Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD31 antibody

Good
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: sodium citrate pH 8.5 80 C
Permeabilization
Yes - 0.3% triton X-100
Specification
Kidney
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
10%NBF

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/50

Secondary antibody

Name
Non-Abcam antibody was used: Vectastain Biotinylated Universal Antibody
Host species: Horse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Additional data

Additional Notes
Also tested on vertebrae sections, poor staining on bone compared to kidney, may need to use for immunofluoresence instead.

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提交于 May 02 2019

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