Anti-TRAP/CD40L抗体[MR-1] - Low endotoxin,Azide free (ab99895)
Key features and details
- Armenian hamster monoclonal [MR-1] to TRAP/CD40L - Low endotoxin, Azide free
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Isotype: IgG1
Related conjugates and formulations
概述
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产品名称
Anti-TRAP/CD40L抗体[MR-1] - Low endotoxin,Azide free
参阅全部 TRAP/CD40L 一抗 -
描述
亚美尼亚仓鼠单克隆抗体[MR-1] to TRAP/CD40L - Low endotoxin,Azide free -
宿主
Armenian hamster -
经测试应用
适用于: Flow Cytmore details -
种属反应性
与反应: Mouse -
免疫原
Tissue, cells or virus corresponding to Mouse TRAP/CD40L. Activated Mouse Th1 clone D1.6
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阳性对照
- 6-8 hour activated mouse splenocytes
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.4 -
无载体
是 -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
MR-1 -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab99895于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt |
Use a concentration of 4 µg/ml.
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说明 |
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Flow Cyt
Use a concentration of 4 µg/ml. |
靶标
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功能
Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching.
Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway. -
组织特异性
Specifically expressed on activated CD4+ T-lymphocytes. -
疾病相关
Defects in CD40LG are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1) [MIM:308230]; also known as X-linked hyper IgM syndrome (XHIM). HIGM1 is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence. -
序列相似性
Belongs to the tumor necrosis factor family. -
翻译后修饰
The soluble form derives from the membrane form by proteolytic processing.
N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40.
Not O-glycosylated. -
细胞定位
Secreted and Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 21947 Mouse
- SwissProt: P27548 Mouse
- Unigene: 4861 Mouse
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别名
- CD 40L antibody
- CD154 antibody
- CD40 antigen ligand antibody
see all
图片
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Separation of CD154 positive cells (red-filled) from CD154 negative cells (black-dashed) in flow cytometry analysis (surface staining) of murine PMA, ionomycin and LPS stimulated splenocytes stained using ab99895 at 3 μg/ml
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Flow cytometry surface staining pattern of murine PMA, ionomycin and LPS stimulated splenocytes stained using ab99895 at 3 μg/ml
实验方案
数据表及文件
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Datasheet download
文献 (5)
ab99895 被引用在 5 文献中.
- Hwang Y et al. Acquired, but not innate, immune responses to Streptococcus pneumoniae are compromised by neutralization of CD40L. Infect Immun 68:511-7 (2000). PubMed: 10639411
- Lode HN et al. Melanoma immunotherapy by targeted IL-2 depends on CD4(+) T-cell help mediated by CD40/CD40L interaction. J Clin Invest 105:1623-30 (2000). PubMed: 10841521
- Howard LM et al. Mechanisms of immunotherapeutic intervention by anti-CD40L (CD154) antibody in an animal model of multiple sclerosis. J Clin Invest 103:281-90 (1999). PubMed: 9916140
- Salgado CG et al. Functional CD40 ligand is expressed on epidermal Langerhans cells. J Leukoc Biol 66:281-5 (1999). PubMed: 10449168
- Noelle RJ et al. A 39-kDa protein on activated helper T cells binds CD40 and transduces the signal for cognate activation of B cells. Proc Natl Acad Sci U S A 89:6550-4 (1992). PubMed: 1378631