycoprotein of 110 kDa, expressed as intracytoplasmic molecule. It stains macrophages in a wide variety of tissues. Myeloid precursor cells and peripheral granulocytes are negative. This antibody does not react with granulocytes and their precursor cells.
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经测试应用适用于: IHC-Pmore details
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种属反应性与反应: Human
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免疫原
BALB/C mice were immunized with Gaucher’s cells.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C. Do Not Freeze.
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存储溶液Preservative: 0.05% Sodium azide
Inert stabilizer -
Concentration information loading... -
克隆单克隆
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克隆编号PG-M1
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骨髓瘤unknown
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同种型IgG3
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轻链类型kappa
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研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
Our Abpromise guarantee covers the use of ab844 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
应用说明
This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections.
No further titration is required.
We suggest an incubation period of 30-60 minutes at room temperature.
However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user.
Enzymatic predigestion with proteolytic enzymes is recommended for paraffin embedded tissue sections.
This antibody is not recommended for frozen sections or cell smears.
No further titration is required.
We suggest an incubation period of 30-60 minutes at room temperature.
However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user.
Enzymatic predigestion with proteolytic enzymes is recommended for paraffin embedded tissue sections.
This antibody is not recommended for frozen sections or cell smears.
靶标
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功能Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
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组织特异性Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites.
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序列相似性Belongs to the LAMP family.
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翻译后修饰N- and O-glycosylated.
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细胞定位Cell membrane and Endosome membrane. Lysosome membrane.
- Information by UniProt
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数据库链接
- Entrez Gene: 968 Human
- Omim: 153634 Human
- SwissProt: P34810 Human
- Unigene: 647419 Human
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别名
- CD 68 antibody
- CD68 antibody
- CD68 antigen antibody
see all
图片
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [PG-M1], prediluted (ab844)](https://www.abcam.cn/ps/datasheet/images/0/ab844/CD68-Primary-antibodies-ab844-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [PG-M1], prediluted (ab844)ab844 staining CD68 in formalin-fixed, paraffin-embedded Human tonsil tissue by Immunohistochemistry. Staining was detected using DAB.
数据表及文件
文献
This product has been referenced in:
- Tewari A et al. Upregulation of MMP12 and its activity by UVA1 in human skin: potential implications for photoaging. J Invest Dermatol 134:2598-609 (2014). IF . Read more (PubMed: 24714202) »
