Anti-CD68抗体[KP1] (ab955)

Immunohistochemical analysis of tonsil labeling CD68 with ab955.

ab955 staining CD68 in human lung cancer tissues by ICC/IF (Immunocytochemistry/immunofluorescence).

Cells were fixed with formalin and blocked with 2% horse serum for 1 hour at room temperature. Samples were then incubated with primary antibody (1/100) for 1 hour at 21°C. An Alexa Fluor^® 488-conjugated Donkey anti-mouse IgG polyclonal (1/500) was used as the secondary antibody. DAPI containing mounting medium was used for nuclear staining (blue) and anti-TREM-1 (1/500) used for TREM-1 staining (red).

ab955 staining CD68 in human spleen tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

Tissue was fixed with formaldehyde and blocked with 10% serum for 10 minutes at 20°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/200) for 30 minutes at 20°C. An undiluted HRP-conjugated rat anti-mouse/rabbit polymer was used as the secondary antibody.

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ab955 staining CD68 in human liver tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

Tissue was fixed with paraformaldehyde. Samples were then blocked with 10% serum for 3 hours at 22°C followed by incubation with the primary antibody at a 1/100 dilution for 16 hours at 4°C. An Alexa-Fluor^® 568 conjugated goat anti-mouse polyclonal was used as secondary antibody at a 1/400 dilution.

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Immunohistochemical analysis of tonsil labeling CD68 with ab955.

ab955 staining CD68 in human ulcerated oral (Mucosa/Bone) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

Tissue was fixed with paraformaldehyde, permeabilized with 0.1% Triton-X 100 in PBS and blocked with 2.5% serum for 90 minutes at 25°C; antigen retrieval was by heat mediation in citrate buffer (pH 6). Samples were incubated with primary antibody (1/500 in 1% serum in PBS +0.01% Triton-X 100) for 16 hours at 4°C. A commercial IHC kit and DAB was used to visualize the staining.

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