重组Anti-CD46抗体[EPR4014] - Low endotoxin,Azide free (ab229446)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4014] to CD46 - Low endotoxin, Azide free
- Suitable for: IHC-P, WB
- Knockout validated
- Reacts with: Human
概述
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产品名称
Anti-CD46抗体[EPR4014] - Low endotoxin,Azide free
参阅全部 CD46 一抗 -
描述
兔单克隆抗体[EPR4014] to CD46 - Low endotoxin,Azide free -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details
不适用于: Flow Cyt or IP -
种属反应性
与反应: Human
预测可用于: Rabbit -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Wild-type HAP1 cell lysate. MOLT-4, Jurkat, HeLa and K562 cell lysates. IHC-P: Human thyroid gland carcinoma, colonic adenocarcinoma, breast carcinoma, kidney, breast, uterus, placenta and tonsil tissue.
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常规说明
ab229446 is the carrier-free version of ab108307.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.20
Constituent: PBS -
无载体
是 -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR4014 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab229446于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 44 kDa.
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说明 |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 44 kDa. |
靶标
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功能
Acts as a cofactor for complement factor I, a serine protease which protects autologous cells against complement-mediated injury by cleaving C3b and C4b deposited on host tissue. May be involved in the fusion of the spermatozoa with the oocyte during fertilization. Also acts as a costimulatory factor for T-cells which induces the differentiation of CD4+ into T-regulatory 1 cells. T-regulatory 1 cells suppress immune responses by secreting interleukin-10, and therefore are thought to prevent autoimmunity. A number of viral and bacterial pathogens seem to exploit this property and directly induce an immunosuppressive phenotype in T-cells by binding to CD46. -
组织特异性
Expressed by all cells except erythrocytes. -
疾病相关
Defects in CD46 are a cause of susceptibility to hemolytic uremic syndrome atypical type 2 (AHUS2) [MIM:612922]. An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Note=Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype. Patients with CD46 mutations seem to have an overall better prognosis compared to patients carrying CFH mutations. -
序列相似性
Contains 4 Sushi (CCP/SCR) domains. -
结构域
Sushi domains 1 and 2 are required for interaction with human adenovirus B PIV/FIBER protein and with Measles virus H protein. Sushi domains 2 and 3 are required for Herpesvirus 6 binding. Sushi domain 3 is required for Neisseria binding. Sushi domains 3 and 4 are required for interaction with Streptococcus pyogenes M protein and are the most important for interaction with C3b and C4b. -
翻译后修饰
N-glycosylated on Asn-83; Asn-114 and Asn-273 in most tissues, but probably less N-glycosylated in testis. N-glycosylation on Asn-114 and Asn-273 is required for cytoprotective function. N-glycosylation on Asn-114 is required for Measles virus binding. N-glycosylation on Asn-273 is required for Neisseria binding. N-glycosylation is not required for human adenovirus binding.
Extensively O-glycosylated in the Ser/Thr-rich domain. O-glycosylation is required for Neisseria binding but not for Measles virus or human adenovirus binding.
In epithelial cells, isoforms B/D/F/H/J/L/3 are phosphorylated by YES1 in response to infection by Neisseria gonorrhoeae; which promotes infectivity. In T-cells, these isoforms may be phosphorylated by Lck. -
细胞定位
Cytoplasmic vesicle > secretory vesicle > acrosome inner membrane. Inner acrosomal membrane of spermatozoa. Internalized upon binding of Measles virus, Herpesvirus 6 or Neisseria gonorrhoeae, which results in an increased susceptibility of infected cells to complement-mediated injury. In cancer cells or cells infected by Neisseria, shedding leads to a soluble peptide. - Information by UniProt
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数据库链接
- Entrez Gene: 4179 Human
- Omim: 120920 Human
- SwissProt: P15529 Human
- Unigene: 510402 Human
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别名
- AHUS2 antibody
- Antigen defined by monoclonal antibody TRA 2 10 antibody
- Antigen identified by monoclonal antibody TRA 2 10 antibody
see all
图片
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ab108307, at 1/500 dilution, staining CD46 in paraffin-embedded human kidney tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108307).
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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ab108307 showing positive staining in human normal placenta tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108307).
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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ab108307 showing positive staining in human normal uterus tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108307).
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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ab108307 showing positive staining in human thyroid gland carcinoma tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108307).
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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ab108307 showing positive staining in human colonic adenocarcinoma tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108307).
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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ab108307 showing positive staining in human breast carcinoma tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108307).
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
-
ab108307 showing positive staining in human normal breast tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108307).
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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This IHC data was generated using the same anti-CD46 antibody clone, EPR4014, in a different buffer formulation (cat# ab108307).
ab108307, at 1/500 dilution, staining CD46 in paraffin-embedded human tonsil tissue.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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This WB data was generated using the same anti-CD46 antibody clone, EPR4014, in a different buffer formulation (cat# ab108307).
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: CD46 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: K562 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab108307 observed at 50-70 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab108307 was shown to specifically react with when CD46 knockout samples were used. Wild-type and CD46 knockout samples were subjected to SDS-PAGE. ab108307 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
Certificate of Compliance
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