Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 1.5% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: antigen retrieval
Sample
Mouse Tissue sections (Heart at day 0 and day 7 post-MI)
Specification
Heart at day 0 and day 7 post-MI
Permeabilization
No
Fixative
10% zinc formalin
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: normal goat serum
Dilution
1/100
Secondary antibody
Name
Non-Abcam antibody was used: biotinylated goat anti-rabbit IgG secondary antibo
Host species: Goat
Clonality: Monoclonal
Conjugation: Biotin
Host species: Goat
Clonality: Monoclonal
Conjugation: Biotin
Dilution
1/200
Additional data
Additional Notes
Tissue fixed in 10% zinc formalin
Heat mediated antigen retrieval performed using Target Retrieval System, 10X (Dako S1699)
Antibody diluted 1:100 in blocking serum
No specific staining seen under these conditions
Abcam response
Thank you for your invaluable data which suggest that ab133625 may not be suitable for use in IHC. Please note that IHC is not listed as a tested application on the product datasheet.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Merry Lindsey
Verified customer
提交于 Oct 17 2013