概述

  • 产品名称
  • 描述
    兔多克隆抗体to CD31
  • 宿主
    Rabbit
  • 特异性
    Customer feedback indicates that there might be some batch-to-batch variation with respect to mouse cross-reactivity. We recommend ab124432 as an alternative antibody for mouse samples. Please contact Abcam if you have any queries.
  • 经测试应用
    适用于: IHC-Fr, IHC-P, ICC/IF, IHC-FoFr, WBmore details
  • 种属反应性
    与反应: Mouse, Human, Pig
  • 免疫原

    Synthetic peptide within Mouse CD31 aa 700 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: Q08481

  • 阳性对照
    • IHC-P: Mouse skin, xenograft tissue; Human tonsil tissue. WB: Jurkat whole cell lysate (ab7899), Human vascular endothelial cell lysate. IHC-Fr: Human fetal heart tissue.
  • 常规说明

      

应用

Our Abpromise guarantee covers the use of ab28364 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr 1/50.
IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/20. PubMed: 17316380
IHC-FoFr Use at an assay dependent concentration.
WB 1/500.

靶标

  • 功能
    Induces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC).
  • 组织特异性
    Expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Isoform Long predominates in all tissues examined. Isoform Delta12 is detected only in trachea. Isoform Delta14-15 is only detected in lung. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia (HEL) and U937 histiocytic lymphoma cell lines (at protein level).
  • 序列相似性
    Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
  • 结构域
    The Ig-like C2-type domains 2 and 3 contribute to formation of the complex with BDKRB2 and in regulation of its activity.
  • 翻译后修饰
    Phosphorylated on Ser and Tyr residues after cellular activation. In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation.
  • 细胞定位
    Membrane. Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells and Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Adhesion molecule antibody
    • CD31 antibody
    • CD31 antigen antibody
    • CD31 EndoCAM antibody
    • EndoCAM antibody
    • FLJ34100 antibody
    • FLJ58394 antibody
    • GPIIA antibody
    • GPIIA' antibody
    • PECA1 antibody
    • PECA1_HUMAN antibody
    • Pecam 1 antibody
    • PECAM 1 CD31 EndoCAM antibody
    • PECAM antibody
    • PECAM-1 antibody
    • Pecam1 antibody
    • Platelet and endothelial cell adhesion molecule 1 antibody
    • Platelet endothelial cell adhesion molecule antibody
    • Platelet/endothelial cell adhesion molecule 1 antibody
    see all

图片

  • ab28364 at 1/50 dillution staining CD31 in human tonsil tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin embedded tissue sections).

  • ab28364 at a 1/300 dilution, staining human CD31 in fetal heart tissue by Immunohistochemistry, frozen tissue.

    See Abreview

  • Anti-CD31 antibody (ab28364) at 1/50 dilution staining mouse tissue sections by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). Following heat mediated antigen retrieval the tissue was formaldehyde fixed and blocked with serum. The tissue was incubated with the primary antibody for 20 minutes. A biotinylated goat anti-rabbit antibody was used as the secondary. The endothelium of vessels of any tissues are positive.

    See Abreview

  • Anti-CD31 antibody (ab28364) at 1/500 dilution + Human vascular endothelial cells at 25 µg

    Secondary
    anti-rabbit HRP at 1/5000 dilution

    See Abreview

  • Representative images of cross sections with CD31 staining in red, nucleus in blue. White dotted lines indicate the graft wall. Scale bar = 100 μm.

    Explanted grafts from C57/BL6 mice were fixed overnight in paraformaldehyde (2%) at room temperature. Samples were dehydrated through an ethanol gradient and embedded in paraffin and sectioned at 5 μm transversely from proximal anastomosis to distal anastomosis. For immunohistochemistry staining, sections were deparaffinised and stained with antibody against CD31 (Abcam, ab28364, 1:200) for endothelial cells.

     

  • ab28364 staining CD31 in mouse xenograft tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde, cut into 20 micron slices and blocked with 1% BSA for 60 minutes at 21°C. Antigen retrieval was by heat mediation in a Tris/EDTA pH9 buffer. The sample was incubated with primary antibody (20 ug/mL) at 4°C for 16 hours. A HRP-conjugated goat anti-rabbit polyclonal (2 ug/mL) was used as the secondary antibody.

    See Abreview

  • GFP positive cells (green) were colocalized with CD31 (red). Nuclear staining with DAPI is blue.

    For immunofluorescence staining, the skin samples were fixed in 4% paraformaldehyde and embedded in paraffin. Skin sections were stained with an anti-GFP antibody. The skin sections were also treated with primary Abs against CD31 (Abcam, ab28364, diluted 1:300). Secondary antibodies conjugated to rhodamine-isothiocyanate were used for fluorescence detection on a confocal laser scanning fluorescence microscope (Zeiss, German).

