Anti-CD44抗体(ab157107)
Key features and details
- Rabbit polyclonal to CD44
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
-
产品名称
Anti-CD44抗体
参阅全部 CD44 一抗 -
描述
兔多克隆抗体to CD44 -
宿主
Rabbit -
经测试应用
适用于: WB, IP, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Baboon, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan, Platypus -
免疫原
Synthetic peptide within Human CD44 aa 650-750. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P16070-1 -
阳性对照
- IHC-P: Human squamous cell carcinoma, lung cancer, skin, cervical cancer. Mouse spleen and plasmacytoma tissue; rat spleen tissue. WB: A549, HeLa, U2OS, TCMK-1, NIH/3T3 cells IP: HeLa and HAP1 cells.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.00
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate -
Concentration information loading...
-
纯度
Immunogen affinity purified -
纯化说明
ab157107 was affinity purified using an epitope specific to CD44 immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab157107于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (2) |
1/2000 - 1/10000. Predicted molecular weight: 81 kDa.
|
IP |
Use at 2-10 µg/mg of lysate.
|
|
IHC-P | (11) |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
说明 |
---|
WB
1/2000 - 1/10000. Predicted molecular weight: 81 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
-
功能
Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events. -
组织特异性
Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells. -
序列相似性
Contains 1 Link domain. -
结构域
The lectin-like LINK domain is responsible for hyaluronan binding. -
翻译后修饰
Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
N-glycosylated.
O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s).
Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672. -
细胞定位
Membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 101008690 Baboon
- Entrez Gene: 451122 Chimpanzee
- Entrez Gene: 281057 Cow
- Entrez Gene: 101867504 Cynomolgus monkey
- Entrez Gene: 403939 Dog
- Entrez Gene: 100034221 Horse
- Entrez Gene: 960 Human
- Entrez Gene: 12505 Mouse
see all -
别名
- LHR antibody
- BA-1 antibody
- CD 44 antibody
see all
图片
-
ab157107 was shown to react with CD44 in wild-type HAP1 cells in Western blot with loss of signal observed in a CD44 knockout cell line. Wild-type HAP1 and CD44 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab157107 overnight at 4 °C at a 1/2000 dilution. Blots were incubated with goat anti-rabbit HRP secondary antibodies at 0.2 μg/ml before imaging. These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.
-
Immunohistochemistry analysis (formalin-fixed, paraffin embedded sections) of mouse plasmacytoma tissue staining CD-44 with ab157107 at 1:5000 dilution.
-
Immunoprecipitation of CD44 in HAP1 cells. Lysates were prepared and immunoprecipitation was performed using 1.0 μg of ab157107 pre-coupled to prot.A-Sepharose beads. Samples were washed and processed for western blot with ab254530 at 1/2000. This data was kindly provided by the YCharOS Inc., an open science company with the mission of characterizing every commercially available antibody reagent. Abcam are working with YCharOS to support their mission of antibody characterisation using knock out cell lines.
-
Immunoprecipitation of CD44 in HAP1 cells. Lysates were prepared and immunoprecipitation was performed using 1.0 μg of ab157107 pre-coupled to prot.A-Sepharose beads. Samples were washed and processed for western blot with ab254530 at 1/2000. SM=10% starting material; UB=10% unbound fraction; IP=immunoprecipitate. These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.
-
Immunohistochemistry analysis (formalin-fixed, paraffin embedded sections) of human lung cancer tissue staining CD-44 with ab157107 at 1:5000 dilution.
-
Immunohistochemistry analysis (formalin-fixed, paraffin embedded sections) of human head and neck squamous cell carcinoma tissue staining CD-44 with ab157107 at 1:5000 dilution.
-
Immunoprecipitatgion of HeLa cells with:
Lane 1: Rabbit anti-CD44 recombinant monoclonal antibody
Lane 2: ab157107 lot A303-872A-3
Lane 3: ab157107 lot A303-872A-4
Lane 4: Control IgG
-
All lanes : Anti-CD44 antibody (ab157107) at 0.1 µg/ml
Lane 1 : TMCK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 2 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 81 kDa
Exposure time: 30 seconds -
All lanes : Anti-CD44 antibody (ab157107) at 0.04 µg/ml
Lane 1 : A-549 (human lung carcinoma cell line) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : U20S (human bone osteosarcoma epithelial cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 81 kDa
Exposure time: 30 seconds
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (172)
ab157107 被引用在 172 文献中.
- Zhang P et al. Dual-stimuli responsive smart nanoprobe for precise diagnosis and synergistic multi-modalities therapy of superficial squamous cell carcinoma. J Nanobiotechnology 21:4 (2023). PubMed: 36597067
- García-Gómez P et al. NOX4 regulates TGFβ-induced proliferation and self-renewal in glioblastoma stem cells. Mol Oncol 16:1891-1912 (2022). PubMed: 35203105
- Zhao J et al. Bone Regeneration and Angiogenesis by Co-transplantation of Angiotensin II-Pretreated Mesenchymal Stem Cells and Endothelial Cells in Early Steroid-Induced Osteonecrosis of the Femoral Head. Cell Transplant 31:9636897221086965 (2022). PubMed: 35313737
- Colapietro A et al. The Botanical Drug PBI-05204, a Supercritical CO2 Extract of Nerium Oleander, Is Synergistic With Radiotherapy in Models of Human Glioblastoma. Front Pharmacol 13:852941 (2022). PubMed: 35401175
- Cui J et al. Biomechanically and biochemically functional scaffold for recruitment of endogenous stem cells to promote tendon regeneration. NPJ Regen Med 7:26 (2022). PubMed: 35474221