Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (B16F10 (melanoma) syngeneic mouse tumor)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA
Permeabilization
Yes - 3% H2O2
Specification
B16F10 (melanoma) syngeneic mouse tumor
Fixative
Formaldehyde
Other product details
Incubation time
36 minute(s) · Temperature: 37°C
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: Anti-Rb-HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
The secondary antibody was incubated for 16 min.
The specific membranous pattern of helper T cell was well distinguished.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Apr 30 2019