Western blot abreview for Anti-CD31 antibody

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (human vascular endothelial cells)
Loading amount
25 µg
Specification
human vascular endothelial cells
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Other product details

Dilution
1/500
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: 5% Marvel TBS-T

Secondary antibody

Name
Non-Abcam antibody was used: anti-rabbit HRP
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Detection

Detection method
ECL+
Exposure
12 minute(s) and 0 second(s)
Bands
Specific: 90, 110 kDa

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提交于 Mar 04 2010

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