重组Anti-CD3G抗体[EPR4517] (ab134096)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4517] to CD3G
- Suitable for: WB, IP, IHC-P, Flow Cyt (Intra), ICC/IF
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-CD3G抗体[EPR4517]
参阅全部 CD3G 一抗 -
描述
兔单克隆抗体[EPR4517] to CD3G -
宿主
Rabbit -
经测试应用
适用于: WB, IP, IHC-P, Flow Cyt (Intra), ICC/IFmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide within Human CD3G (C terminal). The exact sequence is proprietary.
Database link: P09693 -
阳性对照
- WB: Jurkat and mouse thymus lysates. IHC-P: Human tonsil, human colon and mouse spleen tissues. ICC/IF: Jurkat cells. IP: Jurkat cell lysate. Flow Cyt (intra): Jurkat cells
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR4517 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab134096于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/20000 - 1/200000. Predicted molecular weight: 20 kDa.
For unpurified use at 1/10000 - 1/50000. |
|
IP |
1/40.
For unpurified use at 1/10 - 1/100. |
|
IHC-P |
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
For unpurified use at 1/250 - 1/500. |
|
Flow Cyt (Intra) |
1/70.
|
|
ICC/IF |
1/1000.
For unpurified use at 1/500 - 1/1000. |
说明 |
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WB
1/20000 - 1/200000. Predicted molecular weight: 20 kDa. For unpurified use at 1/10000 - 1/50000. |
IP
1/40. For unpurified use at 1/10 - 1/100. |
IHC-P
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. For unpurified use at 1/250 - 1/500. |
Flow Cyt (Intra)
1/70. |
ICC/IF
1/1000. For unpurified use at 1/500 - 1/1000. |
靶标
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功能
The CD3 complex mediates signal transduction. -
序列相似性
Contains 1 Ig-like (immunoglobulin-like) domain.
Contains 1 ITAM domain. -
结构域
A di-leucine motif and a tyrosine-based motif are individually sufficient to induce both endocytosis and delivery to lysosomes. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 917 Human
- Entrez Gene: 12502 Mouse
- Omim: 186740 Human
- SwissProt: P09693 Human
- SwissProt: P11942 Mouse
- Unigene: 2259 Human
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别名
- CD3 gamma antibody
- CD3-GAMMA antibody
- CD3g antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling CD3G with purified ab134096 at 1/1000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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All lanes : Anti-CD3G antibody [EPR4517] (ab134096) at 1/50000 dilution (purified)
Lane 1 : Jurkat cell lysate
Lane 2 : Human fetal thymus lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 20 kDa
Observed band size: 18-28 kDa why is the actual band size different from the predicted?Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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Immunocytochemistry/Immunofluorescence analysis of Jurkat cells labelling CD3G with purified ab134096 at 1/1000. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. An Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.
Control: primary antibody (1/1000) and secondary antibody, ab150113, an Alexa Fluor® 488-conjugated goat anti-mouse IgG (1/500).
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Intracellular Flow Cytometry analysis of Jurkat (human acute T cell leukemia) cells labeling CD3G with purified ab134096 at 1/70 dilution (10ug/mL) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor®488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.
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Anti-CD3G antibody [EPR4517] (ab134096) at 1/50000 dilution (purified) + Mouse thymus tissue lysate at 20 µg
Secondary
Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 20 kDa
Observed band size: 18-28 kDa why is the actual band size different from the predicted?Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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All lanes : Anti-CD3G antibody [EPR4517] (ab134096) at 1/10000 dilution (unpurified)
Lane 1 : Jurkat cell lysate
Lane 2 : Mouse thymus tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 20 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling CD3G with unpurified ab134096.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse spleen tissue labelling CD3G with unpurified ab134096.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon (lymphocytes) tissue labelling CD3G with unpurified ab134096.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed parffin-embedded sections) analysis of human skeletal muscle tissue. Unpurified ab32362 shows negative staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed parffin-embedded sections) analysis of human brain tissue. Unpurified ab32362 shows negative staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ab134096 (purified) at 1/40 immunoprecipitating CD3G in Jurkat cell lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (19)
ab134096 被引用在 19 文献中.
- An C et al. Myeloid PTEN deficiency aggravates renal inflammation and fibrosis in angiotensin II-induced hypertension. J Cell Physiol 237:983-991 (2022). PubMed: 34515350
- Martin-Jaular L et al. Unbiased proteomic profiling of host cell extracellular vesicle composition and dynamics upon HIV-1 infection. EMBO J 40:e105492 (2021). PubMed: 33709510
- Manils J et al. CARD14E138A signalling in keratinocytes induces TNF-dependent skin and systemic inflammation. Elife 9:N/A (2020). PubMed: 32597759
- Monin L et al. ?d T cells compose a developmentally regulated intrauterine population and protect against vaginal candidiasis. Mucosal Immunol 13:969-981 (2020). PubMed: 32472066
- Moreira-Teixeira L et al. Mouse transcriptome reveals potential signatures of protection and pathogenesis in human tuberculosis. Nat Immunol 21:464-476 (2020). PubMed: 32205882