Anti-CD68抗体[C68/684] (ab201340)
Key features and details
- Mouse monoclonal [C68/684] to CD68
- Suitable for: WB, Flow Cyt, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-CD68抗体[C68/684]
参阅全部 CD68 一抗 -
描述
小鼠单克隆抗体[C68/684] to CD68 -
宿主
Mouse -
特异性
ab201340 recognizes a glycoprotein of 110kDa, which is identified as CD68. -
经测试应用
适用于: WB, Flow Cyt, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Monkey -
免疫原
Recombinant full length protein corresponding to Human CD68. (Subcellular fraction of Human alveolar macrophages).
Database link: P34810 -
阳性对照
- IHC-P: Human tonsil and histiocytoma tissues; ICC/IF: U-87 MG cells; Flow Cyt: U-87 MG cells; WB: Human spleen tissue lysate.
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常规说明
ab201340 stains macrophages in a wide variety of Human tissues, including Kupffer cells and macrophages in the red pulp of the spleen, in lamina propria of the gut, in lung alveoli, and in bone marrow. It reacts with myeloid precursors and peripheral blood granulocytes. It also reacts with plasmacytoid T cells, which are supposed to be of monocyte/macrophage origin. It shows strong granular cytoplasmic staining of chronic and acute myeloid leukemia and also reacts with rare cases of true histiocytic neoplasia. Lymphomas are negative or show few granules.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA -
Concentration information loading...
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纯度
Protein A/G purified -
纯化说明
ab201340 is purified from Bioreactor Concentrate by Protein A/G column chromatography. -
克隆
单克隆 -
克隆编号
C68/684 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab201340于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 37 kDa).
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Flow Cyt | (1) |
Use 1-2µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
IHC-P | (2) |
Use a concentration of 1 - 2 µg/ml.
Perform heat mediated antigen retrieval with 10 mM Tris with 1 mM EDTA buffer pH 9.0 for 45 minutes at 95°C followed by cooling at RT for 20 minutes. Incubate with primary for 30 minutes at RT. |
ICC/IF |
Use a concentration of 1 - 2 µg/ml.
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说明 |
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WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 37 kDa). |
Flow Cyt
Use 1-2µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with 10 mM Tris with 1 mM EDTA buffer pH 9.0 for 45 minutes at 95°C followed by cooling at RT for 20 minutes. Incubate with primary for 30 minutes at RT. |
ICC/IF
Use a concentration of 1 - 2 µg/ml. |
靶标
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功能
Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells. -
组织特异性
Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites. -
序列相似性
Belongs to the LAMP family. -
翻译后修饰
N- and O-glycosylated. -
细胞定位
Cell membrane and Endosome membrane. Lysosome membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 968 Human
- Omim: 153634 Human
- SwissProt: P34810 Human
- Unigene: 647419 Human
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别名
- CD 68 antibody
- CD68 antibody
- CD68 antigen antibody
see all
图片
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue labeling CD68 with ab201340 at 1 µg/ml.
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Flow Cytometry analysis of U-87 MG (human glioblastoma-astrocytoma epithelial cell line) cells labeling CD68 with ab201340. Goat Anti-Mouse IgG was used as the secondary antibody (blue); Isotype Control (red).
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Immunocytochemistry/immunofluorescence analysis of U-87 MG (human glioblastoma-astrocytoma epithelial cell line) cells labelling CD68 with ab201340. Goat Anti-Mouse IgG was used as the secondary antibody (green). Nuclear DNA stained red.
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Anti-CD68 antibody [C68/684] (ab201340) + Human spleen tissue lysate
Predicted band size: 37 kDa
Additional bands at: 110 kDa (possible glycosylated form)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (81)
ab201340 被引用在 81 文献中.
- Tsai MT et al. Plasma Galectin-9 Is a Useful Biomarker for Predicting Renal Function in Patients Undergoing Native Kidney Biopsy. Arch Pathol Lab Med 147:167-176 (2023). PubMed: 35687787
- Wang Y et al. Peritoneal M2 macrophage-derived extracellular vesicles as natural multitarget nanotherapeutics to attenuate cytokine storms after severe infections. J Control Release 349:118-132 (2022). PubMed: 35792186
- Shi C et al. SIRT3 inhibitor 3-TYP exacerbates thioacetamide-induced hepatic injury in mice. Front Physiol 13:915193 (2022). PubMed: 35923224
- Deng Y et al. Hyperhomocysteinemia Promotes Cardiac Hypertrophy in Hypertension. Oxid Med Cell Longev 2022:1486157 (2022). PubMed: 36046692
- Yuan X et al. RGMa promotes dedifferentiation of vascular smooth muscle cells into a macrophage-like phenotype in vivo and in vitro. J Lipid Res 63:100276 (2022). PubMed: 36089003