Anti-CD34抗体[QBEND-10] (ab8536)
Key features and details
- Mouse monoclonal [QBEND-10] to CD34
- Suitable for: Flow Cyt, IHC-P, WB
- Reacts with: Human
- Isotype: IgG1
Related conjugates and formulations
概述
-
产品名称
Anti-CD34抗体[QBEND-10]
参阅全部 CD34 一抗 -
描述
小鼠单克隆抗体[QBEND-10] to CD34 -
宿主
Mouse -
特异性
This antibody recognizes the CD34 antigen, some cross reactions with vascular associated adventitia and some basement membranes. Ultrastructure studies, using an indirect immuno-gold technique on ultra thin sections of human umbilical artery, have revealed that this antigen is associated to a degree with the basoluminal surfaces of endothelium and shows a slight reaction with an intracytoplasmic vesicular compartment. Predominant staining is on the endothelial cell membrane. QBEND-10 immunoprecipitates a glycoprotein with a relative MW 110kD expressed on haematopoietic cells and on the established myeloid leukaemic cell line KG1A. -
经测试应用
适用于: Flow Cyt, IHC-P, WBmore details -
种属反应性
与反应: Human
不与反应: Rat, Sheep, Cow, Dog -
免疫原
Tissue, cells or virus corresponding to Human CD34. Human endothelial vesicles
Database link: P28906 -
表位
This antibody's target epitope is distinct from those of other anti-CD34 reactive mabs as demonstrated by its lack of sensitivity to neuraminidase treatment, and fluorescence staining profiles during flow cytometry analysis of target cell populations. -
阳性对照
- tonsil, bone marrow
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
-
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
QBEND-10 -
骨髓瘤
unknown -
同种型
IgG1 -
轻链类型
unknown -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt |
1/50.
Mmethanol fixed cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
IHC-P | (4) |
1/50 - 1/100. Do not perform antigen retrieval.
|
WB |
Use at an assay dependent concentration. Predicted molecular weight: 40 kDa.
|
说明 |
---|
Flow Cyt
1/50. Mmethanol fixed cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
IHC-P
1/50 - 1/100. Do not perform antigen retrieval. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 40 kDa. |
靶标
-
功能
Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins. -
组织特异性
Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues. -
序列相似性
Belongs to the CD34 family. -
发展阶段
On early hematopoietic progenitor cells. -
翻译后修饰
Highly glycosylated.
Phosphorylated on serine residues by PKC. -
细胞定位
Membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 947 Human
- Omim: 142230 Human
- SwissProt: P28906 Human
- Unigene: 374990 Human
-
别名
- CD34 antibody
- CD34 antigen antibody
- CD34 molecule antibody
see all
图片
-
Overlay histogram showing Jurkat cells stained with ab8536 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8536, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a significantly decreased signal in Jurkat cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.
Please note that Abcam do not have data for use of this antibody on non-fixed cells. We welcome any customer feedback. -
Anti-CD34 antibody [QBEND-10] (ab8536) at 5 µg/ml + Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 40 kDa
Observed band size: 40 kDa
Additional bands at: 100 kDa (possible glycosylated form) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue sections labelling CD34 with ab8536 at 1µg/ml (1:1000). Blood vessels show positive staining.
Detection system polymeric HRP. HIER with Pressure cooker pH6.
-
ab8536 staining CD34 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 2% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/2000 in blocking buffer) for 16 hours at 21°C. A Biotin-conjugated goat anti-mouse IgG polyclonal (1/300) was used as the secondary antibody.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue sections labelling CD34 with ab8536 at 1µg/ml (1:1000). Blood vessels show positive staining.
Detection system polymeric HRP. HIER with Pressure cooker pH6.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue sections labeling CD34 with ab8536 at 1ug/ml.
Detection system polymeric HRP. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human umbilical cord labeling CD34 with ab8536 at 1/1000 dilution. Tissue was fixed in formaldehyde and permeabilized with PBS. Heat mediated antigen retrieval was performed using a citrate pH 6 buffer. Tissue was blocked with 5% serum for 1 hour at room temperature. A biotinylated polyclonal goat anti-mouse secondary antibody was used at 1/100 dilution.
-
ab8536 at 1/500 staining human umbilical cord tissue sections by IHC-P. The tissue was paraformaldehyde fixed and blocked with horse serum before incubation with the antibody for 16 hours. A biotinylated goat anti-mouse IgG was used as the secondary.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (62)
ab8536 被引用在 62 文献中.
- Anderson AE et al. An immunologically active, adipose-derived extracellular matrix biomaterial for soft tissue reconstruction: concept to clinical trial. NPJ Regen Med 7:6 (2022). PubMed: 35031598
- Jin Y et al. Development of STEAP1 targeting chimeric antigen receptor for adoptive cell therapy against cancer. Mol Ther Oncolytics 26:189-206 (2022). PubMed: 35860008
- Han X et al. Basal microvilli define the metabolic capacity and lethal phenotype of pancreatic cancer. J Pathol 253:304-314 (2021). PubMed: 33159698
- Heylmann D et al. Comparison of DNA repair and radiosensitivity of different blood cell populations. Sci Rep 11:2478 (2021). PubMed: 33510180
- Koo HS et al. Non-invasive Intrauterine Administration of Botulinum Toxin A Enhances Endometrial Angiogenesis and Improves the Rates of Embryo Implantation. Reprod Sci 28:1671-1687 (2021). PubMed: 33650094