Anti-CD68抗体[KP1],prediluted (ab845)

概述

  • 产品名称
    Anti-CD68抗体[KP1],prediluted
    参阅全部 CD68 一抗
  • 描述
    小鼠单克隆抗体[KP1] to CD68,prediluted
  • 宿主
    Mouse
  • 特异性
    This antibody is specific to macrophages in a wide variety of human tissues. It reacts with myeloid precursors and peripheral blood granulocytes. It also stains a cell population known as Plasmacytoid T cells.
  • 经测试应用
    适用于: Flow Cyt, IHC-P, IHC-Fr, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Subcellular fraction of human alveolar macrophages.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • 存储溶液
    Preservative: 0.05% Sodium Azide
    Constituents: Inert stabilizer
  • Concentration information loading...
  • 纯化说明
    Purified from TCS
  • 克隆
    单克隆
  • 克隆编号
    KP1
  • 骨髓瘤
    unknown
  • 同种型
    IgG1
  • 轻链类型
    kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab845 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt 1/4.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

IHC-P Use at an assay dependent concentration.
Proteolytic treatment is required prior to immunostaining of formalin fixed paraffin embedded tissue sections. High temperature antigen unmasking may improve the staining.
IHC-Fr Use at an assay dependent concentration. Acetone fixed.
ICC/IF Use at an assay dependent concentration.

靶标

  • 功能
    Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
  • 组织特异性
    Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites.
  • 序列相似性
    Belongs to the LAMP family.
  • 翻译后修饰
    N- and O-glycosylated.
  • 细胞定位
    Cell membrane and Endosome membrane. Lysosome membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD 68 antibody
    • CD68 antibody
    • CD68 antigen antibody
    • CD68 molecule antibody
    • CD68_HUMAN antibody
    • DKFZp686M18236 antibody
    • gp11 antibody
    • Gp110 antibody
    • LAMP4 antibody
    • Macrophage antigen CD68 (microsialin) antibody
    • MACROPHAGE ANTIGEN CD68 antibody
    • Macrosialin antibody
    • SCARD1 antibody
    • Scavenger receptor class D member 1 antibody
    see all

图片

  • Overlay histogram showing THP1 cells stained with ab845 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab845, 1/4 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

文献

This product has been referenced in:
  • Wu SQ  et al. Prognostic roles of tumor associated macrophages in bladder cancer: a system review and meta-analysis. Oncotarget 9:25294-25303 (2018). Read more (PubMed: 29861872) »
  • Zeis T  et al. Molecular pathology of Multiple Sclerosis lesions reveals a heterogeneous expression pattern of genes involved in oligodendrogliogenesis. Exp Neurol 305:76-88 (2018). IHC-Fr ; Human . Read more (PubMed: 29596844) »

See all 9 Publications for this product

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