Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa, Mel202, A431, HCT116, JURKAT)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
HeLa, Mel202, A431, HCT116, JURKAT
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk
Dilution
1/1000
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
SuperSignal West Pico Chemiluminescent S
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 53, 80 kDa Non-specific: 120-180 kDa
Additional data
Additional Notes
Lane 1- HeLa, Lane 2- Mel202, Lane 3- A431, Lane 4- HCT116, Lane 5- JURKAT
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jul 26 2016