Anti-CD34抗体[4H11(APG)] (ab762)
Key features and details
- Mouse monoclonal [4H11(APG)] to CD34
- Suitable for: Flow Cyt, IHC-P
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-CD34抗体[4H11(APG)]
参阅全部 CD34 一抗 -
描述
小鼠单克隆抗体[4H11(APG)] to CD34 -
宿主
Mouse -
特异性
This antibody reacts with Class III epitope on CD34 antigen. -
Tested Applications & Species
Application Species IHC-P HumanWB Recombinant fragment -
免疫原
Tissue/ cell preparation (Human). (Permanent human cell line derived from peripheral leucocytes of a patient suffering from chronic myeloid leukaemia).
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阳性对照
- This antibody gave a positive result in IHC in the following FFPE tissue: Human normal placenta.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Proprietary Purification -
纯化说明
Purified from TCS. Purified by precipitation and chromatography. Purity >95% by SDS-PAGE. -
克隆
单克隆 -
克隆编号
4H11(APG) -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab762 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
应用 | Species |
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IHC-P |
Human
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WB |
Recombinant fragment
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应用 | Ab评论 | 说明 |
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Flow Cyt |
Use a concentration of 2 µg/ml.
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IHC-P |
Use a concentration of 10 µg/ml.
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说明 |
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Flow Cyt
Use a concentration of 2 µg/ml. |
IHC-P
Use a concentration of 10 µg/ml. |
靶标
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功能
Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins. -
组织特异性
Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues. -
序列相似性
Belongs to the CD34 family. -
发展阶段
On early hematopoietic progenitor cells. -
翻译后修饰
Highly glycosylated.
Phosphorylated on serine residues by PKC. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 947 Human
- Omim: 142230 Human
- SwissProt: P28906 Human
- Unigene: 374990 Human
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别名
- CD34 antibody
- CD34 antigen antibody
- CD34 molecule antibody
see all
图片
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Flow cytometric surface staining pattern showing CD34 positive stem cells in red stained with 2µg/ml ab762 in human peripheral whole blood.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [4H11(APG)] (ab762)
IHC image of CD34 staining in Human normal placenta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab762, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab762 被引用在 2 文献中.
- Nies C et al. A Microcavity Array-Based 4D Cell Culture Platform. Bioengineering (Basel) 6:N/A (2019). PubMed: 31159244
- Mattheij NJ et al. Dual mechanism of integrin aIIbß3 closure in procoagulant platelets. J Biol Chem 288:13325-36 (2013). PubMed: 23519467