Anti-CD35抗体[E11] - BSA and Azide free (ab25)
Key features and details
- Mouse monoclonal [E11] to CD35 - BSA and Azide free
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-CD35抗体[E11] - BSA and Azide free
参阅全部 CD35 一抗 -
描述
小鼠单克隆抗体[E11] to CD35 - BSA and Azide free -
宿主
Mouse -
特异性
Does not block complement receptor 1 (CR1) activity. No cross reaction with CR2. -
Tested Applications & Species
Application Species IHC-P Human -
免疫原
Tissue, cells or virus corresponding to CD35. human acute monocytic leukaemia cells.
Database link: P17927
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Constituent: PBS -
无载体
是 -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
E11 -
骨髓瘤
P3-NS1/1-Ag4-1 -
同种型
IgG -
轻链类型
unknown -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
应用
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab25 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
应用 | Species |
---|---|
IHC-P |
Human
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应用 | Ab评论 | 说明 |
---|---|---|
IHC-P |
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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IHC-P
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Mediates cellular binding of particles and immune complexes that have activated complement. -
组织特异性
Present on erythrocytes, leukocytes, glomerular podocytes, and splenic follicular dendritic cells. -
序列相似性
Belongs to the receptors of complement activation (RCA) family.
Contains 30 Sushi (CCP/SCR) domains. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 1378 Human
- Omim: 120620 Human
- SwissProt: P17927 Human
- Unigene: 334019 Human
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别名
- C3 binding protein antibody
- C3b/C4b receptor antibody
- C3BR antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD35 [E11] antibody - BSA and Azide free (ab25)ab25 (1µg/ml) staining CD35 in human spleen.
Sections were stained using an automated system (DAKO Autostainer Plus ), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab25 被引用在 6 文献中.
- Yuan K et al. Complement C3 overexpression activates JAK2/STAT3 pathway and correlates with gastric cancer progression. J Exp Clin Cancer Res 39:9 (2020). PubMed: 31928530
- Fonseca MI et al. Analysis of the Putative Role of CR1 in Alzheimer's Disease: Genetic Association, Expression and Function. PLoS One 11:e0149792 (2016). PubMed: 26914463
- Fetica B et al. High prevalence of viral hepatitis in a series of splenic marginal zone lymphomas from Romania. Blood Cancer J 6:e498 (2016). PubMed: 27834940
- Park HJ et al. Using mutagenesis and structural biology to map the binding site for the Plasmodium falciparum merozoite protein PfRh4 on the human immune adherence receptor. J Biol Chem 289:450-63 (2014). ELISA . PubMed: 24214979
- LaPan P et al. Optimization of total protein and activity assays for the detection of MMP-12 in induced human sputum. BMC Pulm Med 10:40 (2010). ELISA ; Human . PubMed: 20678199
- Hogg N et al. Identification of an anti-monocyte monoclonal antibody that is specific for membrane complement receptor type one (CR1). Eur J Immunol 14:236-43 (1984). PubMed: 6368248