重组

重组Anti-CD45RA抗体[4KB5] (ab755)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [4KB5] to CD45RA
  • Suitable for: IHC-P, Flow Cyt
  • Reacts with: Human

概述

  • 产品名称

    Anti-CD45RA抗体[4KB5]
    参阅全部 CD45RA 一抗

  • 描述

    小鼠单克隆抗体[4KB5] to CD45RA

  • 宿主

    Mouse

  • 经测试应用

    适用于: IHC-P, Flow Cytmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Tissue, cells or virus. This information is considered to be commercially sensitive.

  • 阳性对照

    • IHC-P: Human tonsil tissue. Flow Cyt: Human peripheral blood mononuclear cells.

  • 常规说明

    This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

性能

应用

Our Abpromise guarantee covers the use of ab755 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 0.239 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt Use a concentration of 1.017 µg/ml.

靶标

  • 功能

    Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity.

  • 疾病相关

    Severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive
    Multiple sclerosis

  • 序列相似性

    Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 2 tyrosine-protein phosphatase domains.

  • 结构域

    The first PTPase domain interacts with SKAP1.

  • 翻译后修饰

    Heavily N- and O-glycosylated.

  • 细胞定位

    Membrane. Membrane raft. Colocalized with DPP4 in membrane rafts.
  • Information by UniProt

  • 数据库链接

    • 别名

      • B220 antibody
      • CD45 antibody
      • CD45 antigen antibody
      • CD45R antibody
      • EC 3.1.3.48 antibody
      • GP180 antibody
      • L-CA antibody
      • LCA antibody
      • Leukocyte common antigen antibody
      • Loc antibody
      • Ly-5 antibody
      • LY5 antibody
      • Ly5, homolog of antibody
      • Lymphocyte antigen 5 antibody
      • Lyt-4 antibody
      • Protein tyrosine phosphatase, receptor type C antibody
      • Protein tyrosine phosphatase, receptor type, C antibody
      • Protein tyrosine phosphatase, receptor type, c polypeptide antibody
      • Ptprc antibody
      • PTPRC_HUMAN antibody
      • Receptor-type tyrosine-protein phosphatase C antibody
      • RT7 antibody
      • T200 antibody
      • T200 glycoprotein antibody
      • T200 leukocyte common antigen antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45RA antibody [4KB5] (ab755)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45RA antibody [4KB5] (ab755)

      Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CD45RA with ab755 at 0.239µg/ml, followed by ready to use secondary. Positive staining on human tonsil tissue is observed. The section was incubated with ab755 for 10 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use secondary.

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Flow Cytometry - Anti-CD45RA antibody [4KB5] (ab755)
      Flow Cytometry - Anti-CD45RA antibody [4KB5] (ab755)

      Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) (right) labeling CD45RA with ab755 at 1.017µg/ml compared with mouse monoclonal IgG Isotype Control (left). Goat anti-mouse IgG (Alexa Fluor® 488) (ab150113) at 1/2000 dilution was used as the secondary antibody.

      Gated on viable cells.

    文献

    发表研究结果有使用 ab755？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab755 被引用在 1 文献中.

    • Li FJ  et al. A rapid translocation of CD45RO but not CD45RA to lipid rafts in IL-6-induced proliferation in myeloma. Blood 105:3295-302 (2005). PubMed: 15626731

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    Question

    ab755 - This antibody is a tissue culture supernatant but it has a concentration, can you tell me how you got this concentration? How do you know that it's accurate?   ab8260 what is the concentration of this product?

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    Answer

    The concentration of the current lot of ab8260 that we have in stock is 2.0 mg/ml. However, they also informed me that this concentration does vary from lot to lot. Additionally, they have also let me know that the way the concentration was determined for ab755 was through radio immunoassay. 

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    Question

    No problem, only a question; the ds says: use at a dilution of 1 / 10 - 1 / 25 for 30 - 60 mins (ABC method). What is this referring to (esp. the incubation time), The customer is using the DAKO ABC kit.

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    Answer

    Thank you for your enquiry. Ab755 has been tested for application in Immunohistochemistry, both formalin-fixed paraffin-embedded sections and frozen sections. It is recommended to use this antibody at a dilution of 1:10 - 1:25 in an ABC method and an incubation period or 30 minutes at room temperature is suggested. We have ABC Immunohistochemical Staining IHC protocols located in the protocol section of our website as well if your customer would like to refer to them.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com