Anti-CD68抗体[PG-M1] (ab783)

Reviews (2)Q&A (2)References (7)

概述

  • 产品名称

    Anti-CD68抗体[PG-M1]
    参阅全部 CD68 一抗

  • 描述

    小鼠单克隆抗体[PG-M1] to CD68

  • 宿主

    Mouse

  • 经测试应用

    适用于: IHC-Pmore details
    不适用于: ICC or IHC-Fr

  • 种属反应性

    与反应: Human

  • 免疫原

    Gaucher cells

  • 阳性对照

    • IHC-P: Human tonsil and granulomatous inflammatory tissues.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituents: Tissue culture supernatant, 1% BSA
  • Concentration information loading...

  • 纯度

    Tissue culture supernatant

  • 克隆

    单克隆

  • 克隆编号

    PG-M1

  • 同种型

    IgG3

  • 轻链类型

    kappa

  • 研究领域

应用

Our Abpromise guarantee covers the use of ab783 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/10 - 1/40. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. (use ABC method). We suggest an incubation period of 30-60 minutes at room temperature.
  • 应用说明
    Is unsuitable for ICC or IHC-Fr.

    • 靶标

    • 功能

      Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.

    • 组织特异性

      Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites.

    • 序列相似性

      Belongs to the LAMP family.

    • 翻译后修饰

      N- and O-glycosylated.

    • 细胞定位

      Cell membrane and Endosome membrane. Lysosome membrane.
    • Information by UniProt

    • 数据库链接

      • 别名

        • CD 68 antibody
        • CD68 antibody
        • CD68 antigen antibody
        • CD68 molecule antibody
        • CD68_HUMAN antibody
        • DKFZp686M18236 antibody
        • gp11 antibody
        • Gp110 antibody
        • LAMP4 antibody
        • Macrophage antigen CD68 (microsialin) antibody
        • MACROPHAGE ANTIGEN CD68 antibody
        • Macrosialin antibody
        • SCARD1 antibody
        • Scavenger receptor class D member 1 antibody
        see all

      图片

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [PG-M1] (ab783)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [PG-M1] (ab783)

        ab783 staining CD68 in Human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [PG-M1] (ab783)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [PG-M1] (ab783)This image is courtesy of an anonymous Abreview.
        Paraformaldehyde-fixed, paraffin-embedded human granulomatous inflammatory tissue stained for CD68 using ab783 in immunohistochemical analysis.

        See Abreview

      文献

      This product has been referenced in:

      • Waller R  et al. Iba-1-/CD68+ microglia are a prominent feature of age-associated deep subcortical white matter lesions. PLoS One 14:e0210888 (2019). Read more (PubMed: 30682074) »
      • Pratt D  et al. Programmed Death Ligand 1 Is a Negative Prognostic Marker in Recurrent Isocitrate Dehydrogenase-Wildtype Glioblastoma. Neurosurgery N/A:N/A (2018). IHC-P . Read more (PubMed: 30011045) »
      See all 7 Publications for this product

      发表研究结果有使用 ab783？请让我们知道，以便我们可以引用本数据表中的参考文章。

      客户评价及客户问答

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      1-4 of 4 Abreviews or Q&A

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD68 antibody [PG-M1]

       Excellent
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Human Tissue sections (Meninges)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Tris-EDTA ph9
      Permeabilization
      Yes - 0.1% tween in PBS
      Specification
      Meninges
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
      Fixative
      Formaldehyde
      Read More

      Abcam user community

      Verified customer

      提交于 Jan 02 2020

      Question

      Telefon call:

      Wants to label ab783 or ab844 with AF488,
      What is in the buffer of ab844? Will it interfere with the labeling reaction, what is the concentration?

      What is the concentration of ab783?

      Read More
      Answer

      Thank you for your inquiry.

      I apologise for the delay. I had to wait for an answer from the source of these two products.

      I can now confirm that the concentration of ab783 is 10ug/ml and ab844 is approximately 2-4ug/ml.

      They also contain 0.05% sodium azide which might interfere with the conjugation reaction.

      I hope this information is helpful and wish you a good weekend.

      Read More

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD68 antibody [PG-M1]

       Excellent
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Human Tissue sections (granulomatous inflammatory tissue)
      Specification
      granulomatous inflammatory tissue
      Fixative
      Paraformaldehyde
      Antigen retrieval step
      Enzymatic - Buffer/Enzyme Used: Pepsin
      Permeabilization
      No
      Blocking step
      Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
      Read More

      Abcam user community

      Verified customer

      提交于 Jul 21 2008

      Question

      We are looking for antibodies to easily tell the difference between astocytes, oligodendrocytes and macrophages (including microglia) in mature human brain tissues. Goal is to use them in double immunohistochemistry staining on formalin fixed, paraffin embedded tissues. Is this CD68 antibody proper for this purpose ?

      Read More
      Answer

      Thank you for your enquiry regarding ab783. We have received feedback from the source of the antibody telling us that "this CD68 is specific against macrophages only" unfortunately. I would like to recommend looking at the following page from our website to help you find markers of each cell population: http://abcam2/index.html?pageconfig=resource&rid=295&pid=7 I hope this information will help you, please do not hesitate to contact us again if you need further assistance,

      Read More

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