Anti-CD68抗体[KP1] (ab955)
- 数据表
- 参考文献 (177)
- 实验方案
概述
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产品名称Anti-CD68抗体[KP1]
参阅全部 CD68 一抗 -
描述小鼠单克隆抗体[KP1] to CD68
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宿主Mouse
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经测试应用适用于: ICC/IF, IHC-FoFr, IHC-P, IHC-Frmore details
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种属反应性与反应: Human
预测可用于: Mouse, Rat, Rabbit
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阳性对照
- IHC-P: Human tonsil tissue.
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常规说明
We have received mixed customer feedback regarding suitability of ab955 with mouse and rat tissue and therefore no longer recommend it for mouse and rat. Please note it will also not be covered by our AbPromise guarantee for these species.
Abcam recommended secondaries - Goat Anti-Mouse HRP (ab205719) and Goat Anti-Mouse Alexa Fluor® 488 (ab150113).
See other anti-mouse secondary antibodies that can be used with this antibody.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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存储溶液Preservative: 0.099% Sodium azide
Constituents: 0.9% Proprietary component, Water -
Concentration information loading... -
纯度Protein A/G purified
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克隆单克隆
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克隆编号KP1
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骨髓瘤unknown
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同种型IgG1
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轻链类型kappa
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研究领域
相关产品
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Alternative Products
- Anti-CD68 antibody [FA-11] (Alexa Fluor® 488) (ab201844)
- Anti-CD68 antibody [FA-11] (Alexa Fluor® 647) (ab201845)
- Anti-CD68 antibody [EPR20545] (ab213363)
- Anti-CD68 antibody [KP1] (Alexa Fluor® 488) (ab222914)
- Anti-CD68 antibody [EPR20545] (Alexa Fluor® 647) (ab224029)
- Anti-CD68 antibody [EPR20545] - BSA and Azide free (ab227458)
- Anti-CD68 antibody [FA-11] (ab53444)
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Alternative Versions
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Antibody Diluent
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Compatible Secondaries
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Immunohistochemistry kits
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Isotype control
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Recombinant Protein
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Related Products
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Unmasking reagent
应用
Our Abpromise guarantee covers the use of ab955 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. PubMed: 18804859 | |
| IHC-FoFr | Use at an assay dependent concentration. | |
| IHC-P | 1/100 - 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Recommended heat mediated antigen retrieval in pressure cooker for 2-5 minutes or steaming of tissue sections for 45-60 minutes followed by cooling for 10 minutes and washing in distilled water. In case of enzymatic epitope retrieval - digest with Pepsin enzyme for 30-60 seconds at RT. |
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| IHC-Fr | Use at an assay dependent concentration. |
靶标
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功能Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
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组织特异性Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites.
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序列相似性Belongs to the LAMP family.
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翻译后修饰N- and O-glycosylated.
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细胞定位Cell membrane and Endosome membrane. Lysosome membrane.
- Information by UniProt
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数据库链接
- Entrez Gene: 968 Human
- Entrez Gene: 12514 Mouse
- Entrez Gene: 287435 Rat
- Omim: 153634 Human
- SwissProt: P34810 Human
- SwissProt: P31996 Mouse
- Unigene: 647419 Human
- Unigene: 15819 Mouse
see all -
别名
- CD 68 antibody
- CD68 antibody
- CD68 antigen antibody
see all
图片
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1] (ab955)](https://www.abcam.cn/ps/products/0/ab955/Images/ab955-263251-ab955IHC.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1] (ab955)Image is courtesy of an anonymous AbReviewab955 staining CD68 in human spleen tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Tissue was fixed with formaldehyde and blocked with 10% serum for 10 minutes at 20°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/200) for 30 minutes at 20°C. An undiluted HRP-conjugated rat anti-mouse/rabbit polymer was used as the secondary antibody.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1] (ab955)](https://www.abcam.cn/ps/products/0/ab955/Images/CD68-Primary-antibodies-ab955-7.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1] (ab955)Image courtesy of an anonymous Abreview.ab955 staining CD68 in human liver tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Tissue was fixed with paraformaldehyde. Samples were then blocked with 10% serum for 3 hours at 22°C followed by incubation with the primary antibody at a 1/100 dilution for 16 hours at 4°C. An Alexa-Fluor® 568 conjugated goat anti-mouse polyclonal was used as secondary antibody at a 1/400 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1] (ab955)](https://www.abcam.cn/ps/products/0/ab955/Images/CD68-Primary-antibodies-ab955-3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1] (ab955)This image is courtesy of an anonymous Abreviewab955 staining CD68 in human ulcerated oral (Mucosa/Bone) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Tissue was fixed with paraformaldehyde, permeabilized with 0.1% Triton-X 100 in PBS and blocked with 2.5% serum for 90 minutes at 25°C; antigen retrieval was by heat mediation in citrate buffer (pH 6). Samples were incubated with primary antibody (1/500 in 1% serum in PBS +0.01% Triton-X 100) for 16 hours at 4°C. A commercial IHC kit and DAB was used to visualize the staining.
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![Immunocytochemistry/ Immunofluorescence - Anti-CD68 antibody [KP1] (ab955)](https://www.abcam.cn/ps/products/0/ab955/Images/ab955-263273-955-ICC.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CD68 antibody [KP1] (ab955)Yuan Z et al., PLoS One 9:e94241 (2014)., doi: 10.1371/journal.pone.0094241ab955 staining CD68 in human lung cancer tissues by ICC/IF (Immunocytochemistry/immunofluorescence).
Cells were fixed with formalin and blocked with 2% horse serum for 1 hour at room temperature. Samples were then incubated with primary antibody (1/100) for 1 hour at 21°C. An Alexa Fluor® 488-conjugated Donkey anti-mouse IgG polyclonal (1/500) was used as the secondary antibody. DAPI containing mounting medium was used for nuclear staining (blue) and anti-TREM-1 (1/500) used for TREM-1 staining (red).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1] (ab955)](https://www.abcam.cn/ps/products/0/ab955/Images/ab955-284914-ab955ich.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1] (ab955)Immunohistochemical analysis of tonsil labeling CD68 with ab955.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1] (ab955)](https://www.abcam.cn/ps/products/0/ab955/Images/ab955-252273-955.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1] (ab955)Immunohistochemical analysis of tonsil labeling CD68 with ab955.
文献
This product has been referenced in:
- Mei F et al. Magnesium isoglycyrrhizinate alleviates liver injury in obese rats with acute necrotizing pancreatitis. Pathol Res Pract 215:106-114 (2019). Read more (PubMed: 30396756) »
- Li L et al. The Spleen Promotes the Secretion of CCL2 and Supports an M1 Dominant Phenotype in Hepatic Macrophages During Liver Fibrosis. Cell Physiol Biochem 51:557-574 (2018). Read more (PubMed: 30458454) »
