概述

  • 产品名称
    Anti-CD68抗体[KP1]
    参阅全部 CD68 一抗
  • 描述
    小鼠单克隆抗体[KP1] to CD68
  • 宿主
    Mouse
  • 经测试应用
    适用于: ICC/IF, IHC-FoFr, IHC-P, IHC-Frmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit
  • 阳性对照
    • IHC-P: Human tonsil tissue.
  • 常规说明

    We have received mixed customer feedback regarding suitability of ab955 with mouse and rat tissue and therefore no longer recommend it for mouse and rat. Please note it will also not be covered by our AbPromise guarantee for these species.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.099% Sodium azide
    Constituents: 0.9% Proprietary component, Water
  • Concentration information loading...
  • 纯度
    Protein A/G purified
  • 克隆
    单克隆
  • 克隆编号
    KP1
  • 骨髓瘤
    unknown
  • 同种型
    IgG1
  • 轻链类型
    kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab955 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration. PubMed: 18804859
IHC-FoFr Use at an assay dependent concentration.
IHC-P 1/100 - 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Recommended heat mediated antigen retrieval in pressure cooker for 2-5 minutes or steaming of tissue sections for 45-60 minutes followed by cooling for 10 minutes and washing in distilled water.

In case of enzymatic epitope retrieval - digest with Pepsin enzyme for 30-60 seconds at RT.

IHC-Fr Use at an assay dependent concentration.

靶标

  • 功能
    Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
  • 组织特异性
    Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites.
  • 序列相似性
    Belongs to the LAMP family.
  • 翻译后修饰
    N- and O-glycosylated.
  • 细胞定位
    Cell membrane and Endosome membrane. Lysosome membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD 68 antibody
    • CD68 antibody
    • CD68 antigen antibody
    • CD68 molecule antibody
    • CD68_HUMAN antibody
    • DKFZp686M18236 antibody
    • gp11 antibody
    • Gp110 antibody
    • LAMP4 antibody
    • Macrophage antigen CD68 (microsialin) antibody
    • MACROPHAGE ANTIGEN CD68 antibody
    • Macrosialin antibody
    • SCARD1 antibody
    • Scavenger receptor class D member 1 antibody
    see all

图片

  • ab955 staining CD68 in human spleen tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

    Tissue was fixed with formaldehyde and blocked with 10% serum for 10 minutes at 20°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/200) for 30 minutes at 20°C. An undiluted HRP-conjugated rat anti-mouse/rabbit polymer was used as the secondary antibody.

    See Abreview

  • ab955 staining CD68 in human liver tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

    Tissue was fixed with paraformaldehyde. Samples were then blocked with 10% serum for 3 hours at 22°C followed by incubation with the primary antibody at a 1/100 dilution for 16 hours at 4°C. An Alexa-Fluor® 568 conjugated goat anti-mouse polyclonal was used as secondary antibody at a 1/400 dilution.

    See Abreview

  • ab955 staining CD68 in human ulcerated oral (Mucosa/Bone) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

    Tissue was fixed with paraformaldehyde, permeabilized with 0.1% Triton-X 100 in PBS and blocked with 2.5% serum for 90 minutes at 25°C; antigen retrieval was by heat mediation in citrate buffer (pH 6). Samples were incubated with primary antibody (1/500 in 1% serum in PBS +0.01% Triton-X 100) for 16 hours at 4°C. A commercial IHC kit and DAB was used to visualize the staining.

    See Abreview

  • ab955 staining CD68 in human lung cancer tissues by ICC/IF (Immunocytochemistry/immunofluorescence).

    Cells were fixed with formalin and blocked with 2% horse serum for 1 hour at room temperature. Samples were then incubated with primary antibody (1/100) for 1 hour at 21°C. An Alexa Fluor® 488-conjugated Donkey anti-mouse IgG polyclonal (1/500) was used as the secondary antibody. DAPI containing mounting medium was used for nuclear staining (blue) and anti-TREM-1 (1/500) used for TREM-1 staining (red).

  • Immunohistochemical analysis of tonsil labeling CD68 with ab955.

  • Immunohistochemical analysis of tonsil labeling CD68 with ab955.

文献

This product has been referenced in:
  • You S  et al. An Aza resveratrol-chalcone derivative 6b protects mice against diabetic cardiomyopathy by alleviating inflammation and oxidative stress. J Cell Mol Med 22:1931-1943 (2018). Read more (PubMed: 29327811) »
  • Kim SJ  et al. Anti-inflammatory effect of Tauroursodeoxycholic acid in RAW 264.7 macrophages, Bone marrow-derived macrophages, BV2 microglial cells, and spinal cord injury. Sci Rep 8:3176 (2018). Read more (PubMed: 29453346) »
See all 169 Publications for this product

客户评价及客户问答

1-10 of 50 Abreviews or Q&A

Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Heart)
Permeabilization
No
Specification
Heart
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 28°C
Fixative
Acetone

Abcam user community

Verified customer

提交于 Dec 18 2017

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (stomach)
Permeabilization
Yes - 0.05% TX-100
Specification
stomach
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

提交于 Aug 28 2017

Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (liver)
Permeabilization
Yes - 0.05% Triton X-100
Specification
liver
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

提交于 Jun 27 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citrate pH6
Permeabilization
No
Specification
lung
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

提交于 Apr 17 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (spleen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH6
Permeabilization
No
Specification
spleen
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
10% normal buffered formalin

Abcam user community

Verified customer

提交于 Feb 28 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (heart)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate buffer pH6
Permeabilization
No
Specification
heart
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
10% Normal buffered formalin

Abcam user community

Verified customer

提交于 Feb 07 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (spleen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citrate pH6
Permeabilization
No
Specification
spleen
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
10% Normal buffered formalin

Abcam user community

Verified customer

提交于 Jan 31 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Sheep Tissue sections (Mitral valve, vascular interstitial cells)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Antigen Decloaker
Permeabilization
Yes - 0.2% Triton X-100
Specification
Mitral valve, vascular interstitial cells
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Fixative
Formaldehyde

Abcam user community

Verified customer

提交于 Aug 17 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (muscle)
Permeabilization
No
Specification
muscle
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Formaldehyde

Abcam user community

Verified customer

提交于 Mar 14 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Mammary Gland Tissue)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate 10mM pH=6.0
Permeabilization
Yes - PBS with 3% Triton
Specification
Mammary Gland Tissue
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Formaldehyde

Abcam user community

Verified customer

提交于 Aug 04 2015

1-10 of 50 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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