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重组RabMAb

重组Anti-CD5抗体[EP2952] (ab75877)

Submit a review Q&A (1)References (2)
Immunoprecipitation - Anti-CD5 antibody [EP2952] (ab75877)
  • Western blot - Anti-CD5 antibody [EP2952] (ab75877)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [EP2952] (ab75877)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [EP2952] (ab75877)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [EP2952] (ab75877)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [EP2952] (ab75877)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [EP2952] (ab75877)
  • OI-RD Scanning - Anti-CD5 antibody [EP2952] (ab75877)
  • Anti-CD5 antibody [EP2952] (ab75877)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP2952] to CD5
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-CD5抗体[EP2952]
    参阅全部 CD5 一抗

  • 描述

    兔单克隆抗体[EP2952] to CD5

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, IP, IHC-Pmore details
    不适用于: Flow Cyt

  • 种属反应性

    与反应: Human

  • 免疫原

    Synthetic peptide within Human CD5 aa 50-150. The exact sequence is proprietary.

  • 阳性对照

    • WB: Human lymph node lysate IHC-P: Human tonsil tissue. IP: Human lymph node lysate

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab75877于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/10000 - 1/50000. Predicted molecular weight: 55 kDa.
IP
1/50.
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
说明
WB
1/10000 - 1/50000. Predicted molecular weight: 55 kDa.
IP
1/50.
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
应用说明
Is unsuitable for Flow Cyt.

靶标

  • 功能

    May act as a receptor in regulating T-cell proliferation. CD5 interacts with CD72/LYB-2.

  • 序列相似性

    Contains 3 SRCR domains.

  • 细胞定位

    Membrane.
  • Information by UniProt

  • 数据库链接

    • 别名

      • CD 5 antibody
      • CD5 antibody
      • CD5 antigen (p56 62) antibody
      • CD5 antigen antibody
      • CD5 molecule antibody
      • CD5_HUMAN antibody
      • LEU1 antibody
      • LyA antibody
      • Lymphocyte antigen T1/Leu-1 antibody
      • OTTHUMP00000236973 antibody
      • T-cell surface glycoprotein CD5 antibody
      • T1 antibody
      see all

    图片

    • Immunoprecipitation - Anti-CD5 antibody [EP2952] (ab75877)
      Immunoprecipitation - Anti-CD5 antibody [EP2952] (ab75877)

      CD5 was immunoprecipitated from 0.35 mg Human lymph node lysate 10 µg with 75877 at 1/20 dilution (0.6µg) . VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: Human lymph node lysate 10 µg

      Lane 2: ab75877 IP in Human lymph node lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab75877 in Human lymph node lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

       

    • Western blot - Anti-CD5 antibody [EP2952] (ab75877)
      Western blot - Anti-CD5 antibody [EP2952] (ab75877)
      Anti-CD5 antibody [EP2952] (ab75877) at 1/1000 dilution (Purified) + Human lymph node lysate at 15 µg

      Secondary
      Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 55 kDa
      Observed band size: 67 kDa why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [EP2952] (ab75877)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [EP2952] (ab75877)

      ab75877 showing positive staining in Normal colon lymphocytes tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [EP2952] (ab75877)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [EP2952] (ab75877)

      ab75877 showing negative staining in Normal brain tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [EP2952] (ab75877)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [EP2952] (ab75877)

      ab75877 showing negative staining in Normal kidney tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [EP2952] (ab75877)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [EP2952] (ab75877)

      ab75877 showing negative staining in Skeletal muscle tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [EP2952] (ab75877)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [EP2952] (ab75877)

      ab75877 showing positive staining in Normal spleen tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • OI-RD Scanning - Anti-CD5 antibody [EP2952] (ab75877)
      OI-RD Scanning - Anti-CD5 antibody [EP2952] (ab75877)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-CD5 antibody [EP2952] (ab75877)
      Anti-CD5 antibody [EP2952] (ab75877)

    文献 (2)

    发表研究结果有使用 ab75877？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab75877 被引用在 2 文献中.

    • Xiao LI  et al. Multiple myeloma-associated skin light chain amyloidosis: A case of misdiagnosis. Oncol Lett 11:3617-3620 (2016). IHC ; Human . PubMed: 27284363
    • Lu TX  et al. Epstein-Barr virus positive diffuse large B-cell lymphoma predict poor outcome, regardless of the age. Sci Rep 5:12168 (2015). IHC ; Human . PubMed: 26202875

    客户评价及客户问答

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    Question

    I contact you as I would need to have the concentration of one of your antibody. This is apparently a tissue culture supernatant but I would still like to know if you could give me a range of concentration for this antibody. The reference is Ab75877.

    Read More
    Answer

    According to the datasheet, this antibody is indeed a non-purified cell culture supernatantproduct. That means, along with whole serum and Ascitesproducts, the antibody concentration cannot be determined as there are too many other proteins in this solution as well.


    However, we have a table under our Technical FAQs (Question 8, What concentration of primary antibody should I use?) which explains how much specific antibody can be expected in the non-purified products. In this case it is 1 - 3 mg/ml.

    Read More

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