Anti-CD62P抗体[AK-6] (ab6632)

Reviews (5)Q&A (5)References (15)
Flow Cytometry - Anti-CD62P antibody [AK-6] (ab6632)

    Key features and details

    • Mouse monoclonal [AK-6] to CD62P
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Isotype: IgG1

    Conjugates logo Related conjugates and formulations

    FITC

    概述

    • 产品名称

      Anti-CD62P抗体[AK-6]
      参阅全部 CD62P 一抗

    • 描述

      小鼠单克隆抗体[AK-6] to CD62P

    • 宿主

      Mouse

    • 特异性

      MCA796 recognises the CD62P glycoprotein, a 140kD molecule expressed by activated platelets and endothelial cells.

    • 经测试应用

      适用于: Flow Cytmore details

    • 种属反应性

      与反应: Human

    • 阳性对照

      • IHC-P: Human renal carcinoma tissue. IHC-Fr: Rhesus monkey myometrium tissue. Flow: Thrombin activated human platelets.

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab6632于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    Flow Cyt
    1/50.

    ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
    说明
    Flow Cyt
    1/50.

    ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

    靶标

    • 功能

      Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1.

    • 组织特异性

      Stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.

    • 疾病相关

      Defects in SELP may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.

    • 序列相似性

      Belongs to the selectin/LECAM family.
      Contains 1 C-type lectin domain.
      Contains 1 EGF-like domain.
      Contains 9 Sushi (CCP/SCR) domains.

    • 细胞定位

      Membrane.
    • Information by UniProt

    • 数据库链接

      • 别名

        • Antigen CD62 antibody
        • CD62 antibody
        • CD62 antigen-like family member P antibody
        • CD62P antibody
        • GMP 140 antibody
        • GMP-140 antibody
        • GMP140 antibody
        • GMRP antibody
        • Granule membrane protein 140 antibody
        • Granulocyte membrane protein antibody
        • GRMP antibody
        • LECAM 3 antibody
        • LECAM3 antibody
        • Leukocyte endothelial cell adhesion molecule 3 antibody
        • Leukocyte-endothelial cell adhesion molecule 3 antibody
        • LYAM3_HUMAN antibody
        • P Selectin antibody
        • P-selectin antibody
        • PADGEM antibody
        • Platelet activation dependent granule-external membrane protein antibody
        • Platelet alpha granule membrane protein antibody
        • PSEL antibody
        • Selectin P (granule membrane protein 140kDa, antigen CD62) antibody
        • SELP antibody
        see all

      图片

      • Flow Cytometry - Anti-CD62P antibody [AK-6] (ab6632)
        Flow Cytometry - Anti-CD62P antibody [AK-6] (ab6632)

        ab6632 staining CD62P in thrombin activated human platelets by Flow Cytometry analysis.

      文献 (15)

      发表研究结果有使用 ab6632？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab6632 被引用在 15 文献中.

      • Sun C  et al. ADAM17-regulated CX3CL1 expression produced by bone marrow endothelial cells promotes spinal metastasis from hepatocellular carcinoma. Int J Oncol 57:249-263 (2020). PubMed: 32319605
      • Zhou P  et al. Interactions between neutrophil extracellular traps and activated platelets enhance procoagulant activity in acute stroke patients with ICA occlusion. EBioMedicine 53:102671 (2020). PubMed: 32114386
      • Zhang K  et al. Activation of brain endothelium by Escherichia coli K1 virulence factor cglD promotes polymorphonuclear leukocyte transendothelial migration. Med Microbiol Immunol 208:59-68 (2019). PubMed: 30171337
      • Bertrand L  et al. Targeting the HIV-infected brain to improve ischemic stroke outcome. Nat Commun 10:2009 (2019). PubMed: 31043599
      • Barranco R  et al. Immunohistochemical expression of P-selectin, SP-A, HSP70, aquaporin 5, and fibronectin in saltwater drowning and freshwater drowning. Int J Legal Med 133:1461-1467 (2019). PubMed: 31222534
      View all Publications for this product

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      1-10 of 10 Abreviews or Q&A

      Immunohistochemistry (Frozen sections) abreview for Anti-CD62P antibody [AK-6]

       Excellent
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Human Tissue sections (Human brain)
      Permeabilization
      No
      Specification
      Human brain
      Blocking step
      Antibody Diluent with Background Reducing (Dako/Agilent) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
      Fixative
      Formaldehyde
      Read More

