Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [KP1] to CD68
- Suitable for: WB, ICC, IHC-P
- Reacts with: Human
参阅全部 CD68 一抗
描述小鼠单克隆抗体[KP1] to CD68
经测试应用适用于: WB, ICC, IHC-Pmore details
预测可用于: Mouse, Rat, Rabbit
- IHC-P: human tonsil and liver tissues; ICC: THP-1 cells; WB: Human fetal spleen lysate.
Anti CD68 antibody (ab955) is reccomended for IHC on human samples but is not reccomended for mouse & rat samples.
This product has switched from a hybridoma to recombinant production method on 21st September 2020.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
纯度Protein A purified
Our Abpromise guarantee covers the use of ab955 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 37 kDa.|
|IHC-P||1/3000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
功能Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
组织特异性Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites.
序列相似性Belongs to the LAMP family.
翻译后修饰N- and O-glycosylated.
细胞定位Cell membrane and Endosome membrane. Lysosome membrane.
- Information by UniProt
- CD 68 antibody
- CD68 antibody
- CD68 antigen antibody
Anti-CD68 antibody [KP1] (ab955) at 1/1000 dilution + Human fetal spleen lysate at 20 µg
Peroxidase-Conjugated Goat anti-Mouse IgG(H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size: 37 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Exposure time: 70 seconds
The observed molecular weight is consistent with the literature (PMID:18405323; PMID:11739566; PMID: 16710801).
Immunocytochemistry analysis of THP-1 (human monocytic leukemia monocyte) labelling CD28 with ab955 at 1/50 dilution. Cells were fixed with 100% methanol. Goat Anti-mouse IgG H&L (Alexa Fluor® 488) (ab150113) at 1/1000 was used as the secondary antibody (green). Cells were counterstained with Anti-Tubulin antibody (rabbit mAb), ab179504 - AlexaFluor®594 Goat anti- Rabbit secondary, ab150080 at 1/500 dilution (red). Nuclear DNA was labelled with DAPI (blue).
Confocal image showing cytoplasmic staining in THP-1 cells
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue labelling CD68 with ab955 at 1/3000 dilution. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab93684 a Goat Anti-mouse IgG H&L (HRP) was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Cytoplasmic staining on Kupffer cells of human liver (PMID: 12118106).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue labelling CD68 with ab955 at 1/3000 dilution. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab93684 a Goat Anti-mouse IgG H&L (HRP) was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Cytoplasmic staining on macrophages of human tonsil (PMID: 19543531).
ab955 staining CD68 in human liver tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Tissue was fixed with paraformaldehyde. Samples were then blocked with 10% serum for 3 hours at 22°C followed by incubation with the primary antibody at a 1/100 dilution for 16 hours at 4°C. An Alexa-Fluor® 568 conjugated goat anti-mouse polyclonal was used as secondary antibody at a 1/400 dilution.
ab955 staining CD68 in human ulcerated oral (Mucosa/Bone) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Tissue was fixed with paraformaldehyde, permeabilized with 0.1% Triton-X 100 in PBS and blocked with 2.5% serum for 90 minutes at 25°C; antigen retrieval was by heat mediation in citrate buffer (pH 6). Samples were incubated with primary antibody (1/500 in 1% serum in PBS +0.01% Triton-X 100) for 16 hours at 4°C. A commercial IHC kit and DAB was used to visualize the staining.
ab955 被引用在 313 文献中.
- Li L et al. XIST/miR-376c-5p/OPN axis modulates the influence of proinflammatory M1 macrophages on osteoarthritis chondrocyte apoptosis. J Cell Physiol 235:281-293 (2020). PubMed: 31215024
- Wang J et al. Low immune index correlates with favorable prognosis but with reduced benefit from chemotherapy in gallbladder cancer. Cancer Sci 111:219-228 (2020). PubMed: 31729088
- Liu X et al. Primary Alcohol-Activated Human and Mouse Hepatic Stellate Cells Share Similarities in Gene-Expression Profiles. Hepatol Commun 4:606-626 (2020). PubMed: 32258954
- McCormick A et al. Placental endothelin-converting enzyme-1 is decreased in preeclampsia. Pregnancy Hypertens 20:108-110 (2020). PubMed: 32278308
- Dan H et al. RACK1 promotes cancer progression by increasing the M2/M1 macrophage ratio via the NF-?B pathway in oral squamous cell carcinoma. Mol Oncol 14:795-807 (2020). PubMed: 31997535