- 数据表
- 具体的参考文献 (1)
- 实验方案
概述
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产品名称Anti-CD34抗体[4H11(APG)]
参阅全部 CD34 一抗 -
描述小鼠单克隆抗体[4H11(APG)] to CD34
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宿主Mouse
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特异性This antibody reacts with Class III epitope on CD34 antigen.
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经测试应用适用于: IHC-P, WB, Flow Cytmore details
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种属反应性与反应: Human
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免疫原
Tissue/ cell preparation (Human). (Permanent human cell line derived from peripheral leucocytes of a patient suffering from chronic myeloid leukaemia).
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阳性对照
- This antibody gave a positive result in IHC in the following FFPE tissue: Human normal placenta.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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存储溶液pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度>95% by SDS-PAGE
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纯化说明Purified from TCS
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克隆单克隆
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克隆编号4H11(APG)
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同种型IgG1
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研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab762 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | Use a concentration of 10 µg/ml. | |
| WB | Use a concentration of 2 µg/ml. Use under non reducing condition. Predicted molecular weight: 115 kDa. | |
| Flow Cyt | Use a concentration of 2 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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靶标
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功能Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
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组织特异性Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.
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序列相似性Belongs to the CD34 family.
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发展阶段On early hematopoietic progenitor cells.
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翻译后修饰Highly glycosylated.
Phosphorylated on serine residues by PKC. -
细胞定位Membrane.
- Information by UniProt
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数据库链接
- Entrez Gene: 947 Human
- Omim: 142230 Human
- SwissProt: P28906 Human
- Unigene: 374990 Human
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别名
- CD34 antibody
- CD34 antigen antibody
- CD34 molecule antibody
see all
图片
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [4H11(APG)] (ab762)](https://www.abcam.cn/ps/datasheet/images/0/ab762/CD34-Primary-antibodies-ab762-1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [4H11(APG)] (ab762)IHC image of CD34 staining in Human normal placenta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab762, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
文献
This product has been referenced in:
- Mattheij NJ et al. Dual mechanism of integrin aIIbß3 closure in procoagulant platelets. J Biol Chem 288:13325-36 (2013). Read more (PubMed: 23519467) »
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"