      Abcam user community

      Verified customer

      提交于 Nov 07 2018

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD62P antibody [AK-6]

       Excellent
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Human Tissue sections (Human brain)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: pH9
      Permeabilization
      No
      Specification
      Human brain
      Blocking step
      (agent) for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
      Fixative
      Formaldehyde
      Read More

      Abcam user community

      Verified customer

      提交于 Oct 25 2018

      Immunohistochemistry (Frozen sections) abreview for Anti-CD62P antibody [AK-6]

       Good
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Rhesus monkey Tissue sections (myometrium)
      Permeabilization
      No
      Specification
      myometrium
      Blocking step
      Serum as blocking agent for 20 minute(s) · Concentration: 2% · Temperature: 25°C
      Fixative
      Formaldehyde
      Read More

      Abcam user community

      Verified customer

      提交于 Jan 22 2018

      Immunohistochemistry (Frozen sections) abreview for Anti-CD62P antibody [AK-6]

       Excellent
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Human Tissue sections (nasal mucosa)
      Specification
      nasal mucosa
      Fixative
      Acetone
      Permeabilization
      No
      Blocking step
      H2O2 as blocking agent for 15 minute(s) · Concentration: 3% · Temperature: 21°C
      Read More

      Mr. Michael Könnecke

      Verified customer

      提交于 Jan 07 2013

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD62P antibody [AK-6]

       Good
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Human Tissue sections (Kidney)
      Specification
      Kidney
      Fixative
      Formaldehyde
      Antigen retrieval step
      Heat mediated
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
      Read More

      Abcam user community

      Verified customer

      提交于 Mar 03 2006

      Question

      Inquiry: Since I attend to measure the expression of P-selectin in IL-4 stimulated human umbilical vein endothelial cells by ELISA, I would like to know if the P-Selectin Antibody (AK6 clone, product code 6632) is recommended for this use.

      Read More
      Answer

      Thank you for contacting us.

      ab6632 has yet not been tested in ELISA application; this however doesn't mean this product is not suitable, the product might be suitable however we do not have data to confirm it.

      On the other hand product ab78440 is tested in ELISA and is fully guaranteed;

      https://www.abcam.com/CD62P-antibody-P8G6-ab78440.html

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Use our products? Submit an Abreview. Earn rewards!
      https://www.abcam.com/abreviews

      Read More

      Question

      No signal in western blots of human platelet lysates compared to previous lot.

      Read More
      Answer

      Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

      I have issued a free of charge replacement.

      Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

      I wish you the best of luck with your research.

      Read More

      Question

      Where on the protein does this antibody bind?

      Read More
      Answer

      Thank you for your enquiry regarding ab6632. Unfortunately this antibody has not been epitope mapped so I cannot tell you exactly which amino acids it recognises. However this clone, AK-6, was studied as part of the Adhesion Structures section of Leucocyte Typing V, Volume 2. In this report the epitope for this clone is localised to the SCR domain of CD62P. I hope this information helps. Please do not hesitate to contact us if you need any more advice or information.

      Read More

      Question

      I have been using ab6632(Mouse monoclonal to P-Selectin) for microscopy and had good results as I mentioned in my last email. Your support told me I could use thia Ab for WB in either non-reducing or reducing conditions. I have tried non reducing conditions, using the Ab at 1:1000 and 1:250 with both secondary to HRP and AP with no results. I am currently doing a reducing western. I would like to know what specific part of the P-Selectin antigen does this Ab bind to? Secondly, does this cross react with E-Selectin?

      Read More
      Answer

      Thank you for your enquiry. This antibody is specific for CD62P. To our knowledge it has not been tested for cross-reactivity with E-selectin (CD62E). As discussed in the reference given below, this antibody was raised against a human platelet glycoprotein which was confirmed to be platelet GMP-140 (or CD62P). The exact epitope recognised by this antibody has yet to be determined. Skinner, M.P. et al. (1989). Characterisation of human platelet GMP-140 as a heparin-binding protein. Biochem Biophys Res Commun. 164(3):1373-1376. We would usually recommend a concentration of 1-10ug/ml for the use of antibodies in Western blotting. Based on this estimate you may need to increase the concentration of antibody used further (i.e. to 1:100). We hope that this information is of assistance.

      Read More

      Question

      Will CD62P work on other species? On formalin fixed tissue?

      Read More
      Answer

      This antibody has been shown to cross-react with human and Rhesus monkey. Other species have not been tested. This antibody is suitable for use on formalin fixed tissue.

      Read More

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