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Cardiovascular Blood Platelets
重组

重组Anti-CD31抗体[WM59] (ab218)

  • Datasheet
  • SDS
Reviews (1)Q&A (2)References (6)

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Immunocytochemistry - Anti-CD31 antibody [WM59] (ab218)
  • Flow Cytometry - Anti-CD31 antibody [WM59] (ab218)
  • Anti-CD31 antibody [WM59] (ab218)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [WM59] to CD31
  • Suitable for: Flow Cyt, ICC
  • Reacts with: Human

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概述

  • 产品名称

    Anti-CD31抗体[WM59]
    参阅全部 CD31 一抗
  • 描述

    小鼠单克隆抗体[WM59] to CD31
  • 宿主

    Mouse
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC
    Human
    See all applications and species data
  • 免疫原

    Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • ICC: HUVEC cells. Flow Cyt: HUVEC cells.
  • 常规说明

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • 纯度

    Protein A purified
  • 克隆

    单克隆
  • 克隆编号

    WM59
  • 同种型

    IgG1
  • 研究领域

    • Cardiovascular
    • Blood
    • Platelets
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Cell Adhesion Molecules
    • Endothelial
    • Cardiovascular
    • Angiogenesis
    • Angiogenic Factors
    • Stem Cells
    • Mesenchymal Stem Cells
    • Surface Molecules
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Cell adhesion
    • Other
    • Cancer
    • Tumor immunology
    • CD markers
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Leukocyte recruitment
    • Cell adhesion molecules
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • T Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Monocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Neutrophil Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Thrombocytic Lineage
    • Cardiovascular
    • Cardiovascular Markers
    • Cell Markers
    • Endothelial Cells
    • Cardiovascular
    • Angiogenesis
    • Endothelial Cell Markers

相关产品

  • Alternative Versions

    • Anti-CD31 antibody [WM59] - BSA and Azide free (ab252265)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
  • Related Products

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (ab150080)
    • Anti-beta Tubulin antibody [EPR16774] (ab179513)

应用

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab218 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

应用 Species
Flow Cyt
Human
ICC
Human
应用 Ab评论 说明
Flow Cyt
Use a concentration of 1.062 µg/ml.
ICC
Use a concentration of 10.62 µg/ml.

It is recommended to incubate cells with 0.1% Triton-X for 5 min to detect nuclear antigen. Use 0.3M glycine to quench autofluorescence caused by aldehydes.

Positive Control: HUVEC cells

说明
Flow Cyt
Use a concentration of 1.062 µg/ml.
ICC
Use a concentration of 10.62 µg/ml.

It is recommended to incubate cells with 0.1% Triton-X for 5 min to detect nuclear antigen. Use 0.3M glycine to quench autofluorescence caused by aldehydes.

Positive Control: HUVEC cells

  • 应用说明
    Is unsuitable for IHC-P.
  • 靶标

    • 功能

      Induces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC).
    • 组织特异性

      Expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Isoform Long predominates in all tissues examined. Isoform Delta12 is detected only in trachea. Isoform Delta14-15 is only detected in lung. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia (HEL) and U937 histiocytic lymphoma cell lines (at protein level).
    • 序列相似性

      Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
    • 结构域

      The Ig-like C2-type domains 2 and 3 contribute to formation of the complex with BDKRB2 and in regulation of its activity.
    • 翻译后修饰

      Phosphorylated on Ser and Tyr residues after cellular activation. In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation.
    • 细胞定位

      Membrane. Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells and Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells.
    • Target information above from: UniProt accession P16284 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 5175 Human
      • Omim: 173445 Human
      • SwissProt: P16284 Human
      • Unigene: 376675 Human
      • Unigene: 514412 Human
      • 别名

        • Adhesion molecule antibody
        • CD31 antibody
        • CD31 antigen antibody
        • CD31 EndoCAM antibody
        • EndoCAM antibody
        • FLJ34100 antibody
        • FLJ58394 antibody
        • GPIIA antibody
        • GPIIA' antibody
        • PECA1 antibody
        • PECA1_HUMAN antibody
        • Pecam 1 antibody
        • PECAM 1 CD31 EndoCAM antibody
        • PECAM antibody
        • PECAM-1 antibody
        • Pecam1 antibody
        • Platelet and endothelial cell adhesion molecule 1 antibody
        • Platelet endothelial cell adhesion molecule antibody
        • Platelet/endothelial cell adhesion molecule 1 antibody
        see all

      图片

      • Immunocytochemistry - Anti-CD31 antibody [WM59] (ab218)
        Immunocytochemistry - Anti-CD31 antibody [WM59] (ab218)

        Immunocytochemistry analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HUVEC cells labelling CD31 with ab218 at 1/100 dilution (10.62µg/ml), followed by ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 (2µg/ml) dilution (Green). Confocal image showing cytoplasmic and membranous staining in HUVEC cell line. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/500 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at a 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).

