重组Anti-CD62P抗体[EPR22850-190] (ab255822)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22850-190] to CD62P
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-CD62P抗体[EPR22850-190]
参阅全部 CD62P 一抗 -
描述
兔单克隆抗体[EPR22850-190] to CD62P -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt,ICC/IF or IP -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HUVEC, Human serum, Human plasma, Human lung and Mouse platelet lysates. IHC-P: Human stomach, Human spleen and Mouse spleen tissues.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR22850-190 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab255822于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/1000.
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|
IHC-P |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. |
说明 |
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WB
1/1000. |
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. |
靶标
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功能
Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1. -
组织特异性
Stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface. -
疾病相关
Defects in SELP may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. -
序列相似性
Belongs to the selectin/LECAM family.
Contains 1 C-type lectin domain.
Contains 1 EGF-like domain.
Contains 9 Sushi (CCP/SCR) domains. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 6403 Human
- Entrez Gene: 20344 Mouse
- Omim: 173610 Human
- SwissProt: P16109 Human
- SwissProt: Q01102 Mouse
- Unigene: 73800 Human
- Unigene: 3337 Mouse
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别名
- Antigen CD62 antibody
- CD62 antibody
- CD62 antigen-like family member P antibody
see all
图片
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All lanes : Anti-CD62P antibody [EPR22850-190] (ab255822) at 1/1000 dilution
Lane 1 : Human serum
Lane 2 : Human plasma
Lane 3 : Human lung lysate
Lane 4 : Mouse platelet
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/2000 dilution (Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG)
Observed band size: 140 kDa why is the actual band size different from the predicted?Blocking and Dilution Buffer and concentration: 5% NFDM/TBST.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 24945938).
Exposure time: Lane 1:15 seconds; Lanes 2-4:8 seconds.
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All lanes : Anti-CD62P antibody [EPR22850-190] (ab255822) at 1/1000 dilution
Lane 1 : HUVEC (human umbilical vein endothelial cell) whole cell lysate
Lane 2 : HEK-293 (human embryonic kidney epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution
Observed band size: 140,90 kDa why is the actual band size different from the predicted?Blocking and Dilution Buffer and concentration: 5% NFDM/TBST.
140KDa and 90KDa respectively represent glycosylated form and non-glycosylated form. The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 24945938). Negative control: HEK-293 (PMID: 7693674).
Exposure time: 26 seconds
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Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling CD62P with ab255822 at 1/100 dilution (5.89 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in megakaryocytes and platelets of mouse spleen (PMID: 2472431). The section was incubated with ab255822 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND&~153;,RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
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Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling CD62P with ab255822 at 1/500 dilution (1.178 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in platelets of human spleen (PMID: 2472431). The section was incubated with ab255822 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND®RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
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Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling CD62P with ab255822 at 1/500 dilution (1.178 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in blood vessels of human stomach (PMID: 2472431). The section was incubated with ab255822 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND®RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (5)
ab255822 被引用在 5 文献中.
- Musiu C et al. Fatal cytokine release syndrome by an aberrant FLIP/STAT3 axis. Cell Death Differ 29:420-438 (2022). PubMed: 34518653
- Chen X et al. The phosphatase PTEN links platelets with immune regulatory functions of mouse T follicular helper cells. Nat Commun 13:2762 (2022). PubMed: 35589797
- Wang C et al. Targeted delivery of fat extract by platelet membrane-cloaked nanocarriers for the treatment of ischemic stroke. J Nanobiotechnology 20:249 (2022). PubMed: 35642036
- Luo X et al. Regulating Acidosis and Relieving Hypoxia by Platelet Membrane-Coated Nanoparticle for Enhancing Tumor Chemotherapy. Front Bioeng Biotechnol 10:885105 (2022). PubMed: 35646869
- Zheng C et al. Characterization of Novel P-Selectin Targeted Complement Inhibitors in Murine Models of Hindlimb Injury and Transplantation. Front Immunol 12:785229 (2021). PubMed: 34899752