Anti-CD63抗体[NKI/C3] - BSA and Azide free (ab213090)
Key features and details
- Mouse monoclonal [NKI/C3] to CD63 - BSA and Azide free
- Suitable for: WB, Flow Cyt, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG1
Related conjugates and formulations
概述
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产品名称
Anti-CD63抗体[NKI/C3] - BSA and Azide free
参阅全部 CD63 一抗 -
描述
小鼠单克隆抗体[NKI/C3] to CD63 - BSA and Azide free -
宿主
Mouse -
经测试应用
适用于: WB, Flow Cyt, IHC-Pmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Tissue, cells or virus. Smooth plasma membrane fraction of MeWo cells
Database link: P08962 -
阳性对照
- Flow Cytometry: MCF7 cells. IHC-P: Mouse spleen tissue. Human melanoma tissue. WB: HepG2 cell lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Constituent: PBS -
无载体
是 -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
NKI/C3 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab213090于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 0.5 - 1 µg/ml.
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Flow Cyt |
Use 0.5-1µg for 106 cells.
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IHC-P |
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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WB
Use a concentration of 0.5 - 1 µg/ml. |
Flow Cyt
Use 0.5-1µg for 106 cells. |
IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
This antigen is associated with early stages of melanoma tumor progression. May play a role in growth regulation. -
组织特异性
Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages. -
序列相似性
Belongs to the tetraspanin (TM4SF) family. -
细胞定位
Cell membrane. Lysosome membrane. Late endosome membrane. Also found in Weibel-Palade bodies of endothelial cells. Located in platelet dense granules. - Information by UniProt
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数据库链接
- Entrez Gene: 967 Human
- Entrez Gene: 12512 Mouse
- Omim: 155740 Human
- SwissProt: P08962 Human
- SwissProt: P41731 Mouse
- Unigene: 445570 Human
- Unigene: 371552 Mouse
- Unigene: 416079 Mouse
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别名
- Lysosomal associated membrane protein 3 antibody
- CD 63 antibody
- CD63 antibody
see all
图片
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Anti-CD63 antibody [NKI/C3] - BSA and Azide free (ab213090) at 1 µg/ml + HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate
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Immunohistochemical analysis of paraffin embedded, formalin fixed mouse spleen tissue labeling CD63 with ab213090 at 1 μg/mL dilution. DAB detection.
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Immunohistochemical analysis of paraffin embedded, formalin fixed human melanoma tissue labeling CD63 with ab213090 at 1 μg/mL dilution. EAC chromogen labeling.
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Flow cytometry analysis of MCF7 (Human breast adenocarcinoma cell line) cells labeling CD63 using PE conjugated ab213090 (Red). Isotype control in Green; cells alone in black.
实验方案
数据表及文件
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Datasheet download
文献 (19)
ab213090 被引用在 19 文献中.
- Duan J et al. Trophoblast Stem-Cell-Derived Exosomes Alleviate Cardiotoxicity of Doxorubicin via Improving Mfn2-Mediated Mitochondrial Fusion. Cardiovasc Toxicol 23:23-31 (2023). PubMed: 36609664
- Chen Y et al. RA Fibroblast-Like Synoviocytes Derived Extracellular Vesicles Promote Angiogenesis by miRNA-1972 Targeting p53/mTOR Signaling in Vascular Endotheliocyte. Front Immunol 13:793855 (2022). PubMed: 35350778
- Zhang Z et al. Macrophage‑derived exosomal miRNA‑155 promotes tubular injury in ischemia‑induced acute kidney injury. Int J Mol Med 50:N/A (2022). PubMed: 35795997
- Wan S et al. Intestine epithelial cell-derived extracellular vesicles alleviate inflammation induced by Clostridioides difficile TcdB through the activity of TGF-β1. Mol Cell Toxicol N/A:1-11 (2022). PubMed: 35967466
- Bao Z et al. Exosomal miR-155-5p derived from glioma stem-like cells promotes mesenchymal transition via targeting ACOT12. Cell Death Dis 13:725 (2022). PubMed: 35986010