Anti-CD4抗体[W3/25] (ab6413)

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Flow Cytometry - Anti-CD4 antibody [W3/25] (ab6413)

    Key features and details

    • Mouse monoclonal [W3/25] to CD4
    • Suitable for: Flow Cyt
    • Reacts with: Rat
    • Isotype: IgG1

    概述

    • 产品名称

      Anti-CD4抗体[W3/25]
      参阅全部 CD4 一抗

    • 描述

      小鼠单克隆抗体[W3/25] to CD4

    • 宿主

      Mouse

    • 经测试应用

      适用于: Flow Cytmore details
      不适用于: IHC-P

    • 种属反应性

      与反应: Rat

    • 免疫原

      Tissue, cells or virus corresponding to Rat CD4. Rat thymocyte membrane.

    • 阳性对照

      • Flow cyt: Lewis rat splenocytes.

    • 常规说明

      This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

       

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    性能

    应用

    Our Abpromise guarantee covers the use of ab6413 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    Flow Cyt Use a concentration of 0.2 µg/ml.
  • 应用说明
    Is unsuitable for IHC-P.

    • 靶标

    • 功能

      Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.

    • 序列相似性

      Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.

    • 翻译后修饰

      Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts.

    • 细胞定位

      Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
    • Information by UniProt

    • 数据库链接

      • 别名

        • CD 4 antibody
        • CD4 (L3T4) antibody
        • CD4 antibody
        • CD4 antigen (p55) antibody
        • CD4 antigen antibody
        • CD4 molecule antibody
        • CD4 receptor antibody
        • CD4+ Lymphocyte deficiency, included antibody
        • CD4_HUMAN antibody
        • CD4mut antibody
        • L3T4 antibody
        • Leu3 antibody
        • Ly-4 antibody
        • Lymphocyte antigen CD4 antibody
        • MGC165891 antibody
        • OTTHUMP00000238897 antibody
        • p55 antibody
        • T cell antigen T4 antibody
        • T cell antigen T4/LEU3 antibody
        • T cell differentiation antigen L3T4 antibody
        • T cell OKT4 deficiency, included antibody
        • T cell surface antigen T4/Leu 3 antibody
        • T cell surface antigen T4/Leu3 antibody
        • T cell surface glycoprotein CD4 antibody
        • T-cell surface antigen T4/Leu-3 antibody
        • T-cell surface glycoprotein CD4 antibody
        • W3/25 antibody
        • W3/25 antigen antibody
        see all

      图片

      • Flow Cytometry - Anti-CD4 antibody [W3/25] (ab6413)
        Flow Cytometry - Anti-CD4 antibody [W3/25] (ab6413)

        Lewis rat splenocytes stained with ab6413 (right) or mouse IgG1κ (ab170190) isotype (left). Lewis rat splenocytes were incubated for 30 min on ice in 1x PBS / 10 % rat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab6413) or mouse IgG1κ (ab170190) (1x106 in 100 µl at 0.2 µg/ml) for 30 min on ice.

        The secondary antibody Goat anti-mouse IgG H&L (Alexa Fluor ® 488, pre-adsorbed) (ab150117) was used at 1/2000 dilution for 30 min on ice. The cells were simultaneously stained with CD3 antibody.

        Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on viable lymphocytes.

         

      实验方案

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      文献 (5)

      发表研究结果有使用 ab6413？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab6413 被引用在 5 文献中.

      • Grémy O  et al. Caffeic acid phenethyl ester modifies the Th1/Th2 balance in ileal mucosa after gamma-irradiation in the rat by modulating the cytokine pattern. World J Gastroenterol 12:4996-5004 (2006). PubMed: 16937495
      • Jefferies WA  et al. Authentic T helper CD4 (W3/25) antigen on rat peritoneal macrophages. J Exp Med 162:117-27 (1985). PubMed: 3159821
      • Barclay AN The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues. Immunology 42:593-600 (1981). PubMed: 7016746
      • Webb M  et al. Inhibition of mixed lymphocyte response by monoclonal antibody specific for a rat T lymphocyte subset. Nature 282:841-3 (1979). PubMed: 160013
      • White RA  et al. T-lymphocyte heterogeneity in the rat: separation of functional subpopulations using a monoclonal antibody. J Exp Med 148:664-73 (1978). PubMed: 29936

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      Question

      ab755 is sorted out now. Thanks for this, customer is satisfied. ab6413: they formalin fixed paraffin tissue. According to ds the dilution should be 1:400. Which incubation conditions would you recommend (time, temp:

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      Answer

      Regarding the use of ab6413 in IHC-formalin fixed paraffin embedded sections, a 1:400 dilution is recommended and I suggest incubating overnight at 4 C. Please have your customer refer to our IHC-Paraffin sections protocol which can be found on our website (click on the protocols tab located on the online datasheet for ab6413). Also, your customer may want to take a look at the following publications which have used this antibody: Jefferies WA et al. Authentic T helper CD4 (W3/25) antigen on rat peritoneal macrophages. J Exp Med 162:117-27 (1985). PubMed: 3159821 Barclay AN The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues. Immunology 42:593-600 (1981). PubMed: 7016746 Webb M et al. Inhibition of mixed lymphocyte response by monoclonal antibody specific for a rat T lymphocyte subset. Nature 282:841-3 (1979). PubMed: 160013 White RA et al. T-lymphocyte heterogeneity in the rat: separation of functional subpopulations using a monoclonal antibody. J Exp Med 148:664-73 (1978). PubMed: 29936

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      Question

      Hi!I am in Beijing, China.about your product CD4 antibody (ab6413) [W3/25].Is it fit for Frozen tissure? As I am intending to purchase such kind of product, do you have any in small size? looking forward to your reply!Thank you!

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      Answer

      This antibody has not been tested in frozen tissue sections so we are not aware of its suitability. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you decide to test an antibody in an application for which we do not have any information, please let us know how you get on and in return we will forward a GBP10/ USD15/ EUR15 Amazon gift voucher.

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      Question

      I purchased antibody to label active cells in lymphoid tissues (spleen, thymus, MLN). Used 5um sections of 10% NBF for 24 hr tissues routinely processed to paraffin. I followed your online IHC protocol for paraffin sections. I used the AR protocol for the pressure cooker. I ran the antibody at 1/50, 1/200 and 1/400. The secondary antibody was anti-Mouse antibody was from Vector diluted 1/150. The negative control was completely absent of staining as expected. However, the test slides were also negative except for three or four cells that had cytoplasmic staining, that looked liked dead cell debris. I am very optimisitic about purchasing since you are the only company that I could find that sells an anti-rat CD4 that claims to work on formalin fixed paraffin sections. Many thanks,

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      Answer

      This antibody has ben tested on paraffin sections so it should work nicely. Please have a look at the datasheet for references on staining and preparation of sections.

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