        Negative control cell: HEK-293 (PMID: 27097314).

        Negative control 1: ab218 at a 1/100 dilution (10.62µg/ml) followed by ab150080 at a 1/500 dilution (4µg/ml).

        Negative control 2: ab179513 at a 1/500 dilution (4µg/ml) followed by ab150113 at a 1/1000 dilution (4µg/ml).

         

      • Flow Cytometry - Anti-CD31 antibody [WM59] (ab218)
        Flow Cytometry - Anti-CD31 antibody [WM59] (ab218)

        Flow cytometric analysis of HEK 293 (human embryonic kidney epithelial cell) (Left panel) / HUVEC (human umbilical vein endothelial cell) (Right panel) cells labelling CD31 with ab218 at 1/1000 dilution (1.062µg/ml) (Red) compared with a mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution was used as the secondary antibody.

        Negative control: HEK 293 (PMID: 27097314).

        Gated on viable cells.

      • Anti-CD31 antibody [WM59] (ab218)
        Anti-CD31 antibody [WM59] (ab218)

      实验方案

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      数据表及文件

      • SDS download

      • Datasheet download

        Download

      文献 (6)

      发表研究结果有使用 ab218?请让我们知道,以便我们可以引用本数据表中的参考文章。

      ab218 被引用在 6 文献中.

      • Lundqvist A  et al. The Arachidonate 15-Lipoxygenase Enzyme Product 15-HETE Is Present in Heart Tissue from Patients with Ischemic Heart Disease and Enhances Clot Formation. PLoS One 11:e0161629 (2016). PubMed: 27552229
      • Bushway ME  et al. Morphological and phenotypic analyses of the human placenta using whole mount immunofluorescence. Biol Reprod 90:110 (2014). PubMed: 24648400
      • Wicki A  et al. Targeting tumor-associated endothelial cells: anti-VEGFR2 immunoliposomes mediate tumor vessel disruption and inhibit tumor growth. Clin Cancer Res 18:454-64 (2012). PubMed: 22065082
      • Oeztuerk-Winder F  et al. Regulation of human lung alveolar multipotent cells by a novel p38a MAPK/miR-17-92 axis. EMBO J 31:3431-41 (2012). PubMed: 22828869
      • Nagano M  et al. Identification of functional endothelial progenitor cells suitable for the treatment of ischemic tissue using human umbilical cord blood. Blood 110:151-60 (2007). PubMed: 17379743
      • MacFadyen JR  et al. Endosialin (TEM1, CD248) is a marker of stromal fibroblasts and is not selectively expressed on tumour endothelium. FEBS Lett 579:2569-75 (2005). PubMed: 15862292

      客户评价及客户问答

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      1-3 of 3 Abreviews or Q&A

      Immunocytochemistry/ Immunofluorescence abreview for Anti-CD31 antibody [WM59]

      Average
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (HT1080 cells)
      Permeabilization
      Yes - 0.1% Triton X-100
      Specification
      HT1080 cells
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      提交于 Jan 26 2010

      Question

      I'm trying to find an antibody to CD31 that will recognize RAT epithelial cells. I was wondering if your CD31 clone WM59 antibody has been tested in IHC-P applications (it's not listed under the applications and I'd like to know if this means it just hasn't been tested or if it has been tested and found not to work). Thank you.

      Read More

      Abcam community

      Verified customer

      Asked on Feb 18 2004

      Answer

      Thank you for your enquiry. To our knowledge, this antibody has yet to be tested in this application (IHC-P). All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, you may wish to participate in our AbTrial program for untested species and applications. For more information see www.abcam.com/abtrial

      Read More

      Abcam Scientific Support

      回复于 Feb 18 2004

      Question

      Does this antibody cross react with pig tissue? Can I use this antibody for immunohistochemistry ?

      Read More

      Abcam community

      Verified customer

      Asked on Mar 14 2001

      Answer

      Sorry, cross-reactivity of this antibody with other species is not yet known and it has not been tested in immunohistochemistry.

      Read More

      Abcam Scientific Support

      回复于 Mar 14 2001

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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