Anti-CD62E抗体[UZ6] (ab2497)

Reviews (1)Q&A (3)References (3)
Immunocytochemistry/ Immunofluorescence - Anti-CD62E antibody [UZ6] (ab2497)

    Key features and details

    • Rat monoclonal [UZ6] to CD62E
    • Suitable for: ICC/IF
    • Reacts with: Mouse
    • Isotype: IgG2b

    概述

    • 产品名称

      Anti-CD62E抗体[UZ6]
      参阅全部 CD62E 一抗

    • 描述

      大鼠单克隆抗体[UZ6] to CD62E

    • 宿主

      Rat

    • 经测试应用

      适用于: ICC/IFmore details

    • 种属反应性

      与反应: Mouse

    • 阳性对照

      • Tonsil

    • 常规说明

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    性能

    应用

    Our Abpromise guarantee covers the use of ab2497 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    ICC/IF Use at an assay dependent concentration.

    靶标

    • 功能

      Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis.

    • 序列相似性

      Belongs to the selectin/LECAM family.
      Contains 1 C-type lectin domain.
      Contains 1 EGF-like domain.
      Contains 6 Sushi (CCP/SCR) domains.

    • 细胞定位

      Membrane.
    • Information by UniProt

    • 数据库链接

      • 别名

        • CD26E antibody
        • CD62 antigen-like family member E antibody
        • CD62E antibody
        • E selectin antibody
        • E-selectin antibody
        • ELAM antibody
        • ELAM-1 antibody
        • ELAM1 antibody
        • Endothelial adhesion molecule 1 antibody
        • Endothelial leukocyte adhesion molecule 1 antibody
        • ESEL antibody
        • LECAM2 antibody
        • Leukocyte-endothelial cell adhesion molecule 2 antibody
        • LYAM2_HUMAN antibody
        • Sele antibody
        • Selectin E antibody
        see all

      图片

      • Immunocytochemistry/ Immunofluorescence - Anti-CD62E antibody [UZ6] (ab2497)
        Immunocytochemistry/ Immunofluorescence - Anti-CD62E antibody [UZ6] (ab2497)

        CHO-cells transfected with E-Selectin stained wtih ab2497 at 1:500. 

      文献 (3)

      发表研究结果有使用 ab2497？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab2497 被引用在 3 文献中.

      • Meng ZD  et al. Effects of RNA interference-mediated E-selectin gene silencing on cell adhesion molecule expression and cell-cell adhesion in vascular endothelial cells in mice with immunologic contact urticaria. J Cell Biochem 120:115-125 (2019). PubMed: 30206961
      • Sapieha P  et al. 5-Lipoxygenase metabolite 4-HDHA is a mediator of the antiangiogenic effect of ?-3 polyunsaturated fatty acids. Sci Transl Med 3:69ra12 (2011). PubMed: 21307302
      • Hammel M  et al. Species-specific and conserved epitopes on mouse and human E-selectin important for leukocyte adhesion. Exp Cell Res 269:266-74 (2001). PubMed: 11570819

      客户评价及客户问答

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      1-4 of 4 Abreviews or Q&A

      Question

      Thank you so much for your email. I would like to ask you a question.

      I have to target 3 proteins in my Mouse Brain Enothelial cell line (bEnd.3), which are E-selectin (CD62E), VCAM and ICAM.

      I have chosen ab2497 for E-selectin , ab61993 for VCAM and ab119871 for ICAM, I would like to know whether these antibodies are going to target the whole length of my molecules, or just bind to specific site of the protein?

      Because I am not sure after my treatment, any of these proteins will be externalised or they will stay in the cytoplasm.

      Read More
      Answer

      Thanks you for your email.
      In my previous email there is a correction about the expiry date. The expiry date should be read as 25-08-2012.
      I have asked lab to release the immunogen information of these antibodies; I will be able to send the answer to your question soon.
      Many thanks for having patience!

      Read More

      Question

      Hi Technical Support, how are you today?

      Could you please advise me on the difference between the corresponding pairs below:

      ab 22631 Anti-CD62E antibody

      &

      ab2497 Anti-CD62E antibody

      and

      ab65472 Quick cell Proliferation assay kit

      &

      ab65474 Quick cell Proliferation assay kit II

      thanks very much for your help!

      In the meantime, have a great day! J

      Kind regards,

      Read More
      Answer

      Thank you very much for your email!

      I hope you are well as well! Here we are fine - we have one of our first days of spring :-)

      In regards to your questions:

      The CD62E antibodies ab22631 et ab2497 are two different clones. Indeed, both are monoclonal rat antibodies, and I would think from the same fusion/productions, however they are two distinct antibodies - once clone UY7 and once clone UZ6. as therefore the epitope is most likely also different, they need therefore to be tested separately for all applications and species reactivity. Please let me know if you would need any additional information.

      For the quick cell proliferation kits, the difference is that the ab65474 contains the stop solution as well, and the ab65472 not.

      I hope this informationis helpful. Please do not hesitate to contact us again should you have any additional questions.

      I wish you a great day as well :-)

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      Immunohistochemistry (Frozen sections) abreview for Anti-CD62E antibody [UZ6]

       Good
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Mouse Tissue sections (Brain)
      Specification
      Brain
      Fixative
      Paraformaldehyde
      Permeabilization
      No
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
      Read More

      Dr. Thomas Weissensteiner

      Verified customer

      提交于 Oct 22 2009

      Question

      1) Crossreactivities? 2) Is it function blocking or not?

      Read More
      Answer

      1) Crossreactivities: this antibody reacts with mouse E-selectin but not with human E-selectin. Other species not tested. 2)Blocking function: Ab2497 (20 microgram/ml) inhibited up to 70% of mouse PMN and up to 95% of human peripheral blood mononuclear cells adhesion to LPS-activated mlEND1 cells. For experimental conditions and figure see Hammel et al., Exp Cell Res 269: 266 (2001).

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