重组Anti-CD39抗体[EPR3678(2)] (ab108248)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3678(2)] to CD39
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-CD39抗体[EPR3678(2)]
参阅全部 CD39 一抗 -
描述
兔单克隆抗体[EPR3678(2)] to CD39 -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF,IHC-P or IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Human placenta, rat hippocampus, rat pancreas, mouse placenta and mouse urinary bladder tissue lysates; IM-9 and HUVEC cell lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR3678(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab108248于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 58 kDa.
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说明 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 58 kDa. |
靶标
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功能
In the nervous system, could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. Could also be implicated in the prevention of platelet aggregation. Hydrolyzes ATP and ADP equally well. -
组织特异性
Expressed primarily on activated lymphoid cells. Also expressed in endothelial tissues. The vascular isoform and the placental isoform II are present in both placenta and umbilical vein, whereas placental isoform I is present in placenta only. -
序列相似性
Belongs to the GDA1/CD39 NTPase family. -
翻译后修饰
The N-terminus is blocked.
Palmitoylated in the N-terminal part. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 953 Human
- Entrez Gene: 12495 Mouse
- Entrez Gene: 64519 Rat
- Omim: 601752 Human
- SwissProt: P49961 Human
- SwissProt: P55772 Mouse
- SwissProt: P97687 Rat
- Unigene: 576612 Human
see all -
形式
There are 3 isoforms produced by alternative splicing. -
别名
- ATPDase antibody
- CD 39 antibody
- CD39 antibody
see all
图片
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All lanes : Anti-CD39 antibody [EPR3678(2)] (ab108248) at 1/1000 dilution (purified)
Lane 1 : Rat hippocampus tissue lysate
Lane 2 : Rat pancreas tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 58 kDa
Observed band size: 70,74 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration 5% NFDM/TBST.
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All lanes : Anti-CD39 antibody [EPR3678(2)] (ab108248) at 1/1000 dilution (purified)
Lane 1 : Mouse placenta tissue lysate
Lane 2 : Mouse urinary bladder tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 58 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsBlocking and diluting buffer and concentration 5% NFDM/TBST.
The PKC beta 2 recombinant protein is GST-tagged, so the molecular weight is higher.
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Lane 1 : Anti-CD39 antibody [EPR3678(2)] (ab108248) at 1/100 dilution (purified)
Lane 2 : Anti-CD39 antibody [EPR3678(2)] (ab108248) at 1/1000 dilution (purified)
All lanes : HUVEC whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 58 kDa
Observed band size: 70,74 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and diluting buffer and concentration 5% NFDM/TBST.
Ref: PMID: 18693757
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All lanes : Anti-CD39 antibody [EPR3678(2)] (ab108248) at 1/1000 dilution (unpurified)
Lane 1 : Human placenta lysate
Lane 2 : IM-9 cell lysate
Predicted band size: 58 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (17)
ab108248 被引用在 17 文献中.
- Zhou X et al. Electroacupuncture relieves neuropathic pain by inhibiting degradation of the ecto-nucleotidase PAP in the dorsal root ganglions of CCI mice. Eur J Pain 26:991-1005 (2022). PubMed: 35138669
- Joung J et al. CRISPR activation screen identifies BCL-2 proteins and B3GNT2 as drivers of cancer resistance to T cell-mediated cytotoxicity. Nat Commun 13:1606 (2022). PubMed: 35338135
- Calame DG et al. Biallelic Variants in the Ectonucleotidase ENTPD1 Cause a Complex Neurodevelopmental Disorder with Intellectual Disability, Distinct White Matter Abnormalities, and Spastic Paraplegia. Ann Neurol 92:304-321 (2022). PubMed: 35471564
- Macedo C et al. Donor-derived regulatory dendritic cell infusion results in host cell cross-dressing and T cell subset changes in prospective living donor liver transplant recipients. Am J Transplant 21:2372-2386 (2021). PubMed: 33171019
- Hamed FN et al. Alopecia areata patients show deficiency of FOXP3+CD39+ T regulatory cells and clonotypic restriction of Treg TCRß-chain, which highlights the immunopathological aspect of the disease. PLoS One 14:e0210308 (2019). PubMed: 31277078