    Opn-/-; OPN (Osteopontin) knock-out mice.

文献

This product has been referenced in:
  • Xu L  et al. ATM deficiency promotes progression of CRPC by enhancing Warburg effect. Endocr Relat Cancer 26:59-71 (2019). Read more (PubMed: 30400006) »
  • Gallina D & Lincoln J Dynamic Expression Profiles of Sox9 in Embryonic, Post Natal, and Adult Heart Valve Cell Populations. Anat Rec (Hoboken) 302:108-116 (2019). Read more (PubMed: 30412364) »
See all 948 Publications for this product

客户评价及客户问答

1-10 of 31 Q&A

Answer

We have heard positive feedback from a researcher who used ab28364 in sheep lung tissue in IHC. You can read the Abreview here: https://www.abcam.com/cd31-antibody-ab28364.html?productWallTab=Abreviews#cd31-antibody-ab28364.html?productWallTab=Abreviews&species=4&_suid=141660436745707317365168763169


That's the only usage of this antibody in sheep that we know of thus far. SwissProt has 2 putative sequences for this protein in sheep, and I BLASTed the immunogen region (from mouse) and it shows an 80 - 86% homology with sheep. Generally we say anything with greater than 85% sequence homology would be expected to cross-react, so you have a shot here!

Read More

Question
Answer

I performed a blast on the bovine and mouse sequence for PECAM1 and it showed a homology of less that 70%, so its unlikely that this antibody will work in bovine samples.

Read More

Question
Answer

The isotype controls are as follows:
ab8158 -- ab18450 rat IgG2a
ab51067 -- ab125938 rabbit monoclonal
ab28364 -- ab27472 rabbit polyclonal

Read More

Question
Answer

Yes, ab28365 is the diluted version of ab28364.

Read More

Answer

Because we carry over 90,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.

We like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

To find out more about our Abreview system, please see the following link:

https://www.abcam.com/abreviews

Having looked over these Abreviews for ab28364, many customers have had great success of using IHC-P in mice. Although none have tested the antibody on mouse placenta directly, we have several reports of it giving good results when using mouse liver, spleen, skin, brain, embryo, lung, heart and kidney. Therefore, there should be no reason why ab28364 shouldn't work on mouse placenta. However, as already mentioned if you receive a batch which doesn't work according to our datasheet, we would be happy to replace or refund the antibody.

Read More

Answer

We have removed rat from the predicted species, because we received several complaints. We continue monitoring to find out if the complaint rate is reduced in time.
I would encourage your customer to submit an Abreview to share the results with the research community.

Read More

Answer

We do have two monoclonal antibodies to CD31 that are reactive with rat and are tested for Immunohistochemistry, ab24590 and ab64543.

Read More

Question
Answer

No, ab28364 is not on this list of species reactivity, therefore we would not recommend it to work in rat. However, we wold recommend ab24590 or ab64543 for a anti-CD31 antibody which reacts with rat.

Read More

Answer

We don't have an in-house protocol for testing this antibody in IHC-Fr, as this application was added to the datasheet based on feedback from users. I've looked through our Abreviews (linked below) to find out how other users have successfully used the antibody and I've compiled the information below-

https://www.abcam.com/CD31-antibody-ab28364-reviews.html#IHC-Fr

1) Fixation: Acetone
2) Incubation buffer: TBST with 2% BSA and 0.2% azide; PBS with 10% goat serum and 0.01% Triton and 0.1% saponin; PBS with 5% goat serum and 1% BSA
3) Blocking buffer: 10-20% serum (unknown source); 1% BSA
4) Secondary antibody and concentration and substrate recommendations: Anti-rabbit HRP staining kit (others used fluorescent secondaries)

Regarding the blocking serum, I suggest using serum from the host of your secondary antibody, i.e. if you use a goat anti-rabbit secondary then use goat serum for blocking. This primary antibody should work with any anti-rabbit secondary antibody and I'd suggest using DAB detection. We have several HRP conjugated secondaries that will work, such as ab6802-

https://www.abcam.com/Donkey-polyclonal-Secondary-Antibody-to-Rabbit-IgG-HL-HRP-ab6802.html

In this case you could use donkey serum for blocking and DAB substrate for detection-

https://www.abcam.com/Donkey-Serum-ab7475.html

https://www.abcam.com/DAB-Substrate-Kit-ab64238.html

Read More

Answer

While we do not recommend freezing this product, aliquoting any antibody is a good idea as this can prevent issues such as contamination from arising.

Read More

1-10 of 31 Q&A

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

注册