Application
IHC - Wholemount
Sample
Mouse Tissue (ear skin)
Specification
ear skin
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.1% BSA
Dilution
1/300
Additional data
Additional Notes
4% PFA fixation.
No antigen retrieval. 0.2% Triton X-100 in PB for permeabilization.
Abcam response
Thank you for your valuable feedback. According to the user and the result image, this antibody (ab7388) gave non-specific staining to the hairs rather than the blood vessels. We have not tested ab7388 in IHC-wholemount previously. It is possible that this antibody may not be suitable for use in this application. We welcome other Abreviews to give further supporting data on this.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Dec 28 2020